Preventive Effect of Edaravone Against Ischemia-Reperfusion Injury in Rat Epigastric Island Flaps: An Experimental Study

Ovunc Akdemir; Burak Ergün Tatar; Yiğit Uyanıkgil; Oytun Erbaş; Feng Zhang; William C. Lineaweaver

Article Details

Preventive Effect of Edaravone Against Ischemia-Reperfusion Injury in Rat Epigastric Island Flaps: An Experimental Study

Authors:

Ovunc Akdemir | Burak Ergün Tatar | Yiğit Uyanıkgil | Oytun Erbaş | Feng Zhang | William C. Lineaweaver

Publisher:

Selcuk University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Genel Tıp Dergisi Vol.33 Issue 2, pp.117-122

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001288864

pISSN:

2602-3741

Keywords:

Abstract:

 Objective Ischemia-reperfusion IR injury is tissue damage occurring when blood returns to a tissue after an ischemic phase. Reperfusion initiates a cascade of acute inflammatory processes that end in cell death, tissue malfunction, and necrosis. Edaravone 3-methy-1-pheny1-2-pyrazolin-5-one is a powerful and unique synthetic radical scavenger. In this research, the effects of edaravone on IR damage were investigated. Material and Methods: 16 adult male SpragueDawley rats were utilized. Eight rats were allocated at random into two groups. Group 1 the control group experienced ischemia and reperfusion of an abdominal skin flap for 10 hours without therapy. Group 2 treatment group received an intraperitoneal injection of 3 mgkg edaravone 10 hours prior to ischemia and reperfusion in an abdominal skin flap. Using planimetry, flaps were examined at intervals of 1, 3, 7, and 10 days. Then, flaps were removed for biochemical measurement of tissue glutathione GSH, tissue protein, lipid peroxidation MDA, and nitric oxide NO levels and histopathological measurement of tissue glutathione GSH, tissue protein, lipid peroxidation MDA, and nitric oxide NO levels Results: We found no significant changes p  0.05 between groups 1 and 2 in terms of NO, MDA, GSH, or planimetric analyses. Group 2 had a lower neutrophil count than group 1, however the difference was not statistically significant p  0.05. Conclusion Edaravone is a very effective antioxidant. Nevertheless, our research indicates that it may not influence IR damage in a skin flap model.

 Objective Ischemia-reperfusion IR injury is tissue damage occurring when blood returns to a tissue after an ischemic phase. Reperfusion initiates a cascade of acute inflammatory processes that end in cell death, tissue malfunction, and necrosis. Edaravone 3-methy-1-pheny1-2-pyrazolin-5-one is a powerful and unique synthetic radical scavenger. In this research, the effects of edaravone on IR damage were investigated. Material and Methods: 16 adult male SpragueDawley rats were utilized. Eight rats were allocated at random into two groups. Group 1 the control group experienced ischemia and reperfusion of an abdominal skin flap for 10 hours without therapy. Group 2 treatment group received an intraperitoneal injection of 3 mgkg edaravone 10 hours prior to ischemia and reperfusion in an abdominal skin flap. Using planimetry, flaps were examined at intervals of 1, 3, 7, and 10 days. Then, flaps were removed for biochemical measurement of tissue glutathione GSH, tissue protein, lipid peroxidation MDA, and nitric oxide NO levels and histopathological measurement of tissue glutathione GSH, tissue protein, lipid peroxidation MDA, and nitric oxide NO levels Results: We found no significant changes p  0.05 between groups 1 and 2 in terms of NO, MDA, GSH, or planimetric analyses. Group 2 had a lower neutrophil count than group 1, however the difference was not statistically significant p  0.05. Conclusion Edaravone is a very effective antioxidant. Nevertheless, our research indicates that it may not influence IR damage in a skin flap model.

 Objective Ischemia-reperfusion IR injury is tissue damage occurring when blood returns to a tissue after an ischemic phase. Reperfusion initiates a cascade of acute inflammatory processes that end in cell death, tissue malfunction, and necrosis. Edaravone 3-methy-1-pheny1-2-pyrazolin-5-one is a powerful and unique synthetic radical scavenger. In this research, the effects of edaravone on IR damage were investigated. Material and Methods: 16 adult male SpragueDawley rats were utilized. Eight rats were allocated at random into two groups. Group 1 the control group experienced ischemia and reperfusion of an abdominal skin flap for 10 hours without therapy. Group 2 treatment group received an intraperitoneal injection of 3 mgkg edaravone 10 hours prior to ischemia and reperfusion in an abdominal skin flap. Using planimetry, flaps were examined at intervals of 1, 3, 7, and 10 days. Then, flaps were removed for biochemical measurement of tissue glutathione GSH, tissue protein, lipid peroxidation MDA, and nitric oxide NO levels and histopathological measurement of tissue glutathione GSH, tissue protein, lipid peroxidation MDA, and nitric oxide NO levels Results: We found no significant changes p  0.05 between groups 1 and 2 in terms of NO, MDA, GSH, or planimetric analyses. Group 2 had a lower neutrophil count than group 1, however the difference was not statistically significant p  0.05. Conclusion Edaravone is a very effective antioxidant. Nevertheless, our research indicates that it may not influence IR damage in a skin flap model.

