Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method

Ferda Dondurmacıoğlu

Article Details

Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method

Authors:

Ferda Dondurmacıoğlu

Publisher:

Çanakkale Onsekiz Mart University

Main Topics:

Food Engineering

Journal:

Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.506-519

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001328938

pISSN:

2757-5195

Keywords:

Abstract:

 Among the reactive oxygen species, Superoxide radicals can produce dangerous species that cause lipid peroxidation. Therefore, the determination and scavenging of superoxide radicals is critical. Our study is based on the interaction of the superoxide radical produced from the β-Nicotinamide adenine dinucleotide reduced disodium salt hydrate and phenazine methosulfate NADH-PMS system with N, N-dimethyl-p-phenylenediamine dihydrochloride DMPD to form the pink colored DMPD-quinone DMPDQ radical. In the presence of scavengers with superoxide radical scavenging activity antioxidants, herbal teas the color intensity decreases due to reduced DMPDQ radical production. The absorbance of the colored reference solution and the sample solution containing the radical scavenger was measured at 552 nm. The difference in absorbance ΔA between the reference solution and the sample solution was found. ΔA is proportional to the scavenger concentration. In the study, the superoxide radical scavenging effect of trolox TR and different AOXs was investigated. The superoxide radical scavenging effect of three different herbal tea infusion solutions was measured with this method. From the graph drawn between herbal tea concentrations and percentage inhibition values, 50 inhibition EC50 values of herbal teas were found. EC50 method values were compared with the EC50 values of the nitroblue tetrazolium NBT and the 2,2-azino-bis3-etilbenzotiyazolin-6-sülfonik asit ABTS method. In addition, ABTS, cupric reducing antioxidant capacity CUPRAC, and this study total antioxidant capacity TAC values of herbal tea infusions were calculated and compared.

 Among the reactive oxygen species, Superoxide radicals can produce dangerous species that cause lipid peroxidation. Therefore, the determination and scavenging of superoxide radicals is critical. Our study is based on the interaction of the superoxide radical produced from the β-Nicotinamide adenine dinucleotide reduced disodium salt hydrate and phenazine methosulfate NADH-PMS system with N, N-dimethyl-p-phenylenediamine dihydrochloride DMPD to form the pink colored DMPD-quinone DMPDQ radical. In the presence of scavengers with superoxide radical scavenging activity antioxidants, herbal teas the color intensity decreases due to reduced DMPDQ radical production. The absorbance of the colored reference solution and the sample solution containing the radical scavenger was measured at 552 nm. The difference in absorbance ΔA between the reference solution and the sample solution was found. ΔA is proportional to the scavenger concentration. In the study, the superoxide radical scavenging effect of trolox TR and different AOXs was investigated. The superoxide radical scavenging effect of three different herbal tea infusion solutions was measured with this method. From the graph drawn between herbal tea concentrations and percentage inhibition values, 50 inhibition EC50 values of herbal teas were found. EC50 method values were compared with the EC50 values of the nitroblue tetrazolium NBT and the 2,2-azino-bis3-etilbenzotiyazolin-6-sülfonik asit ABTS method. In addition, ABTS, cupric reducing antioxidant capacity CUPRAC, and this study total antioxidant capacity TAC values of herbal tea infusions were calculated and compared.

 Among the reactive oxygen species, Superoxide radicals can produce dangerous species that cause lipid peroxidation. Therefore, the determination and scavenging of superoxide radicals is critical. Our study is based on the interaction of the superoxide radical produced from the β-Nicotinamide adenine dinucleotide reduced disodium salt hydrate and phenazine methosulfate NADH-PMS system with N, N-dimethyl-p-phenylenediamine dihydrochloride DMPD to form the pink colored DMPD-quinone DMPDQ radical. In the presence of scavengers with superoxide radical scavenging activity antioxidants, herbal teas the color intensity decreases due to reduced DMPDQ radical production. The absorbance of the colored reference solution and the sample solution containing the radical scavenger was measured at 552 nm. The difference in absorbance ΔA between the reference solution and the sample solution was found. ΔA is proportional to the scavenger concentration. In the study, the superoxide radical scavenging effect of trolox TR and different AOXs was investigated. The superoxide radical scavenging effect of three different herbal tea infusion solutions was measured with this method. From the graph drawn between herbal tea concentrations and percentage inhibition values, 50 inhibition EC50 values of herbal teas were found. EC50 method values were compared with the EC50 values of the nitroblue tetrazolium NBT and the 2,2-azino-bis3-etilbenzotiyazolin-6-sülfonik asit ABTS method. In addition, ABTS, cupric reducing antioxidant capacity CUPRAC, and this study total antioxidant capacity TAC values of herbal tea infusions were calculated and compared.

