Evaluation of the Effects of Imazalil on Genotoxicity and Behavioral Toxicity in Drosophila melanogaster

Sezin Özdemir; Mehmet Furkan Çilek; Selcan Girgin; Özkan Şeşen; Dilek Akyıl; Arzu Özkara; İbrahim Hakkı Ciğerci

Article Details

Evaluation of the Effects of Imazalil on Genotoxicity and Behavioral Toxicity in Drosophila melanogaster

Authors:

Sezin Özdemir | Mehmet Furkan Çilek | Selcan Girgin | Özkan Şeşen | Dilek Akyıl | Arzu Özkara | İbrahim Hakkı Ciğerci

Publisher:

Afyon Kocatepe University

Main Topics:

Yapısal Biyoloji

Journal:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.476-484

Document Type:

Article

Publication Date:

6/2024

Document Language:

English

Document ID (Org No.):

2001317576

pISSN:

2149-3367

Keywords:

Abstract:

 Imazalil IMZ is an imidazole and triazole derivative fungicide that is widely used to prevent many diseases in vegetable and fruit fields and to prevent post-harvest spoilage. In this study, the genotoxic potential of IMZ at different concentrations 0.25, 1, 4.5 mM on Drosophila melanogaster was investigated using Somatic Mutation and Recombination SMART and Single Cell Gel Electrophoresis Comet Assays. The effect of the same IMZ concentrations on behavioral toxicity in D. melanogaster was investigated. Larval weight, crawling, and pupa formation success were performed to determine behavioral toxicity. As a result of the study, it was determined that IMZ generally caused a negative effect on D. melanogaster. In the SMART test, it was found that the differences between the wing preparations of the individuals obtained as a result of all IMZ concentration applications were not statistically significant compared to the negative control. The damage caused to DNA by IMZ was determined by the Comet test, and a statistically significant increase in DNA damage scores was observed at doses of 1 and 4.5 mM. In the crawling experiment of IMZ on D. melanogaster, a decrease in locomotion occurred due to the increase in dose compared to the control group, and these changes were found to be statistically significant at all application doses. Changes in larval weight were not found to be statistically significant. In the pupa formation success experiment, the decrease at 1 and 4.5 mM doses was found to be statistically significant.

 Imazalil IMZ is an imidazole and triazole derivative fungicide that is widely used to prevent many diseases in vegetable and fruit fields and to prevent post-harvest spoilage. In this study, the genotoxic potential of IMZ at different concentrations 0.25, 1, 4.5 mM on Drosophila melanogaster was investigated using Somatic Mutation and Recombination SMART and Single Cell Gel Electrophoresis Comet Assays. The effect of the same IMZ concentrations on behavioral toxicity in D. melanogaster was investigated. Larval weight, crawling, and pupa formation success were performed to determine behavioral toxicity. As a result of the study, it was determined that IMZ generally caused a negative effect on D. melanogaster. In the SMART test, it was found that the differences between the wing preparations of the individuals obtained as a result of all IMZ concentration applications were not statistically significant compared to the negative control. The damage caused to DNA by IMZ was determined by the Comet test, and a statistically significant increase in DNA damage scores was observed at doses of 1 and 4.5 mM. In the crawling experiment of IMZ on D. melanogaster, a decrease in locomotion occurred due to the increase in dose compared to the control group, and these changes were found to be statistically significant at all application doses. Changes in larval weight were not found to be statistically significant. In the pupa formation success experiment, the decrease at 1 and 4.5 mM doses was found to be statistically significant.

 Imazalil IMZ is an imidazole and triazole derivative fungicide that is widely used to prevent many diseases in vegetable and fruit fields and to prevent post-harvest spoilage. In this study, the genotoxic potential of IMZ at different concentrations 0.25, 1, 4.5 mM on Drosophila melanogaster was investigated using Somatic Mutation and Recombination SMART and Single Cell Gel Electrophoresis Comet Assays. The effect of the same IMZ concentrations on behavioral toxicity in D. melanogaster was investigated. Larval weight, crawling, and pupa formation success were performed to determine behavioral toxicity. As a result of the study, it was determined that IMZ generally caused a negative effect on D. melanogaster. In the SMART test, it was found that the differences between the wing preparations of the individuals obtained as a result of all IMZ concentration applications were not statistically significant compared to the negative control. The damage caused to DNA by IMZ was determined by the Comet test, and a statistically significant increase in DNA damage scores was observed at doses of 1 and 4.5 mM. In the crawling experiment of IMZ on D. melanogaster, a decrease in locomotion occurred due to the increase in dose compared to the control group, and these changes were found to be statistically significant at all application doses. Changes in larval weight were not found to be statistically significant. In the pupa formation success experiment, the decrease at 1 and 4.5 mM doses was found to be statistically significant.

