Detection and Molecular Characterization of Grapevine Fanleaf Virus in Vineyards of Çanakkale Province

Ayşenur Şirin; Ali Karanfil; Savaş Korkmaz

Article Details

Detection and Molecular Characterization of Grapevine Fanleaf Virus in Vineyards of Çanakkale Province

Authors:

Ayşenur Şirin | Ali Karanfil | Savaş Korkmaz

Publisher:

Cukurova University

Main Topics:

Fitopatoloji

Journal:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 1, pp.220-230

Document Type:

Article

Publication Date:

6/2024

Document Language:

Turkish

Document ID (Org No.):

2001321321

pISSN:

2636-7874

Keywords:

Abstract:

 Diseases and pests are among the most important factors that reduce productivity and quality in vineyards. Among these, virus diseases are very important because they are difficult to combat. As a result of studies carried out in vineyard areas, many virus diseases have been identified. One of these virus diseases is grapevine fanleaf virus GFLV. Although many studies have been conducted on GFLV in Türkiye, most studies have focused on detecting the virus. This study was carried out to identify the agent and molecular characterization of its isolates. For this purpose, samples were taken from 60 plants showing virus and virus-like symptoms in the vineyard production areas of Çanakkale province and its districts and tested using reverse transcription-polymerase chain reaction RT-PCR with GFLV-specific primer pair. As a result of the tests performed, GFLV infection was detected in 34 of the samples collected. The GFLV infection rate in the collected samples was 56.66. The most striking symptom type in infected samples was determined to be yellowish chlorosis and leaf deformations on the leaves. For molecular characterization of the isolates, 4 isolates obtained from the infected samples were selected and their similarities and phylogenetic relationships among themselves and with world isolates were investigated based on the coat protein CP gene region. As a result of molecular characterization studies, it was determined that Turkish and world isolates showed 85 or more sequence similarity. As a result of phylogenetic analysis, it was determined that Turkish isolates were distributed into two different groups.

 Diseases and pests are among the most important factors that reduce productivity and quality in vineyards. Among these, virus diseases are very important because they are difficult to combat. As a result of studies carried out in vineyard areas, many virus diseases have been identified. One of these virus diseases is grapevine fanleaf virus GFLV. Although many studies have been conducted on GFLV in Türkiye, most studies have focused on detecting the virus. This study was carried out to identify the agent and molecular characterization of its isolates. For this purpose, samples were taken from 60 plants showing virus and virus-like symptoms in the vineyard production areas of Çanakkale province and its districts and tested using reverse transcription-polymerase chain reaction RT-PCR with GFLV-specific primer pair. As a result of the tests performed, GFLV infection was detected in 34 of the samples collected. The GFLV infection rate in the collected samples was 56.66. The most striking symptom type in infected samples was determined to be yellowish chlorosis and leaf deformations on the leaves. For molecular characterization of the isolates, 4 isolates obtained from the infected samples were selected and their similarities and phylogenetic relationships among themselves and with world isolates were investigated based on the coat protein CP gene region. As a result of molecular characterization studies, it was determined that Turkish and world isolates showed 85 or more sequence similarity. As a result of phylogenetic analysis, it was determined that Turkish isolates were distributed into two different groups.

 Diseases and pests are among the most important factors that reduce productivity and quality in vineyards. Among these, virus diseases are very important because they are difficult to combat. As a result of studies carried out in vineyard areas, many virus diseases have been identified. One of these virus diseases is grapevine fanleaf virus GFLV. Although many studies have been conducted on GFLV in Türkiye, most studies have focused on detecting the virus. This study was carried out to identify the agent and molecular characterization of its isolates. For this purpose, samples were taken from 60 plants showing virus and virus-like symptoms in the vineyard production areas of Çanakkale province and its districts and tested using reverse transcription-polymerase chain reaction RT-PCR with GFLV-specific primer pair. As a result of the tests performed, GFLV infection was detected in 34 of the samples collected. The GFLV infection rate in the collected samples was 56.66. The most striking symptom type in infected samples was determined to be yellowish chlorosis and leaf deformations on the leaves. For molecular characterization of the isolates, 4 isolates obtained from the infected samples were selected and their similarities and phylogenetic relationships among themselves and with world isolates were investigated based on the coat protein CP gene region. As a result of molecular characterization studies, it was determined that Turkish and world isolates showed 85 or more sequence similarity. As a result of phylogenetic analysis, it was determined that Turkish isolates were distributed into two different groups.