 Reaktif oksijen türleri içinde yer alan süperoksit radikalleri lipid peroksidasyonuna neden olan tehlikeli türler üretebilir. Bu nedenle süperoksit radikali tayini, süpürülmesi çok önemlidir. Çalışmamız β-Nicotinamid adenin dinükleotid indirgenmiş disodyum tuz hidratı ve fenazin metosulfat NADH-PMS sisteminden üretilen süperoksit radikalinin N, N-dimetil-p-fenilendiamin dihidroklorür DMPD ile etkileşimi sonucu pembe renkli DMPD-yarıkinon DMPDQ radikali oluşturmasına dayanmaktadır. Süperoksit radikali giderme aktivitesine sahip süpürücüler antioksidan, bitki çayları varlığında daha az DMPDQ radikali üretimi nedeniyle renk yoğunluğu azalır. Renkli referans çözeltisinin ve radikal süpürücü içeren örnek çözeltisinin absorbansı 552 nmde ölçülmüştür. Referans çözelti ile örnek çözeltisinin absorbans farkı ΔA bulunmuştur. ΔA ile süpürücü derişimi orantılıdır. Çalışmada, trolox TR ve farklı antioksidanların AOX süperoksit radikal süpürücü etkisi araştırılmıştır. Yöntemle üç farklı bitki çayı infüzyon çözeltisinin süperoksit radikal süpürücü etkisi ölçülmüştür. Bitki çayı derişimleri ile yüzde inhibisyon değerleri arasında çizilen grafikten bitki çaylarının 50 inhibisyon EC50 değerleri bulunmuştur. Bulunan EC50 değerleri süperoksit radikal tayininde yaygın olarak kullanılan nitroblue tetrazolium NBT ve 2,2-azino-bis3-etilbenzotiyazolin-6-sülfonik asit ABTS yöntemi EC50 değerleriyle karşılaştırılmıştır. Ayrıca bitki çayı infüzyonlarının ABTS, bakırII iyonu indirgeme antioksidan kapasite CUPRAC yöntemi ve çalışılan yöntemde toplam antioksidan kapasite değerleri TAC hesaplanıp, karşılaştırılmıştır.

Article Details

Determination of Superoxide Radical Scavenging Activity and Total Antioxidant Capacity by Modified DMPD Method

Authors:

Ferda Dondurmacıoğlu

Publisher:

Çanakkale Onsekiz Mart University

Main Topics:

Food Engineering

Journal:

Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.506-519

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001328938

pISSN:

2757-5195

Antioxidant capacity

NADH-PMS

N N-dimethyl-p-phenylenediamine dihydrochloride

superoxide radical

percentage inhibitionAntioksidan kapasite

NADH-PMS

N N-dimetil-p-fenilendiamin dihidroklorür

süperoksit radikali

yüzde inhibisyon

Issues

Article Details

Authors: Ferda Dondurmacıoğlu

Publisher: Çanakkale Onsekiz Mart University

Main Topics: Food Engineering

Journal: Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.506-519

Document Type: Article

Publication Date: 9/2024

Document Language: English

Document ID (Org No.): 2001328938

pISSN: 2757-5195

Issues

Authors:

Ferda Dondurmacıoğlu

Publisher:

Çanakkale Onsekiz Mart University

Main Topics:

Food Engineering

Journal:

Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.506-519

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001328938

pISSN:

2757-5195