Phylogenetic characterization of two common sandflies, Phlebotomus major and P. kandelakii, in Inebolu District of the West Black Sea Region, Türkiye based on mitochondrial gene sequence analysis

Gupse Kübra Karademir; Mübeccel Atelge; Kardelen Yetişmiş; Gamze Yetişmiş; Sadullah Uslu; Arif Çiloğlu; Zuhal Önder; Yusuf Özbel; Gökmen Zafer Pekmezci; Alparslan Yıldırım; Önder Düzlü; Seray Töz; Didem Pekmezci; Abdullah İnci

Article Details

Phylogenetic characterization of two common sandflies, Phlebotomus major and P. kandelakii, in Inebolu District of the West Black Sea Region, Türkiye based on mitochondrial gene sequence analysis

Authors:

Gupse Kübra Karademir | Mübeccel Atelge | Kardelen Yetişmiş | Gamze Yetişmiş | Sadullah Uslu | Arif Çiloğlu | Zuhal Önder | Yusuf Özbel | Gökmen Zafer Pekmezci | Alparslan Yıldırım | Önder Düzlü | Seray Töz | Didem Pekmezci | Abdullah İnci

Publisher:

Ankara University

Main Topics:

Veterinary Epidemiology | Veterinary Parasitology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.427-436

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001325156

pISSN:

1300-0861

Keywords:

Abstract:

 Phlebotomus major and P. kandelakii are members of the Larroussius subgenus, which includes important vector sand fly species. Most members of the subgenus Larroussius have the ability to transmit Leishmania infantum, the causative agent of visceral leishmaniasis. Here, we investigated the genetic diversity within each species collected from the West Black Sea Region of Türkiye using mitochondrial DNA markers, specifically cytochrome oxidase I COI and cytochrome b gene sequences Cytb. A total of 1889 sand fly specimens were collected from the study area in June 2021 and August 2022; 1596 84.49 were identified as P. major sensu lato, and 253 13.40 were identified as P. kandelakii. Nine and four haplotypes of P. major were determined in the study area based on COI and Cytb sequences, respectively. Analysis of the phylogenetic datasets generated from our isolates and published isolates in GenBank revealed high haplotype diversities within P. major COI  0.933, Cytb  0.714. For P. kandelakii, we detected four and three haplotypes within the COI and Cytb sequences, and the haplotype diversities were also high in the datasets, including our isolates and published isolates in GenBank COI  0.978, Cytb  1.000. Pairwise mean genetic distances calculated from the COI and Cytb datasets were 0.4 and 1.4 for P. major and 1.0 and 0.2 for P. kandelakii, respectively, suggesting the absence of cryptic species. Phylogenetic analyses revealed three and two major clusters of the Larroussius subgenus in the COI and Cytb datasets, respectively. Our study contributes to molecular information for P. major and P. kandelakii distributed in Türkiye and provides valuable insights into the phylogenetic relationships among species within the subgenus Larroussius.

 Phlebotomus major and P. kandelakii are members of the Larroussius subgenus, which includes important vector sand fly species. Most members of the subgenus Larroussius have the ability to transmit Leishmania infantum, the causative agent of visceral leishmaniasis. Here, we investigated the genetic diversity within each species collected from the West Black Sea Region of Türkiye using mitochondrial DNA markers, specifically cytochrome oxidase I COI and cytochrome b gene sequences Cytb. A total of 1889 sand fly specimens were collected from the study area in June 2021 and August 2022; 1596 84.49 were identified as P. major sensu lato, and 253 13.40 were identified as P. kandelakii. Nine and four haplotypes of P. major were determined in the study area based on COI and Cytb sequences, respectively. Analysis of the phylogenetic datasets generated from our isolates and published isolates in GenBank revealed high haplotype diversities within P. major COI  0.933, Cytb  0.714. For P. kandelakii, we detected four and three haplotypes within the COI and Cytb sequences, and the haplotype diversities were also high in the datasets, including our isolates and published isolates in GenBank COI  0.978, Cytb  1.000. Pairwise mean genetic distances calculated from the COI and Cytb datasets were 0.4 and 1.4 for P. major and 1.0 and 0.2 for P. kandelakii, respectively, suggesting the absence of cryptic species. Phylogenetic analyses revealed three and two major clusters of the Larroussius subgenus in the COI and Cytb datasets, respectively. Our study contributes to molecular information for P. major and P. kandelakii distributed in Türkiye and provides valuable insights into the phylogenetic relationships among species within the subgenus Larroussius.