 Bağ alanlarında verim ve kaliteyi düşüren en önemli faktörlerin başında hastalık ve zararlılar gelmektedir. Bunların içerisinde mücadelenin zor olması nedeni ile virüs hastalıkları büyük bir öneme sahiptir. Bağ alanlarında gerçekleştirilen çalışmalar neticesinde çok sayıda virüs hastalığı tanımlanmıştır. Bu virüs hastalıklarından bir tanesi de grapevine fanleaf virus GFLVdur. GFLV ile ilgili olarak ülkemizde çok sayıda çalışma yapılmasına rağmen, çalışmaların büyük bir kısmı virüsün tespitine yönelik olmuştur. Bu çalışma etmenin tespiti ve izolatlarının moleküler karakterizasyonu amacı ile yürütülmüştür. Bu amaçla Çanakkale ili ve ilçeleri bağ üretim alanlarında virüs ve virüs benzeri simptom gösteren 60 bitkiden örnekler alınarak GFLV spesifik primer çifti ile ters transkripsiyon-polimeraz zincir reaksiyonu RT-PCR kullanılarak testlenmiştir. Gerçekleştirilen testlemeler sonucunda toplanılan örneklerin 34ünde GFLV enfeksiyonu tespit edilmiştir. Toplanılan örneklerdeki GFLV enfeksiyon oranı ise 56.66 olmuştur. Enfekteli örneklerde en dikkat çeken simptom tipi yapraklardaki sarımtrak klorozlar ve yaprak deformasyonları olarak belirlenmiştir. İzolatların moleküler karakterizasyonu amacı ile enfekteli olarak bulunan örneklerden elde edilen 4 izolat seçilerek kendi içlerinde ve dünya izolatları ile CP gen bölgesine göre göstermiş oldukları benzerlik ve filogenetik ilişkileri araştırılmıştır. Moleküler karakterizasyon çalışmaları sonucunda Türk ve dünya izolatlarının birbirleri ile 85 ve üzeri sekans benzerliği gösterdiği saptanmıştır. Filogenetik analizler sonucunda ise Türk izolatlarının iki farklı gruba dağılım gösterdiği belirlenmiştir.

Article Details

Detection and Molecular Characterization of Grapevine Fanleaf Virus in Vineyards of Çanakkale Province

Authors:

Ayşenur Şirin | Ali Karanfil | Savaş Korkmaz

Publisher:

Cukurova University

Main Topics:

Fitopatoloji

Journal:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 1, pp.220-230

Document Type:

Article

Publication Date:

6/2024

Document Language:

Turkish

Document ID (Org No.):

2001321321

pISSN:

2636-7874

GFLV

RT-PCR

Similarity

PhylogeneticGFLV

RT-PCR

Benzerlik

Filogenetik

Issues

Article Details

Authors: Ayşenur Şirin | Ali Karanfil | Savaş Korkmaz

Publisher: Cukurova University

Main Topics: Fitopatoloji

Journal: Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 1, pp.220-230

Document Type: Article

Publication Date: 6/2024

Document Language: Turkish

Document ID (Org No.): 2001321321

pISSN: 2636-7874

Issues

Authors:

Ayşenur Şirin | Ali Karanfil | Savaş Korkmaz

Publisher:

Cukurova University

Main Topics:

Fitopatoloji

Journal:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 1, pp.220-230

Document Type:

Article

Publication Date:

6/2024

Document Language:

Turkish

Document ID (Org No.):

2001321321

pISSN:

2636-7874