Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Sevda ER; Ayşe Tansu KOPARAL; Merih KIVANÇ

Article Details

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Authors:

Sevda ER | Ayşe Tansu KOPARAL | Merih KIVANÇ

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.23-30

Document Type:

Article

Publication Date:

3/2015

Document Language:

Turkish

Document ID (Org No.):

2000812012

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

Probiotics are live microbial food supplements that can be considered a functional food. They benefit the health of a host animal by maintaining their intestinal microbial balance. Most probiotic microorganisms are lactic acid bacteria (LAB) such as Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. LAB have been reported to possess certain anticancer properties. The vast majority of studies on their anticancer effects have dealt with colorectal cancers, although there have also been some studies on breast and bladder cancers. Colon cancer is the fourth most common cause of cancer-related mortality in the world. The aim of this study was to investigate the antiproliferative effects of the cell-free filtrate and the cell-free lyophilized filtrate of 3 LAB (Pediococcus pentosaceus, Lactobacillus plantarum, and Weissella confusa) on human colorectal adenocarcinoma cell line Caco-2. The filtrates were found to inhibit the growth of colon cancer cells in a dose-dependent manner as detected by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, suggesting that these strains might have use as probiotics in functional food or for colon cancer treatment. There are no other studies related to the anticancer activities of W. confusa in the literature.

 

Probiotics are live microbial food supplements that can be considered a functional food. They benefit the health of a host animal by maintaining their intestinal microbial balance. Most probiotic microorganisms are lactic acid bacteria (LAB) such as Lactobacillus spp., Bifidobacterium spp., and Enterococcus spp. LAB have been reported to possess certain anticancer properties. The vast majority of studies on their anticancer effects have dealt with colorectal cancers, although there have also been some studies on breast and bladder cancers. Colon cancer is the fourth most common cause of cancer-related mortality in the world. The aim of this study was to investigate the antiproliferative effects of the cell-free filtrate and the cell-free lyophilized filtrate of 3 LAB (Pediococcus pentosaceus, Lactobacillus plantarum, and Weissella confusa) on human colorectal adenocarcinoma cell line Caco-2. The filtrates were found to inhibit the growth of colon cancer cells in a dose-dependent manner as detected by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, suggesting that these strains might have use as probiotics in functional food or for colon cancer treatment. There are no other studies related to the anticancer activities of W. confusa in the literature.

Article Details

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Authors:

Sevda ER | Ayşe Tansu KOPARAL | Merih KIVANÇ

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.23-30

Document Type:

Article

Publication Date:

3/2015

Document Language:

Turkish

Document ID (Org No.):

2000812012

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Probiotics

lactic acid bacteria

cell-free filtrate

Caco-2

MTT assay

Issues

Article Details

Cytotoxic effects of various lactic acid bacteria on Caco-2 cells

Authors: Sevda ER | Ayşe Tansu KOPARAL | Merih KIVANÇ

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.23-30

Document Type: Article

Publication Date: 3/2015

Document Language: Turkish

Document ID (Org No.): 2000812012

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Probiotics

lactic acid bacteria

cell-free filtrate

Caco-2

MTT assay

Issues

Authors:

Sevda ER | Ayşe Tansu KOPARAL | Merih KIVANÇ

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2015 Vol.39 Issue 1, pp.23-30

Document Type:

Article

Publication Date:

3/2015

Document Language:

Turkish

Document ID (Org No.):

2000812012

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152