 Amaç İskemi-reperfüzyon IR hasarı, iskemik fazdan sonra kan dokuya geri döndüğünde meydana gelen doku hasarıdır. Reperfüzyon, hücre ölümü, doku bozukluğu ve nekroz ile sonuçlanan bir dizi akut inflamatuar süreç başlatır. Edaravone 3-meth-1-pheny1-2-pyrazolin-5-one güçlü ve benzersiz bir sentetik radikal temizleyicidir. Bu araştırmada, edaravonun İR hasarı üzerindeki etkileri araştırıldı. Gereç ve Yöntemler: 16 yetişkin erkek Sprague-Dawley sıçanı kullanıldı. Sekiz sıçan rastgele iki gruba ayrıldı. Grup 1 kontrol grubu, tedavi olmaksızın 10 saat boyunca karın derisi flebine iskemi ve reperfüzyonu uygulandı. Grup 2ye tedavi grubu iskemiden 10 saat önce intraperitoneal 3 mgkg edaravon enjeksiyonu ve karın derisi flebinde reperfüzyon uygulandı. Planimetri kullanılarak flepler 1, 3, 7 ve 10 günlük aralıklarla incelendi. Daha sonra biyokimyasal doku glutatyonu GSH, doku proteini, lipid peroksidasyonu MDA ve nitrik oksit NO düzeylerinin ölçümü ve histopatolojik doku glutatyonu GSH, doku proteini, lipid peroksidasyonu ölçümü için flepler çıkarıldı. MDA ve nitrik oksit NO seviyeleri Bulgular: Grup 1 ve 2 arasında NO, MDA, GSH veya planimetrik analizler açısından anlamlı bir değişiklik p  0.05 bulamadık. Grup 2, grup 1den daha düşük nötrofil sayısına sahipti, ancak fark istatistiksel olarak anlamlı değildi p  0.05. Sonuç Edaravone çok etkili bir antioksidandır. Yine de araştırmamız, bir deri flep modelinde IR hasarını etkilemeyebileceğini gösteriyor.

Article Details

Preventive Effect of Edaravone Against Ischemia-Reperfusion Injury in Rat Epigastric Island Flaps: An Experimental Study

Authors:

Ovunc Akdemir | Burak Ergün Tatar | Yiğit Uyanıkgil | Oytun Erbaş | Feng Zhang | William C. Lineaweaver

Publisher:

Selcuk University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Genel Tıp Dergisi Vol.33 Issue 2, pp.117-122

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001288864

pISSN:

2602-3741

epigastric flap

edaravone

ischemia reperfusion injuryedaravon

iskemi reperfüzyon hasarı

epigastrik flap

Issues

Article Details

Preventive Effect of Edaravone Against Ischemia-Reperfusion Injury in Rat Epigastric Island Flaps: An Experimental Study

Authors: Ovunc Akdemir | Burak Ergün Tatar | Yiğit Uyanıkgil | Oytun Erbaş | Feng Zhang | William C. Lineaweaver

Publisher: Selcuk University

Main Topics: Klinik Tıp Bilimleri

Journal: Genel Tıp Dergisi Vol.33 Issue 2, pp.117-122

Document Type: Article

Publication Date: 4/2023

Document Language: English

Document ID (Org No.): 2001288864

pISSN: 2602-3741

epigastric flap

edaravone

ischemia reperfusion injuryedaravon

iskemi reperfüzyon hasarı

epigastrik flap

Issues

Authors:

Ovunc Akdemir | Burak Ergün Tatar | Yiğit Uyanıkgil | Oytun Erbaş | Feng Zhang | William C. Lineaweaver

Publisher:

Selcuk University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Genel Tıp Dergisi Vol.33 Issue 2, pp.117-122

Document Type:

Article

Publication Date:

4/2023

Document Language:

English

Document ID (Org No.):

2001288864

pISSN:

2602-3741