 İmazalil IMZ, sebze ve meyve tarlalarında birçok hastalığın önlenmesi ve hasat sonrası bozulmanın engellenmesi amacıyla yaygın olarak kullanılan bir imidazol ve triazol türevi fungisittir. Bu çalışmada, farklı konsantrasyonlardaki 0.25, 1, 4.5 mM IMZnin Drosophila melanogaster üzerindeki genotoksik potansiyeli Somatik Mutasyon ve Rekombinasyon SMART ve Tek Hücreli Jel Elektroforez Comet Testleri kullanılarak incelenmiştir. Aynı IMZ konsantrasyonlarının D. melanogasterde davranışsal toksisiteye etkisi de araştırılmıştır. Larva ağırlığı, sürünme ve pupa oluşum başarısı davranışsal toksisiteyi belirlemek için gerçekleştirilmiştir. Çalışma sonucunda, IMZnin genel olarak D. melanogaster üzerinde olumsuz etkilere neden olduğu belirlenmiştir. SMART testinde, tüm IMZ konsantrasyonu uygulamalarının elde edilen bireylerin kanat preparasyonları arasındaki farkların negatif kontrolle istatistiksel olarak anlamlı olmadığı bulunmuştur. IMZ tarafından DNAya verilen hasar, Comet testi ile belirlenmiş, 1 ve 4.5 mMlık dozlarda istatistiksel olarak DNA hasar skorlarında anlamlı bir artış gözlenmiştir. IMZnin D. melanogaster üzerinde yapılan sürünme deneyinde kontrol grubuna kıyasla doz artışına bağlı olarak hareket kabiliyetinde azalma meydana gelmiş ve bu değişiklikler tüm uygulama dozlarında istatistiksel olarak anlamlı bulunmuştur. Larva ağırlığındaki değişiklikler ise istatistiksel olarak anlamlı bulunmamıştır. Pupa oluşum başarısı deneyinde ise 1 ve 4.5 mMlık dozlardaki azalış istatistiksel olarak anlamlı bulunmuştur.

Article Details

Evaluation of the Effects of Imazalil on Genotoxicity and Behavioral Toxicity in Drosophila melanogaster

Authors:

Sezin Özdemir | Mehmet Furkan Çilek | Selcan Girgin | Özkan Şeşen | Dilek Akyıl | Arzu Özkara | İbrahim Hakkı Ciğerci

Publisher:

Afyon Kocatepe University

Main Topics:

Yapısal Biyoloji

Journal:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.476-484

Document Type:

Article

Publication Date:

6/2024

Document Language:

English

Document ID (Org No.):

2001317576

pISSN:

2149-3367

Behavioral cytotoxicity

COMET test

Drosophila melanogaster

Imazalil

SMART testCOMET testi

Davranışsal sitotoksisite

Drosophila melanogaster

İmazalil

SMART testi

Issues

Article Details

Authors: Sezin Özdemir | Mehmet Furkan Çilek | Selcan Girgin | Özkan Şeşen | Dilek Akyıl | Arzu Özkara | İbrahim Hakkı Ciğerci

Publisher: Afyon Kocatepe University

Main Topics: Yapısal Biyoloji

Journal: Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.476-484

Document Type: Article

Publication Date: 6/2024

Document Language: English

Document ID (Org No.): 2001317576

pISSN: 2149-3367

Issues

Authors:

Sezin Özdemir | Mehmet Furkan Çilek | Selcan Girgin | Özkan Şeşen | Dilek Akyıl | Arzu Özkara | İbrahim Hakkı Ciğerci

Publisher:

Afyon Kocatepe University

Main Topics:

Yapısal Biyoloji

Journal:

Afyon Kocatepe Üniversitesi Fen Ve Mühendislik Bilimleri Dergisi Vol.24 Issue 3, pp.476-484

Document Type:

Article

Publication Date:

6/2024

Document Language:

English

Document ID (Org No.):

2001317576

pISSN:

2149-3367