Effect of Smoking and Periodontitis on Adropin Level in Gingival Crevicular Fluid

Buket Özsoy; Kamile Erciyas

Article Details

Effect of Smoking and Periodontitis on Adropin Level in Gingival Crevicular Fluid

Authors:

Buket Özsoy | Kamile Erciyas

Publisher:

Süleyman Demirel University

Main Topics:

Periodontoloji

Journal:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.16 Issue 1, pp.56-65

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

2001345871

pISSN:

2146-247X

Keywords:

Abstract:

 Objectives: One of the most significant environmental risk factors, smoking, acts both locally and systemically to contribute to the pathophysiology and development of periodontal diseases. The purpose of this research is to quantify and contrast the levels of adropin in gingival crevicular fluid GCF, which have a comparable mode of action to periodontal disorders and smoking-related endothelial wall dysfunction. Materials and methods: In total, 80 participants were enrolled in this study: 40 patients with periodontitis 20 smokers and 20 non-smokers and 40 healthy control patients 20 smokers and 20 non-smokers. GCF samples were obtained, and clinical parameters plaque index, gingival index, clinical attachment level, and probing pocket depth were noted for each participant. To measure the amount of adropin, the enzyme-linked immunosorbent assay ELISA method was employed. Shapiro Wilk, Mann Whitney U, Kruskall Wallis, and Chi square tests were used to statistically analyze the clinical and biochemical parameters. Results: Periodontitis, GCF adropin concentrations and periodontal clinical index averages were higher than healthy patients p0.001. There was no statistically significant difference in GCF adropin concentrations between smokers with periodontitis PS and nonsmokers with periodontitis P, or between smokers who were periodontally healthy HS and nonsmokers who were periodontally healthy H. Conclusions Adropin may be a potential diagnostic tool for periodontitis, regardless of smoking. However, studies investigating different biochemical parameters in GCF are needed to help understand the interaction of adropin with other mediators.

 Objectives: One of the most significant environmental risk factors, smoking, acts both locally and systemically to contribute to the pathophysiology and development of periodontal diseases. The purpose of this research is to quantify and contrast the levels of adropin in gingival crevicular fluid GCF, which have a comparable mode of action to periodontal disorders and smoking-related endothelial wall dysfunction. Materials and methods: In total, 80 participants were enrolled in this study: 40 patients with periodontitis 20 smokers and 20 non-smokers and 40 healthy control patients 20 smokers and 20 non-smokers. GCF samples were obtained, and clinical parameters plaque index, gingival index, clinical attachment level, and probing pocket depth were noted for each participant. To measure the amount of adropin, the enzyme-linked immunosorbent assay ELISA method was employed. Shapiro Wilk, Mann Whitney U, Kruskall Wallis, and Chi square tests were used to statistically analyze the clinical and biochemical parameters. Results: Periodontitis, GCF adropin concentrations and periodontal clinical index averages were higher than healthy patients p0.001. There was no statistically significant difference in GCF adropin concentrations between smokers with periodontitis PS and nonsmokers with periodontitis P, or between smokers who were periodontally healthy HS and nonsmokers who were periodontally healthy H. Conclusions Adropin may be a potential diagnostic tool for periodontitis, regardless of smoking. However, studies investigating different biochemical parameters in GCF are needed to help understand the interaction of adropin with other mediators.

