Can Lathyrus czeczottianus Bässler Be a New Source of Natural Antimutagenic Agents in Pharmacology? Evaluation from Mutagenic/Antimutagenic and Antimicrobial Perspectives

Mustafa Kul; Ahmet Uysal

Article Details

Can Lathyrus czeczottianus Bässler Be a New Source of Natural Antimutagenic Agents in Pharmacology? Evaluation from Mutagenic/Antimutagenic and Antimicrobial Perspectives

Authors:

Mustafa Kul | Ahmet Uysal

Publisher:

Selcuk University

Main Topics:

Plant Biochemistry | Bacteriology

Journal:

Selcuk University Journal of Science Faculty Vol.50 Issue 2, pp.35-42

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001327759

pISSN:

2458-9411

Keywords:

Abstract:

 In this study, the mutagenicityantimutagenicity and antimicrobial properties of methanol and water extracts of the aerial parts of the Lathyrus czeczottianus plant were investigated. After toxic dose determinations of the extracts were made, their mutagenic properties were evaluated by the Ames test Salmonellamicrosome. Mutagenicity studies with Salmonella typhimurium TA98 and TA100 strains were performed in the presence and absence of metabolic activation. Methanol and water extracts did not show any mutagenic effects at doses of 10000, 5000 and 1000 µgplate. Antimutagenic inhibition rates against well-known mutagenic substances were evaluated. Accordingly, methanol and water extracts inhibited the effect of 2 aminofluorene at the highest doses in the presence of the S9 mixture by 73 and 85, respectively, especially for the TA98 strain. In addition, methanol extract improved the effect of 2 aminoanthracene up to 78 in the presence of S9 for the TA100 strain and showed strong antimutagenicity. Antimicrobial activity studies were tested using the broth microdilution method against eight standard microorganisms and 14 methicillin-resistant Staphylococcus aureus isolates. Methanol and water extracts revealed low antimicrobial activity against the tested microorganisms at doses of 12.5 and 6.25 mgml. It was thought that L. czeczottianus extracts could be used as a natural chemo preventive agent against chemicals. This study is the first report on genotoxicity and antimicrobial properties on L. czeczottianus.

 In this study, the mutagenicityantimutagenicity and antimicrobial properties of methanol and water extracts of the aerial parts of the Lathyrus czeczottianus plant were investigated. After toxic dose determinations of the extracts were made, their mutagenic properties were evaluated by the Ames test Salmonellamicrosome. Mutagenicity studies with Salmonella typhimurium TA98 and TA100 strains were performed in the presence and absence of metabolic activation. Methanol and water extracts did not show any mutagenic effects at doses of 10000, 5000 and 1000 µgplate. Antimutagenic inhibition rates against well-known mutagenic substances were evaluated. Accordingly, methanol and water extracts inhibited the effect of 2 aminofluorene at the highest doses in the presence of the S9 mixture by 73 and 85, respectively, especially for the TA98 strain. In addition, methanol extract improved the effect of 2 aminoanthracene up to 78 in the presence of S9 for the TA100 strain and showed strong antimutagenicity. Antimicrobial activity studies were tested using the broth microdilution method against eight standard microorganisms and 14 methicillin-resistant Staphylococcus aureus isolates. Methanol and water extracts revealed low antimicrobial activity against the tested microorganisms at doses of 12.5 and 6.25 mgml. It was thought that L. czeczottianus extracts could be used as a natural chemo preventive agent against chemicals. This study is the first report on genotoxicity and antimicrobial properties on L. czeczottianus.

