Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin

Ayşe Ceyhan; Münevver Baran; Pınar Alişan Suna; Özge Cengiz Mat; Arzu Yay

Article Details

Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin

Authors:

Ayşe Ceyhan | Münevver Baran | Pınar Alişan Suna | Özge Cengiz Mat | Arzu Yay

Publisher:

Cukurova University

Main Topics:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Journal:

Cukurova Medical Journal Vol.48 Issue 4, pp.1248-1257

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001286422

pISSN:

2602-3032

Keywords:

Abstract:

 Purpose: We aimed the potential protective effects of curcumin CUR and beta-carotene BC against cisplatin CIS-induced ovarian damage using histological and immunohistochemical methods. Materials and Methods: We used 56 female Wistar albino rats, divided randomly into seven groups. Control rats did not receive any treatment; the sham group was administered 1 mlkg sesame oil by gavage; the CIS group 5 mgkg CIS; the CUR group 200 mgkg CUR; the BC group 100 mgkg BC; the CUR  CIS group, CIS after administration of CUR; the BC  CIS group was pretreated with BC, then administered CIS. The ovaries of all groups were excised five days after the last application. We assessed histopathology and counted and classified follicles. Expression of anti-Mullerian hormone AMH and nuclear factor-kappa B NF-κB was detected immunohistochemically. Apoptosis was evaluated using the TUNEL method. Results: CUR and BC are protective against decreased numbers of primordial, primary, preantral, secondary, and tertiary follicles caused by CIS. Both Cur and BC reduced ovarian NF-κB levels in comparison to the control group, and AMH immunoreactivity was almost identical for the control and CUR  CIS groups. Apoptotic cell counts indicated that CUR exerts a stronger anti-apoptotic effect than BC. Conclusion: CUR has a protective effect against ovarian damage brought on by CIS and greater antioxidant and anti-inflammatory properties than BC.

 Purpose: We aimed the potential protective effects of curcumin CUR and beta-carotene BC against cisplatin CIS-induced ovarian damage using histological and immunohistochemical methods. Materials and Methods: We used 56 female Wistar albino rats, divided randomly into seven groups. Control rats did not receive any treatment; the sham group was administered 1 mlkg sesame oil by gavage; the CIS group 5 mgkg CIS; the CUR group 200 mgkg CUR; the BC group 100 mgkg BC; the CUR  CIS group, CIS after administration of CUR; the BC  CIS group was pretreated with BC, then administered CIS. The ovaries of all groups were excised five days after the last application. We assessed histopathology and counted and classified follicles. Expression of anti-Mullerian hormone AMH and nuclear factor-kappa B NF-κB was detected immunohistochemically. Apoptosis was evaluated using the TUNEL method. Results: CUR and BC are protective against decreased numbers of primordial, primary, preantral, secondary, and tertiary follicles caused by CIS. Both Cur and BC reduced ovarian NF-κB levels in comparison to the control group, and AMH immunoreactivity was almost identical for the control and CUR  CIS groups. Apoptotic cell counts indicated that CUR exerts a stronger anti-apoptotic effect than BC. Conclusion: CUR has a protective effect against ovarian damage brought on by CIS and greater antioxidant and anti-inflammatory properties than BC.

 Purpose: We aimed the potential protective effects of curcumin CUR and beta-carotene BC against cisplatin CIS-induced ovarian damage using histological and immunohistochemical methods. Materials and Methods: We used 56 female Wistar albino rats, divided randomly into seven groups. Control rats did not receive any treatment; the sham group was administered 1 mlkg sesame oil by gavage; the CIS group 5 mgkg CIS; the CUR group 200 mgkg CUR; the BC group 100 mgkg BC; the CUR  CIS group, CIS after administration of CUR; the BC  CIS group was pretreated with BC, then administered CIS. The ovaries of all groups were excised five days after the last application. We assessed histopathology and counted and classified follicles. Expression of anti-Mullerian hormone AMH and nuclear factor-kappa B NF-κB was detected immunohistochemically. Apoptosis was evaluated using the TUNEL method. Results: CUR and BC are protective against decreased numbers of primordial, primary, preantral, secondary, and tertiary follicles caused by CIS. Both Cur and BC reduced ovarian NF-κB levels in comparison to the control group, and AMH immunoreactivity was almost identical for the control and CUR  CIS groups. Apoptotic cell counts indicated that CUR exerts a stronger anti-apoptotic effect than BC. Conclusion: CUR has a protective effect against ovarian damage brought on by CIS and greater antioxidant and anti-inflammatory properties than BC.