 Objectives: One of the most significant environmental risk factors, smoking, acts both locally and systemically to contribute to the pathophysiology and development of periodontal diseases. The purpose of this research is to quantify and contrast the levels of adropin in gingival crevicular fluid GCF, which have a comparable mode of action to periodontal disorders and smoking-related endothelial wall dysfunction. Materials and methods: In total, 80 participants were enrolled in this study: 40 patients with periodontitis 20 smokers and 20 non-smokers and 40 healthy control patients 20 smokers and 20 non-smokers. GCF samples were obtained, and clinical parameters plaque index, gingival index, clinical attachment level, and probing pocket depth were noted for each participant. To measure the amount of adropin, the enzyme-linked immunosorbent assay ELISA method was employed. Shapiro Wilk, Mann Whitney U, Kruskall Wallis, and Chi square tests were used to statistically analyze the clinical and biochemical parameters. Results: Periodontitis, GCF adropin concentrations and periodontal clinical index averages were higher than healthy patients p0.001. There was no statistically significant difference in GCF adropin concentrations between smokers with periodontitis PS and nonsmokers with periodontitis P, or between smokers who were periodontally healthy HS and nonsmokers who were periodontally healthy H. Conclusions Adropin may be a potential diagnostic tool for periodontitis, regardless of smoking. However, studies investigating different biochemical parameters in GCF are needed to help understand the interaction of adropin with other mediators.

 Amaç: En önemli çevresel risk faktörlerinden biri olan sigara, lokal ve sistemik etki göstererek periodontal hastalıkların patogenezinde ve gelişiminde rol oynamaktadır. Bu çalışmanın amacı, benzer etki mekanizmasına sahip endotel duvarında sigara içmenin ve periodontal hastalıkların neden olduğu disfonksiyonla yakından ilişkili olan diş eti oluğu sıvısındaki DOS adropin düzeylerini ölçmek ve karşılaştırmaktır. Gereç ve Yöntem: Bu çalışmaya 40 periodontitis hastası 20 sigara içen ve 20 sigara içmeyen ve 40 sağlıklı kontrol hastası 20 sigara içen ve 20 sigara içmeyen olmak üzere toplam 80 kişi dahil edildi. Tüm katılımcıların klinik ölçümleri plak indeksi, diş eti indeksi, klinik ataşman seviyesi, sondalama cep derinliği kaydedildi ve DOS örnekleri toplandı. Adropin düzeyini analiz etmek için enzime bağlı immünosorbent tahlili ELISA tekniği kullanıldı. Klinik ve biyokimyasal parametrelerin istatistiksel analizi Shapiro Wilk, Mann Whitney U, Kruskall Wallis ve Ki kare testi kullanılarak yapıldı. Bulgular: Periodontitis, DOS adropin konsantrasyonları ve periodontal klinik indeks ortalamaları sağlıklı hastalara göre daha yüksekti p0.001. Periodontitisli sigara içenler PS ile sigara içmeyen periodontitisli P veya periodontal olarak sağlıklı olan sigara içenler HS ile periodontal olarak sağlıklı olan sigara içmeyenler H arasında DOS adropin konsantrasyonları açısından istatistiksel olarak anlamlı bir fark yoktu. Sonuçlar: Adropin, sigara içimine bakılmaksızın periodontit için potansiyel bir teşhis aracı olabilir. Ancak adropinin diğer medyatörlerle etkileşiminin anlaşılmasına yardımcı olmak için DOSdaki farklı biyokimyasal parametreleri araştıran çalışmalara ihtiyaç vardır.

Article Details

Effect of Smoking and Periodontitis on Adropin Level in Gingival Crevicular Fluid

Authors:

Buket Özsoy | Kamile Erciyas

Publisher:

Süleyman Demirel University

Main Topics:

Periodontoloji

Journal:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.16 Issue 1, pp.56-65

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

2001345871

pISSN:

2146-247X

Periodontitis

Smoking

Gingival fluid

AdropinPeriodontitis

Sigara

Dişeti Oluğu Sıvısı

Adropin

Issues

Article Details

Authors: Buket Özsoy | Kamile Erciyas

Publisher: Süleyman Demirel University

Main Topics: Periodontoloji

Journal: Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.16 Issue 1, pp.56-65

Document Type: Article

Publication Date: 4/2025

Document Language: English

Document ID (Org No.): 2001345871

pISSN: 2146-247X

Issues

Authors:

Buket Özsoy | Kamile Erciyas

Publisher:

Süleyman Demirel University

Main Topics:

Periodontoloji

Journal:

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi Vol.16 Issue 1, pp.56-65

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

2001345871

pISSN:

2146-247X