Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris

Murat Ozturk; Ali Endes

Article Details

Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris

Authors:

Murat Ozturk | Ali Endes

Publisher:

Harran University

Main Topics:

Bitki Bilimi | Ziraat Mühendisliği

Journal:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.27 Issue 1, pp.64-72

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001285497

pISSN:

2587-1358

Keywords:

Abstract:

 In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates YBUFoc9 and YBUFoc2 of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens BM23 and BM40, 3 isolates as B. subtilis BM8, BM32, and BM105, 6 isolates as B. cereus BM10, BM69, BM70, BM104, BM111 and BM215, 2 isolates as B. megaterium BM44 and BM135 and 1 isolate as B. pumilus BM28. No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50 and from 0 to 58.33, respectively. Among the 5 different species, B. amyloliquefaciens BM23 and BM40 and B. subtilis BM8, BM32, and BM105 showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates BM23, BM40, BM8, BM32 and BM105 will be investigated and compared with approved fungicides for their fungicidal activity.

 In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates YBUFoc9 and YBUFoc2 of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens BM23 and BM40, 3 isolates as B. subtilis BM8, BM32, and BM105, 6 isolates as B. cereus BM10, BM69, BM70, BM104, BM111 and BM215, 2 isolates as B. megaterium BM44 and BM135 and 1 isolate as B. pumilus BM28. No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50 and from 0 to 58.33, respectively. Among the 5 different species, B. amyloliquefaciens BM23 and BM40 and B. subtilis BM8, BM32, and BM105 showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates BM23, BM40, BM8, BM32 and BM105 will be investigated and compared with approved fungicides for their fungicidal activity.

 In 2021, post-harvest soil samples were taken from fields where chickpea and wheat were grown in Yozgat province to isolate bacteria. Of 74 isolates obtained from soil samples, 14 nonpathogenic isolates were evaluated for their inhibitory potential against two pathogenic isolates YBUFoc9 and YBUFoc2 of Fusarium wilt disease agent Fusarium oxysporum f. sp. ciceris. Antagonistic bacterial isolates were identified as Bacillus spp. by Blastn analysis based on their 16S rDNA nucleotide sequences. Among them, 2 isolates were identified as B. amyloliquefaciens BM23 and BM40, 3 isolates as B. subtilis BM8, BM32, and BM105, 6 isolates as B. cereus BM10, BM69, BM70, BM104, BM111 and BM215, 2 isolates as B. megaterium BM44 and BM135 and 1 isolate as B. pumilus BM28. No effective isolate of any genus other than Bacillus spp. was found. They inhibited mycelial growth of isolates YBUFoc9 and YBUFoc2 from 0 to 78.50 and from 0 to 58.33, respectively. Among the 5 different species, B. amyloliquefaciens BM23 and BM40 and B. subtilis BM8, BM32, and BM105 showed the strongest inhibitory activity against the two fungal isolates under in vitro conditions. In further studies, the biocontrol properties of the most effective isolates BM23, BM40, BM8, BM32 and BM105 will be investigated and compared with approved fungicides for their fungicidal activity.

 2021 yılında Yozgat ilinde nohut ve buğday yetiştirilen tarlalardan bakteri izolasyonu için hasat sonrası toprak örnekleri toplanmıştır. Toprak örneklerinden izole edilen 74 izolat arasından patojenik olmayan 14 aday bakteri izolatın Fusarium solgunluğu etmeni Fusarium oxysporum f. sp. cicerisin iki izolatına YBUFoc9 and YBUFoc2 karşı engelleme potansiyelleri in vitro koşullarda değerlendirilmiştir. Antagonist bakteri izolatların tanısı 16S rDNA nükleotid dizilerine dayanan Blastn analizi ile Bacillus spp. olarak belirlenmiştir. Elde edilen izolatların 2si B. amyloliquefaciens BM23 ve BM40, 3ü B. subtilis BM8, BM32 ve BM105, 6sı B. cereus BM10, BM69, BM70, BM104, BM111 ve BM215, 2si B. megaterium olarak BM44 ve BM135 ve 1i B. pumilus BM28 olarak tanılanmıştır. Antagonistik etkinlik bakımından Bacillus spp. dışında etkili bir cinse rastlanılmamıştır. Bakteriyel izolatlar, YBUFoc9 ve YBUFoc2 izolatlarının misel büyümesine sırasıyla 0-78.50 ve 0-58.33 oranında inhibisyon sağlamışlardır. Beş farklı tür arasından B. amyloliquefaciens BM23 ve BM40 ve B. subtilisin BM8, BM32 ve BM105 in vitro koşullar altında patojenin iki izolatına karşı en güçlü inhibisyon aktivitesi gösterdiği belirlenmiştir. Gelecekte yapılması planlanan çalışmalarda, en etkili izolatların BM23, BM40, BM8, BM32 ve BM105 biyokontrol özellikleri araştırılacak ve fungisidal aktiviteleri için onaylanmış fungisitlerle karşılaştırılacaktır.

Article Details

Isolation of bacterial isolates from soil samples and determination of their in vitro antagonistic potential against chickpea wilt disease agent Fusarium oxysporum f. sp. ciceris

Authors:

Murat Ozturk | Ali Endes

Publisher:

Harran University

Main Topics:

Bitki Bilimi | Ziraat Mühendisliği

Journal:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.27 Issue 1, pp.64-72

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001285497

pISSN:

2587-1358

Fusarium wilt

Chickpea

Bacillus spp.

Biological control

Fusarium solgunluğu

Nohut

Bacillus spp.

Biyolojik mücadele

Issues

Article Details

Authors: Murat Ozturk | Ali Endes

Publisher: Harran University

Main Topics: Bitki Bilimi | Ziraat Mühendisliği

Journal: Harran Tarım ve Gıda Bilimleri Dergisi Vol.27 Issue 1, pp.64-72

Document Type: Article

Publication Date: 3/2023

Document Language: English

Document ID (Org No.): 2001285497

pISSN: 2587-1358

Issues

Authors:

Murat Ozturk | Ali Endes

Publisher:

Harran University

Main Topics:

Bitki Bilimi | Ziraat Mühendisliği

Journal:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.27 Issue 1, pp.64-72

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001285497

pISSN:

2587-1358