Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae

Esraa S. Ethafa; Alaa J. A.  Al-Manhel

doi:10.37077/25200860.2022.35.2.03

Article Details

Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae

Authors:

Esraa S. Ethafa | Alaa J. A. Al-Manhel

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

Screening

Journal:

Basrah Journal of Agricultural Sciences Vol.35 Issue 2, pp.34-48

Document Type:

Article

Publication Date:

8/2022

Document Language:

English

Document ID (Org No.):

2001363621

pISSN:

2520-0860

DOI:

10.37077/25200860.2022.35.2.03

Keywords:

Abstract:

 Ergosterol is an important provitamin in the present-day of industrial biotechnology. Seven yeast strains were obtained from the market of Basrah city and subjected to screening for their ergosterol production ability using liquid-state fermentation, the cultural conditions, and nutritional requirements for optimal production of ergosterol by Saccharomyces cerevisiae under laboratory conditions were determined. Y.6 is the best isolate of the yeast that produced ergosterol. It was identified as S. cerevisiae, with a similarity rate of 97% by using the Vitek2 device, this S. cerevisiae (Y.6) was further subjected to optimization conditions. The results showed that the best medium for production was yeast extract peptone dextrose broth. The effect of two cheaper carbon sources, molasses and date juice were investigated. Maximum ergosterol (0.55% ) was produced using a medium containing date juice, with a replacement ratio of 75%, an incubation time of 72 hours, pH 5, at a temperature of 30 °C, and an inoculation volume of 4 ml. The percentage of ergosterol was (0.47, 0.55, 0.74, 0.66, 0.68 and 0.78 %), respectively.

Article Details

Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae

Authors:

Esraa S. Ethafa | Alaa J. A. Al-Manhel

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

Screening

Journal:

Basrah Journal of Agricultural Sciences Vol.35 Issue 2, pp.34-48

Document Type:

Article

Publication Date:

8/2022

Document Language:

English

Document ID (Org No.):

2001363621

pISSN:

2520-0860

DOI:

10.37077/25200860.2022.35.2.03

Ergosterol production

Optimum conditions

Saccharomyces cerevisiae

Screening

Issues

Article Details

Determination of Optimum Conditions for Ergosterol Production by Saccharomyces cerevisiae

Authors: Esraa S. Ethafa | Alaa J. A. Al-Manhel

Publisher: College of Agriculture, University of Basrah, Iraq

Main Topics: Screening

Journal: Basrah Journal of Agricultural Sciences Vol.35 Issue 2, pp.34-48

Document Type: Article

Publication Date: 8/2022

Document Language: English

Document ID (Org No.): 2001363621

pISSN: 2520-0860

DOI: 10.37077/25200860.2022.35.2.03

Ergosterol production

Optimum conditions

Saccharomyces cerevisiae

Screening

Issues

Authors:

Esraa S. Ethafa | Alaa J. A. Al-Manhel

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

Screening

Journal:

Basrah Journal of Agricultural Sciences Vol.35 Issue 2, pp.34-48

Document Type:

Article

Publication Date:

8/2022

Document Language:

English

Document ID (Org No.):

2001363621

pISSN:

2520-0860

DOI:

10.37077/25200860.2022.35.2.03