 In this study, the mutagenicityantimutagenicity and antimicrobial properties of methanol and water extracts of the aerial parts of the Lathyrus czeczottianus plant were investigated. After toxic dose determinations of the extracts were made, their mutagenic properties were evaluated by the Ames test Salmonellamicrosome. Mutagenicity studies with Salmonella typhimurium TA98 and TA100 strains were performed in the presence and absence of metabolic activation. Methanol and water extracts did not show any mutagenic effects at doses of 10000, 5000 and 1000 µgplate. Antimutagenic inhibition rates against well-known mutagenic substances were evaluated. Accordingly, methanol and water extracts inhibited the effect of 2 aminofluorene at the highest doses in the presence of the S9 mixture by 73 and 85, respectively, especially for the TA98 strain. In addition, methanol extract improved the effect of 2 aminoanthracene up to 78 in the presence of S9 for the TA100 strain and showed strong antimutagenicity. Antimicrobial activity studies were tested using the broth microdilution method against eight standard microorganisms and 14 methicillin-resistant Staphylococcus aureus isolates. Methanol and water extracts revealed low antimicrobial activity against the tested microorganisms at doses of 12.5 and 6.25 mgml. It was thought that L. czeczottianus extracts could be used as a natural chemo preventive agent against chemicals. This study is the first report on genotoxicity and antimicrobial properties on L. czeczottianus.

 Bu çalışmada, Lathyrus czeczottianus bitkisinin toprak üstü kısımlarına ait metanol ve su özütlerinin mutajeniteantimutajenite ve antimikrobiyal özellikleri araştırılmıştır. Özütlerin toksik doz belirlemeleri yapıldıktan sonra, Ames testi Salmonellamikrozom ile mutajenik özellikleri değerlendirilmiştir. Salmonella typhimurium TA98 ve TA100 suşları ile yapılan mutajenite çalışmaları metabolik aktivasyon varlığı ve yokluğunda gerçekleştirilmiştir. Metanol ve su öztüleri 10000, 5000 ve 1000 µgplak dozlarda herhangi mutajenik etki göstermemiştir. Bilinen mutajenik maddelere karşı antimutajenik inhibisyon oranları değerlendirilmiştir. Buna göre metanol ve su özütleri özellikle TA98 suşu için 2 aminoflorenin S9 karışımı varlığında etkisini sırasıyla 73 ve 85 oranlarda en yüksek dozlarda inhibe etmiştir. Ayrıca metanol özütü TA100 suşu için S9 varlığında 2 aminoantrasenin etkisini 78lere varan düzeyde iyileştirmiş ve güçlü antimutajenite göstermiştir. Antimikrobiyal aktivite çalışmaları ise sekiz standart mikroorganizma ve 14 metisilin dirençli Staphylococcus aureus izolatına karşı sıvı mikro dilüsyon yöntemiyle test edilmiştir. Metanol ve su özütleri test edilen mikroorganizmalara karşı 12.5 ve 6.25 mgml dozlarda düşük antimikrobiyal aktivite ortaya koymuştur. L. czeczottianus özütlerinin kimyasallara karşı kemopreventif doğal bir ajan olarak kullanılabileceği düşünülmüştür.

Article Details

Can Lathyrus czeczottianus Bässler Be a New Source of Natural Antimutagenic Agents in Pharmacology? Evaluation from Mutagenic/Antimutagenic and Antimicrobial Perspectives

Authors:

Mustafa Kul | Ahmet Uysal

Publisher:

Selcuk University

Main Topics:

Plant Biochemistry | Bacteriology

Journal:

Selcuk University Journal of Science Faculty Vol.50 Issue 2, pp.35-42

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001327759

pISSN:

2458-9411

Antimicrobial activity

Antimutagenicity

Ames test

Lathyrus czeczottianus

MutagenicityAntimikrobiyal aktivite

Antimutajenite

Ames test

Lathyrus czeczottianus

Mutajenite

Issues

Article Details

Authors: Mustafa Kul | Ahmet Uysal

Publisher: Selcuk University

Main Topics: Plant Biochemistry | Bacteriology

Journal: Selcuk University Journal of Science Faculty Vol.50 Issue 2, pp.35-42

Document Type: Article

Publication Date: 1/2024

Document Language: Turkish

Document ID (Org No.): 2001327759

pISSN: 2458-9411

Issues

Authors:

Mustafa Kul | Ahmet Uysal

Publisher:

Selcuk University

Main Topics:

Plant Biochemistry | Bacteriology

Journal:

Selcuk University Journal of Science Faculty Vol.50 Issue 2, pp.35-42

Document Type:

Article

Publication Date:

1/2024

Document Language:

Turkish

Document ID (Org No.):

2001327759

pISSN:

2458-9411