DETERMINATION OF THE EFFECT OF RUTIN ON EPITHHELIAL AND MESENCHIMAL TRANSITION IN PROSTATE CANCER CELLS

Esra Bal; Asuman Deveci Özkan; Zeynep Betts

Article Details

DETERMINATION OF THE EFFECT OF RUTIN ON EPITHHELIAL AND MESENCHIMAL TRANSITION IN PROSTATE CANCER CELLS

Authors:

Esra Bal | Asuman Deveci Özkan | Zeynep Betts

Publisher:

Kocaeli University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer)

Journal:

Acta Medica Nicomedia Vol.6 Issue 1, pp.131-136

Document Type:

Article

Publication Date:

2/2023

Document Language:

English

Document ID (Org No.):

2001284604

pISSN:

2717-8994

Keywords:

Abstract:

 Objective: The difficulties experienced in the treatment of prostate cancer and the excess of side effects due to chemotherapy have brought the search for alternative treatment strategies. In recent studies, it is known that Rutin RUT has an anti-cancer effect on cancer cells. Our study aimed to determine the effects of RUT on epithelial-mesenchymal transition EMT in prostate cancer cells, for the first time. Methods: The anticancer activity of RUT in prostate cancer cells PC-3 was determined by WST-1, Annexin V ELISA, DAPI and Acridine Orange staining, and the anti-cancer and anti-metastatic properties of RUT were evaluated with the Scratch Assay test. The mRNA expression level of Bax, Bcl-2, Snail, Twist, Vimentin and E-cadherin genes was determined by RT-PCR. Results: PC-3 cells were treated with RUT 500, 750, 1000, 1500 µM for 24 and 48 hours. The viability rates decreased with increasing RUT concentration depending on dose and time p0.01. Annexin V protein decreased, and apoptotic cells increased depending on the increasing RUT concentration p0.01. There was a decrease in the number of migrating cells, especially at 750 and 1000 µM RUT concentrations. There was a significant increase 14.22-fold in E-cadherin mRNA level after 1500 µM RUT treatment p0.01. On the other hand, the mRNA expression level of Snail, Twist and Vimentin, decreased at higher RUT concentrations p0.01. Conclusion: The existence of RUTs potential to inhibit epithelial-mesenchymal transition and promote apoptosis has been demonstrated. It is also recommended to investigate the effects of RUT on EMT in vivo.

 Objective: The difficulties experienced in the treatment of prostate cancer and the excess of side effects due to chemotherapy have brought the search for alternative treatment strategies. In recent studies, it is known that Rutin RUT has an anti-cancer effect on cancer cells. Our study aimed to determine the effects of RUT on epithelial-mesenchymal transition EMT in prostate cancer cells, for the first time. Methods: The anticancer activity of RUT in prostate cancer cells PC-3 was determined by WST-1, Annexin V ELISA, DAPI and Acridine Orange staining, and the anti-cancer and anti-metastatic properties of RUT were evaluated with the Scratch Assay test. The mRNA expression level of Bax, Bcl-2, Snail, Twist, Vimentin and E-cadherin genes was determined by RT-PCR. Results: PC-3 cells were treated with RUT 500, 750, 1000, 1500 µM for 24 and 48 hours. The viability rates decreased with increasing RUT concentration depending on dose and time p0.01. Annexin V protein decreased, and apoptotic cells increased depending on the increasing RUT concentration p0.01. There was a decrease in the number of migrating cells, especially at 750 and 1000 µM RUT concentrations. There was a significant increase 14.22-fold in E-cadherin mRNA level after 1500 µM RUT treatment p0.01. On the other hand, the mRNA expression level of Snail, Twist and Vimentin, decreased at higher RUT concentrations p0.01. Conclusion: The existence of RUTs potential to inhibit epithelial-mesenchymal transition and promote apoptosis has been demonstrated. It is also recommended to investigate the effects of RUT on EMT in vivo.

 Objective: The difficulties experienced in the treatment of prostate cancer and the excess of side effects due to chemotherapy have brought the search for alternative treatment strategies. In recent studies, it is known that Rutin RUT has an anti-cancer effect on cancer cells. Our study aimed to determine the effects of RUT on epithelial-mesenchymal transition EMT in prostate cancer cells, for the first time. Methods: The anticancer activity of RUT in prostate cancer cells PC-3 was determined by WST-1, Annexin V ELISA, DAPI and Acridine Orange staining, and the anti-cancer and anti-metastatic properties of RUT were evaluated with the Scratch Assay test. The mRNA expression level of Bax, Bcl-2, Snail, Twist, Vimentin and E-cadherin genes was determined by RT-PCR. Results: PC-3 cells were treated with RUT 500, 750, 1000, 1500 µM for 24 and 48 hours. The viability rates decreased with increasing RUT concentration depending on dose and time p0.01. Annexin V protein decreased, and apoptotic cells increased depending on the increasing RUT concentration p0.01. There was a decrease in the number of migrating cells, especially at 750 and 1000 µM RUT concentrations. There was a significant increase 14.22-fold in E-cadherin mRNA level after 1500 µM RUT treatment p0.01. On the other hand, the mRNA expression level of Snail, Twist and Vimentin, decreased at higher RUT concentrations p0.01. Conclusion: The existence of RUTs potential to inhibit epithelial-mesenchymal transition and promote apoptosis has been demonstrated. It is also recommended to investigate the effects of RUT on EMT in vivo.

