Hypoxia-Inducible Factor 1&alpha; Expression in Chorionic Tissue and Decidua of Women with Spontaneous Abortion at the First Trimester of Pregnancy

E. V. Mashkina; K. A. Kovalenko; E. V. Butenko

doi:10.3844/ajbbsp.2014.275.280

Article Details

Hypoxia-Inducible Factor 1α Expression in Chorionic Tissue and Decidua of Women with Spontaneous Abortion at the First Trimester of Pregnancy

Authors:

E. V. Mashkina | K. A. Kovalenko | E. V. Butenko

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.10 Issue 4, pp.275-280

Document Type:

Article

Publication Date:

11/2014

Document Language:

English

Document ID (Org No.):

2001397193

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2014.275.280

Keywords:

Abstract:

 Oxygen-regulated genes expression has important role in pre-implantation embryonic metabolism regulation. Hypoxia Inducible Factor (HIF) regulated by hypoxia oxygen tension is crucial for placenta development. But the data about its role in spontaneous abortion is very poor. Thus, we aimed to determine an expression level of HIF-1α in chorionic tissue and decidua at pregnancy. Samples of chorionic tissue and decidua were taken after surgical termination of normally progressing pregnancies in 5-9 week of gestation (n = 8) and spontaneous abortion in 5-9 week of gestation (n = 9). HIF-1α expression was analyzed using semi-quantitative reverse transcription-polymerase chain reaction. Compared with decidual tissue, the expression of HIF-1α was increased in chorionic tissue in condition of normally progressing pregnancy. HIF-1α expression in samples of both tissues is equal in spontaneous abortion. In same time the expression of HIF-1α was decreased (1,5 fold) in chorionic tissue for spontaneous abortion compared with control group. The results demonstrated that low HIF-1α expression level in chorionic tissue can be associated with spontaneous abortion in first trimester of pregnancy.

Article Details

Hypoxia-Inducible Factor 1α Expression in Chorionic Tissue and Decidua of Women with Spontaneous Abortion at the First Trimester of Pregnancy

Authors:

E. V. Mashkina | K. A. Kovalenko | E. V. Butenko

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.10 Issue 4, pp.275-280

Document Type:

Article

Publication Date:

11/2014

Document Language:

English

Document ID (Org No.):

2001397193

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2014.275.280

Spontaneous Abortion

HIF-1

Gene Expression

Chorionic Tissue

Issues

Article Details

Hypoxia-Inducible Factor 1&alpha; Expression in Chorionic Tissue and Decidua of Women with Spontaneous Abortion at the First Trimester of Pregnancy

Authors: E. V. Mashkina | K. A. Kovalenko | E. V. Butenko

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.10 Issue 4, pp.275-280

Document Type: Article

Publication Date: 11/2014

Document Language: English

Document ID (Org No.): 2001397193

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2014.275.280

Spontaneous Abortion

HIF-1

Gene Expression

Chorionic Tissue

Issues

Hypoxia-Inducible Factor 1α Expression in Chorionic Tissue and Decidua of Women with Spontaneous Abortion at the First Trimester of Pregnancy

Authors:

E. V. Mashkina | K. A. Kovalenko | E. V. Butenko

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.10 Issue 4, pp.275-280

Document Type:

Article

Publication Date:

11/2014

Document Language:

English

Document ID (Org No.):

2001397193

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2014.275.280