 Phlebotomus major and P. kandelakii are members of the Larroussius subgenus, which includes important vector sand fly species. Most members of the subgenus Larroussius have the ability to transmit Leishmania infantum, the causative agent of visceral leishmaniasis. Here, we investigated the genetic diversity within each species collected from the West Black Sea Region of Türkiye using mitochondrial DNA markers, specifically cytochrome oxidase I COI and cytochrome b gene sequences Cytb. A total of 1889 sand fly specimens were collected from the study area in June 2021 and August 2022; 1596 84.49 were identified as P. major sensu lato, and 253 13.40 were identified as P. kandelakii. Nine and four haplotypes of P. major were determined in the study area based on COI and Cytb sequences, respectively. Analysis of the phylogenetic datasets generated from our isolates and published isolates in GenBank revealed high haplotype diversities within P. major COI  0.933, Cytb  0.714. For P. kandelakii, we detected four and three haplotypes within the COI and Cytb sequences, and the haplotype diversities were also high in the datasets, including our isolates and published isolates in GenBank COI  0.978, Cytb  1.000. Pairwise mean genetic distances calculated from the COI and Cytb datasets were 0.4 and 1.4 for P. major and 1.0 and 0.2 for P. kandelakii, respectively, suggesting the absence of cryptic species. Phylogenetic analyses revealed three and two major clusters of the Larroussius subgenus in the COI and Cytb datasets, respectively. Our study contributes to molecular information for P. major and P. kandelakii distributed in Türkiye and provides valuable insights into the phylogenetic relationships among species within the subgenus Larroussius.

 Phlebotomus major and P. kandelakii are members of the Larroussius subgenus, which includes important vector sand fly species. Most members of the subgenus Larroussius have the ability to transmit Leishmania infantum, the causative agent of visceral leishmaniasis. Here, we investigated the genetic diversity within each species collected from the West Black Sea Region of Türkiye using mitochondrial DNA markers, specifically cytochrome oxidase I COI and cytochrome b gene sequences Cytb. A total of 1889 sand fly specimens were collected from the study area in June 2021 and August 2022; 1596 84.49 were identified as P. major sensu lato, and 253 13.40 were identified as P. kandelakii. Nine and four haplotypes of P. major were determined in the study area based on COI and Cytb sequences, respectively. Analysis of the phylogenetic datasets generated from our isolates and published isolates in GenBank revealed high haplotype diversities within P. major COI  0.933, Cytb  0.714. For P. kandelakii, we detected four and three haplotypes within the COI and Cytb sequences, and the haplotype diversities were also high in the datasets, including our isolates and published isolates in GenBank COI  0.978, Cytb  1.000. Pairwise mean genetic distances calculated from the COI and Cytb datasets were 0.4 and 1.4 for P. major and 1.0 and 0.2 for P. kandelakii, respectively, suggesting the absence of cryptic species. Phylogenetic analyses revealed three and two major clusters of the Larroussius subgenus in the COI and Cytb datasets, respectively. Our study contributes to molecular information for P. major and P. kandelakii distributed in Türkiye and provides valuable insights into the phylogenetic relationships among species within the subgenus Larroussius.

Article Details

Phylogenetic characterization of two common sandflies, Phlebotomus major and P. kandelakii, in Inebolu District of the West Black Sea Region, Türkiye based on mitochondrial gene sequence analysis

Authors:

Gupse Kübra Karademir | Mübeccel Atelge | Kardelen Yetişmiş | Gamze Yetişmiş | Sadullah Uslu | Arif Çiloğlu | Zuhal Önder | Yusuf Özbel | Gökmen Zafer Pekmezci | Alparslan Yıldırım | Önder Düzlü | Seray Töz | Didem Pekmezci | Abdullah İnci

Publisher:

Ankara University

Main Topics:

Veterinary Epidemiology | Veterinary Parasitology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.427-436

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001325156

pISSN:

1300-0861

COI

Cytb

haplotype

Sand fly

West Black Sea Region

Issues

Article Details

Phylogenetic characterization of two common sandflies, Phlebotomus major and P. kandelakii, in Inebolu District of the West Black Sea Region, Türkiye based on mitochondrial gene sequence analysis

Authors: Gupse Kübra Karademir | Mübeccel Atelge | Kardelen Yetişmiş | Gamze Yetişmiş | Sadullah Uslu | Arif Çiloğlu | Zuhal Önder | Yusuf Özbel | Gökmen Zafer Pekmezci | Alparslan Yıldırım | Önder Düzlü | Seray Töz | Didem Pekmezci | Abdullah İnci

Publisher: Ankara University

Main Topics: Veterinary Epidemiology | Veterinary Parasitology

Journal: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.427-436

Document Type: Article

Publication Date: 9/2024

Document Language: English

Document ID (Org No.): 2001325156

pISSN: 1300-0861

COI

Cytb

haplotype

Sand fly

West Black Sea Region

Issues

Authors:

Gupse Kübra Karademir | Mübeccel Atelge | Kardelen Yetişmiş | Gamze Yetişmiş | Sadullah Uslu | Arif Çiloğlu | Zuhal Önder | Yusuf Özbel | Gökmen Zafer Pekmezci | Alparslan Yıldırım | Önder Düzlü | Seray Töz | Didem Pekmezci | Abdullah İnci

Publisher:

Ankara University

Main Topics:

Veterinary Epidemiology | Veterinary Parasitology

Journal:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.71 Issue 4, pp.427-436

Document Type:

Article

Publication Date:

9/2024

Document Language:

English

Document ID (Org No.):

2001325156

pISSN:

1300-0861