 Amaç: Prostat kanseri tedavisinde yaşanan zorluklar, kemoterapi nedeniyle görülen yan etkilerin fazlalığı alternatif tedavi stratejileri arayışını gündeme getirmiştir. Son yıllarda yapılan araştırmalarda Rutin RUTin kanser hücreleri üzerinde anti-kanser etki gösterdiği bilinmektedir. Bu nedenle çalışmamızda RUTun prostat kanseri hücrelerinde epitelyal mezenkimal geçiş EMT üzerindeki etkilerinin ilk defa belirlenmesi amaçlanmıştır. Yöntem: Prostat kanseri hücrelerinde PC-3 RUTun antikanser aktivitesi WST-1, Annexin V ELISA, DAPI boyama ve Akridin Oranj AO boyama ile belirlenerek, RUTun anti-kanser ve anti-metastatik özellikleri Scratch Çizik Assay testi ile değerlendirildi. Bax, Bcl-2, Snail, Twist, Vimentin ve E-kaderin genlerinin mRNA ifade düzeyi RT-PCR analizi ile belirlendi. Bulgular: PC-3 hücrelerine, farklı konstrasyonlarda 500, 750, 1000, 1500 µM ve farklı zamanlarda 24 ve 48 saat RUT uygulandı. Canlılık oranlarının doza ve zamana bağlı olarak artan RUT konsantrasyonuna bağlı olarak azaldığı gözlendi p0,01. Ayrıca artan RUT konsantrasyonuna bağlı olarak Annexin V proteinin azaldığı ve apoptotik hücrelerin arttığı belirlendi p0,01. Özellikle 750 ve 1000 µM RUT konsantrasyonlarında göç eden hücre sayısında azalma olduğu ortaya kondu. RT-PCR analizi sonucu elde edilen verilerde 1500 µM RUT muamelesi sonrası E-Kaderin mRNA seviyesinde 14,22-kat olarak belirgin bir anlamlı artış olduğu belirlendi p0,01. Diğer yandan EMT sürecinde yer aldığı bilinen Snail, Twist ve Vimentin mRNA ifade düzeyinin artan RUT konsantrasyonuna bağlı olarak azaldığı ortaya konuldu p0,01. Sonuç: RUTun epitelden mezenkimal geçişi engelleme ve apoptozu teşvik edici potansiyelinin varlığı ortaya konulmuştur. Ayrıca RUTun EMT üzerine olan etkilerinin daha ileri moleküler analizler ve in vivo olarak araştırılması önerilmektedir.

Article Details

DETERMINATION OF THE EFFECT OF RUTIN ON EPITHHELIAL AND MESENCHIMAL TRANSITION IN PROSTATE CANCER CELLS

Authors:

Esra Bal | Asuman Deveci Özkan | Zeynep Betts

Publisher:

Kocaeli University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer)

Journal:

Acta Medica Nicomedia Vol.6 Issue 1, pp.131-136

Document Type:

Article

Publication Date:

2/2023

Document Language:

English

Document ID (Org No.):

2001284604

pISSN:

2717-8994

Prostate cancer

rutin

epithelial mesenchymal transition

apoptosis

Prostat kanseri

rutin

epitelyal mezenkimal geçiş

apoptoz

Issues

Article Details

Authors: Esra Bal | Asuman Deveci Özkan | Zeynep Betts

Publisher: Kocaeli University

Main Topics: Biyokimya ve Hücre Biyolojisi (Diğer)

Journal: Acta Medica Nicomedia Vol.6 Issue 1, pp.131-136

Document Type: Article

Publication Date: 2/2023

Document Language: English

Document ID (Org No.): 2001284604

pISSN: 2717-8994

Issues

Authors:

Esra Bal | Asuman Deveci Özkan | Zeynep Betts

Publisher:

Kocaeli University

Main Topics:

Biyokimya ve Hücre Biyolojisi (Diğer)

Journal:

Acta Medica Nicomedia Vol.6 Issue 1, pp.131-136

Document Type:

Article

Publication Date:

2/2023

Document Language:

English

Document ID (Org No.):

2001284604

pISSN:

2717-8994