EXPRESSIONS OF APOPTOSIS-RELATED PROTEINS IN COLON CANCER CELLS AFTER HYP-INDUCED PHOTOTHERAPY

Aysun Kılıç SÜLOĞLU; Elif KARACAOĞLU; Güldeniz SELMANOĞLU; Çağatay KARAASLAN

Article Details

EXPRESSIONS OF APOPTOSIS-RELATED PROTEINS IN COLON CANCER CELLS AFTER HYP-INDUCED PHOTOTHERAPY

Authors:

Aysun Kılıç SÜLOĞLU | Elif KARACAOĞLU | Güldeniz SELMANOĞLU | Çağatay KARAASLAN

Publisher:

Engin TUTKUN

Journal:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-191

Document Type:

Article

Publication Date:

2/2017

Document Language:

Turkish

Document ID (Org No.):

2000835377

Frequency:

Quarterly

eISSN:

2149-4711

pISSN:

2149-4711

Abstract:

 


Coloractal
cancer is the third most common cancer and is prominent death factor worlwide.
Although the main treatment is a surgery, the alternative suggested treatment
method is photodynamic therapy (PDT). Hypericin (HYP) is a well-studied
photosensitizier for PDT, however mechanism of action is still unclear. The aim
of the present study is to investigate the apoptotic cell mechanism in HT-29
and Caco-2 cells photoactivated with HYP.

In the present
study, HT-29 (grade-I) and Caco-2 (grade-II) human colon cancer cells treated
with 0.04, 0.08 or 0.15 µM HYP and irradiated with (4J/cm2) flourescent lamps.
HYP effects were evaluated for 16 and 24 h. Total protein content of cells were
measured by Bradford assay. Apoptosis-related proteins including caspase-9,
Bcl-2 and Bax expressions were investigated at the mRNA level by real-time PCR
for both cells.

Protein
concentrationdramatically decreasedin all groups HT-29 cellsafter 24 h and
Caco-2 cells. However, it increased in 0.15 µM group in Caco-2 cells. Caspase-9
were upregulated for both cells in 24 h incubation. Bcl-2 expression was not
observed in HT-29 cells, however Bcl-2 downregulated in Caco-2 cells. Bax
expression downregulated in HT-29 cells after 24 h incubation and upregulated
in Caco-2 cells after 16 h incubation.

In conclusion,
Caco-2 and HT-29 colon cancer cells response distinctly to HYP-mediated PDT in
terms of apoptosis-related proteins, activation of repair mechanisms and
resistance development.

 

This research was partially supported by the
Hacettepe University Scientific Research Projects Coordination Unit (09 01 601
011).














 

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Article Details

EXPRESSIONS OF APOPTOSIS-RELATED PROTEINS IN COLON CANCER CELLS AFTER HYP-INDUCED PHOTOTHERAPY

Authors: Aysun Kılıç SÜLOĞLU | Elif KARACAOĞLU | Güldeniz SELMANOĞLU | Çağatay KARAASLAN

Publisher: Engin TUTKUN

Journal: The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-191

Document Type: Article

Publication Date: 2/2017

Document Language: Turkish

Document ID (Org No.): 2000835377

Frequency: Quarterly

eISSN: 2149-4711

pISSN: 2149-4711

Issues

Authors:

Aysun Kılıç SÜLOĞLU | Elif KARACAOĞLU | Güldeniz SELMANOĞLU | Çağatay KARAASLAN

Publisher:

Engin TUTKUN

Journal:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-191

Document Type:

Article

Publication Date:

2/2017

Document Language:

Turkish

Document ID (Org No.):

2000835377

Frequency:

Quarterly

eISSN:

2149-4711

pISSN:

2149-4711