 Amaç: Cisplatin CIS kaynaklı over hasarına karşı kurkumin CUR ve beta-karotenin BC potansiyel koruyucu etkilerinin histolojik ve immünohistokimyasal yöntemlerle araştırılması amaçlanmıştır. Gereç ve Yöntem: Çalışmada, rastgele yedi gruba ayrılan 56 dişi Wistar albino sıçan kullanıldı. Kontrol sıçanları herhangi bir tedavi görmedi; sham grubuna gavajla 1 mlkg susam yağı verildi; CIS grubuna 5 mgkg CIS; CUR grubuna 200 mgkg CUR; BC grubuna 200 mgkg BC; CUR  CIS grubuna, CUR uygulamasından sonra CIS uygulandı; BC  CIS grubu, BC ile ön işleme tabi tutuldu, ardından CIS uygulandı. Tüm grupların overleri son uygulamadan beş gün sonra eksize edildi. Elde edilen dokularda, histopatolojik değerlendirme yapıldı ve folikül rezervi hesaplandı. Anti-Mullerian hormon AMH ve nükleer faktör-kappa B NF-κB ekspresyonu immünohistokimyasal olarak saptandı. Apoptoz ise, TUNEL yöntemi kullanılarak değerlendirildi. Bulgular: CUR ve BCnin, CISin neden olduğu azalan primordial, primer, preantral, sekonder ve tersiyer folikül sayılarına karşı koruyucu olduğu gözlendi. AMH immünoreaktivitesi, kontrol ve CUR  CIS grupları için hemen hemen aynıydı. CUR ve BC gruplarında kontrollere kıyasla over NF-κB ekspresyon seviyelerini düşürdü. TUNEL pozitif apoptotik hücre sayıları, CURnin BCden daha güçlü bir anti-apoptotik etki gösterdiğini gösterdi. Sonuç: CISnin neden olduğu over hasarına karşı CURnin BCden daha fazla antioksidan ve antiinflamatuar etkiye sahip olduğunu gösterilmiştir.

Article Details

Comparison of effects of curcumin and beta-carotene on ovarian damage caused by cisplatin

Authors:

Ayşe Ceyhan | Münevver Baran | Pınar Alişan Suna | Özge Cengiz Mat | Arzu Yay

Publisher:

Cukurova University

Main Topics:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Journal:

Cukurova Medical Journal Vol.48 Issue 4, pp.1248-1257

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001286422

pISSN:

2602-3032

Anti-Mullerian hormon

Apoptosis

beta-karoten

cisplatin

kurkumin

NF-κB

Anti-Mullerian hormone

apoptosis

beta-karoten

cisplatin

kurkumin

NF-κB

Issues

Article Details

Authors: Ayşe Ceyhan | Münevver Baran | Pınar Alişan Suna | Özge Cengiz Mat | Arzu Yay

Publisher: Cukurova University

Main Topics: Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Journal: Cukurova Medical Journal Vol.48 Issue 4, pp.1248-1257

Document Type: Article

Publication Date: 12/2023

Document Language: English

Document ID (Org No.): 2001286422

pISSN: 2602-3032

Issues

Authors:

Ayşe Ceyhan | Münevver Baran | Pınar Alişan Suna | Özge Cengiz Mat | Arzu Yay

Publisher:

Cukurova University

Main Topics:

Kanser Tedavisi (Kemoterapi ve Radyoterapi hariç)

Journal:

Cukurova Medical Journal Vol.48 Issue 4, pp.1248-1257

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001286422

pISSN:

2602-3032