Preliminary Study on the Genetic Diversity of Hepatozoon canis in Dogs and Rhipicephalus sanguineus sensu lato Ticks

Sezayi Özübek

Article Details

Preliminary Study on the Genetic Diversity of Hepatozoon canis in Dogs and Rhipicephalus sanguineus sensu lato Ticks

Authors:

Sezayi Özübek

Publisher:

Dicle University

Main Topics:

Veteriner Parazitoloji

Journal:

Dicle Üniversitesi Veteriner Fakültesi Dergisi Vol.17 Issue 2, pp.118-123

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001335552

pISSN:

1307-9972

Keywords:

Abstract:

 Hepatozoon canis, a protozoan parasite, is the primary cause of canine hepatozoonosis worldwide, typically causing subclinical infection in dogs but potentially leading to severe illness when accompanied by other pathogens. This study investigates the genetic diversity of H. canis in dogs and Rhipicephalus sanguineus sensu lato ticks using bioinformatics analysis. Archived DNA samples from dogs and ticks were analyzed through PCR amplification of the 18S rRNA gene, followed by sequence comparison using BLAST analysis and phylogenetic analysis using bioinformatics tools. The results revealed genetic variability, identifying several single nucleotide polymorphisms SNPs critical for distinguishing between different haplotypes. Minimum Spanning Networks created in PopART identified 18 distinct haplotypes across a broad geographical distribution. The study highlights the extensive genetic diversity of H. canis, with implications for understanding its evolutionary dynamics, transmission, pathogenicity, and resistance. Future studies should employ more variable genomic regions to further elucidate the genetic landscape of H. canis, aiding in the development of targeted control strategies and enhancing epidemiological knowledge.

 Hepatozoon canis, a protozoan parasite, is the primary cause of canine hepatozoonosis worldwide, typically causing subclinical infection in dogs but potentially leading to severe illness when accompanied by other pathogens. This study investigates the genetic diversity of H. canis in dogs and Rhipicephalus sanguineus sensu lato ticks using bioinformatics analysis. Archived DNA samples from dogs and ticks were analyzed through PCR amplification of the 18S rRNA gene, followed by sequence comparison using BLAST analysis and phylogenetic analysis using bioinformatics tools. The results revealed genetic variability, identifying several single nucleotide polymorphisms SNPs critical for distinguishing between different haplotypes. Minimum Spanning Networks created in PopART identified 18 distinct haplotypes across a broad geographical distribution. The study highlights the extensive genetic diversity of H. canis, with implications for understanding its evolutionary dynamics, transmission, pathogenicity, and resistance. Future studies should employ more variable genomic regions to further elucidate the genetic landscape of H. canis, aiding in the development of targeted control strategies and enhancing epidemiological knowledge.

 Hepatozoon canis, a protozoan parasite, is the primary cause of canine hepatozoonosis worldwide, typically causing subclinical infection in dogs but potentially leading to severe illness when accompanied by other pathogens. This study investigates the genetic diversity of H. canis in dogs and Rhipicephalus sanguineus sensu lato ticks using bioinformatics analysis. Archived DNA samples from dogs and ticks were analyzed through PCR amplification of the 18S rRNA gene, followed by sequence comparison using BLAST analysis and phylogenetic analysis using bioinformatics tools. The results revealed genetic variability, identifying several single nucleotide polymorphisms SNPs critical for distinguishing between different haplotypes. Minimum Spanning Networks created in PopART identified 18 distinct haplotypes across a broad geographical distribution. The study highlights the extensive genetic diversity of H. canis, with implications for understanding its evolutionary dynamics, transmission, pathogenicity, and resistance. Future studies should employ more variable genomic regions to further elucidate the genetic landscape of H. canis, aiding in the development of targeted control strategies and enhancing epidemiological knowledge.

 Hepatozoon canis, köpeklerde tipik olarak subklinik enfeksiyona neden olan ancak diğer patojenlerle birlikte olduğunda ciddi hastalıklara yol açabilen bir protozoon paraziti, dünya çapında köpeklerde hepatozoonozun birincil sebebidir. Bu çalışma, köpeklerde ve Rhipicephalus sanguineus sensu lato kenelerinde H. canisin genetik çeşitliliğini biyoinformatik analiz kullanarak araştırmaktadır. Köpeklerden ve kenelerden alınan arşiv DNA örnekleri, 18S rRNA geninin PCR amplifikasyonu ile analiz edilmiş ve ardından BLAST analizi ile dizi karşılaştırması ve biyoinformatik araçlar kullanılarak filogenetik analiz yapılmıştır. Sonuçlar, farklı haplotipleri ayırt etmek için kritik olan çeşitli tek nükleotid polimorfizmlerini SNPler tanımlayarak genetik değişkenliği ortaya koymuştur. PopARTda oluşturulan Minimum Yayılma Ağları, geniş bir coğrafi dağılıma sahip 18 farklı haplotipi belirlemiştir. Çalışma, H. canisin geniş genetik çeşitliliğini vurgulamakta ve evrimsel dinamiklerini, bulaşma yollarını, patojenitesini ve direncini anlamak için önemli çıkarımlarda bulunmaktadır. Gelecekteki çalışmalar, H. canisin genetik yapısını daha ayrıntılı bir şekilde açıklığa kavuşturmak, hedeflenmiş kontrol stratejilerinin geliştirilmesine ve epidemiyolojik bilginin artırılmasına yardımcı olmak için daha değişken genom bölgelerini kullanmalıdır.

Article Details

Preliminary Study on the Genetic Diversity of Hepatozoon canis in Dogs and Rhipicephalus sanguineus sensu lato Ticks

Authors:

Sezayi Özübek

Publisher:

Dicle University

Main Topics:

Veteriner Parazitoloji

Journal:

Dicle Üniversitesi Veteriner Fakültesi Dergisi Vol.17 Issue 2, pp.118-123

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001335552

pISSN:

1307-9972

Bioinformatics

genetic diversity

haplotype

Hepatozoon canis

Rhipicephalus sanguineus sensu lato: Köpek

genetik çeşitlilik

Hepatozoon canis

Rhipicephalus sanguineus sensu lato

18S rRNA

Issues

Article Details

Authors: Sezayi Özübek

Publisher: Dicle University

Main Topics: Veteriner Parazitoloji

Journal: Dicle Üniversitesi Veteriner Fakültesi Dergisi Vol.17 Issue 2, pp.118-123

Document Type: Article

Publication Date: 12/2024

Document Language: English

Document ID (Org No.): 2001335552

pISSN: 1307-9972

Issues

Authors:

Sezayi Özübek

Publisher:

Dicle University

Main Topics:

Veteriner Parazitoloji

Journal:

Dicle Üniversitesi Veteriner Fakültesi Dergisi Vol.17 Issue 2, pp.118-123

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001335552

pISSN:

1307-9972