First Record of Helvella capucina in Türkiye: Morphological and Molecular Characterization

Şuheda Aldemir Terman; Mustafa Emre Akçay; Ayten Dizkırıcı

Article Details

First Record of Helvella capucina in Türkiye: Morphological and Molecular Characterization

Authors:

Şuheda Aldemir Terman | Mustafa Emre Akçay | Ayten Dizkırıcı

Publisher:

Selcuk University

Main Topics:

Bitki ve Mantar Sistematiği ve Taksonomi

Journal:

Mantar Dergisi Vol.15 Issue Özel Sayı, pp.66-72

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001329522

pISSN:

2147-6845

Keywords:

Abstract:

 In the current study, Helvella capucina collected from Hakkari province in Türkiye is described as a new record using morphological and molecular characters. Ascocarp, hymenium, stipe, ascospores, ascus and paraphyses were used as diagnostic morphological characters. Images of microscopic characters, microphotographs of the ascospores, and colour pictures of the ascus in their natural habitats are provided. The species, which is placed in section Elasticae, was characterised by its short-stipitate apothecium and pure white when fresh, drying yellowish stipe. Molecular analysis was also carried out to characterize the new record reliably by using DNA sequences of the heat shock protein 90 HSP90, RNA polymerase 2 RPB2 and translation elongation factor 1-alpha TEF1-α. Additionally, the analyses of the three regions were combined to create a concatenated tree. The multigene phylogenetic position and evolutionary relationships of H. capucina with other species of Helvella in Elasticae were inferred using Bayesian Inferences BI of the concatenated dataset. Both morphological and molecular data separated Helvella capucina from its close relatives. Detailed descriptions, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the concatenated phylogenetic tree were provided.

 In the current study, Helvella capucina collected from Hakkari province in Türkiye is described as a new record using morphological and molecular characters. Ascocarp, hymenium, stipe, ascospores, ascus and paraphyses were used as diagnostic morphological characters. Images of microscopic characters, microphotographs of the ascospores, and colour pictures of the ascus in their natural habitats are provided. The species, which is placed in section Elasticae, was characterised by its short-stipitate apothecium and pure white when fresh, drying yellowish stipe. Molecular analysis was also carried out to characterize the new record reliably by using DNA sequences of the heat shock protein 90 HSP90, RNA polymerase 2 RPB2 and translation elongation factor 1-alpha TEF1-α. Additionally, the analyses of the three regions were combined to create a concatenated tree. The multigene phylogenetic position and evolutionary relationships of H. capucina with other species of Helvella in Elasticae were inferred using Bayesian Inferences BI of the concatenated dataset. Both morphological and molecular data separated Helvella capucina from its close relatives. Detailed descriptions, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the concatenated phylogenetic tree were provided.

 In the current study, Helvella capucina collected from Hakkari province in Türkiye is described as a new record using morphological and molecular characters. Ascocarp, hymenium, stipe, ascospores, ascus and paraphyses were used as diagnostic morphological characters. Images of microscopic characters, microphotographs of the ascospores, and colour pictures of the ascus in their natural habitats are provided. The species, which is placed in section Elasticae, was characterised by its short-stipitate apothecium and pure white when fresh, drying yellowish stipe. Molecular analysis was also carried out to characterize the new record reliably by using DNA sequences of the heat shock protein 90 HSP90, RNA polymerase 2 RPB2 and translation elongation factor 1-alpha TEF1-α. Additionally, the analyses of the three regions were combined to create a concatenated tree. The multigene phylogenetic position and evolutionary relationships of H. capucina with other species of Helvella in Elasticae were inferred using Bayesian Inferences BI of the concatenated dataset. Both morphological and molecular data separated Helvella capucina from its close relatives. Detailed descriptions, colour images of fresh and dried ascomata, along with photographs of microscopic characters and the concatenated phylogenetic tree were provided.

 Bu çalışmada, Türkiyenin Hakkari ilinden toplanan Helvella capucina türü, morfolojik ve moleküler özellikler kullanılarak yeni bir kayıt olarak tanımlanmaktadır. Belirleyici morfolojik karakterler arasında askokarp, himenyum, sap, askosporlar, askus ve parafiz yer almaktadır. Mikroskobik karakterlerin görselleri, askosporların mikrofotografik görüntüleri ve askusların doğal ortamlarındaki renkli resimleri sağlanmıştır. Elasticae seksiyonunda yer alan bu tür, kısa saplı apotezyum yapısı ve taze halde saf beyaz, kurudukça sarımsı renge dönüşen sap yapısıyla karakterizedir. Yeni kayıt türün güvenilir bir şekilde tanımlanması için ısı şok proteini 90 HSP90, RNA polimeraz 2 RPB2 ve translasyon uzatma faktörü 1-alfa TEF1-α DNA dizileri kullanılarak moleküler analizler de gerçekleştirilmiştir. Üç bölgenin analizleri birleştirilerek bir kombine ağaç oluşturulmuştur. H. capucina türünün Elasticae seksiyonunda yer alan diğer Helvella türleri ile çok genli filogenetik pozisyonu ve evrimsel ilişkileri, birleştirilmiş veri setinin Bayes Çıkarım Yöntemi BI ile çıkarım yoluyla belirlenmiştir. Hem morfolojik hem de moleküler veriler, Helvella capucina türünü yakın akrabalarından ayırmıştır. Detaylı tanım, taze ve kurumuş askokarp yapısının renkli görüntüleri, mikroskobik karakterlerin fotoğrafları ve birleştirilmiş filogenetik ağaç sunulmuştur.

Article Details

First Record of Helvella capucina in Türkiye: Morphological and Molecular Characterization

Authors:

Şuheda Aldemir Terman | Mustafa Emre Akçay | Ayten Dizkırıcı

Publisher:

Selcuk University

Main Topics:

Bitki ve Mantar Sistematiği ve Taksonomi

Journal:

Mantar Dergisi Vol.15 Issue Özel Sayı, pp.66-72

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001329522

pISSN:

2147-6845

Helvella

HSP90

new record

RPB2

TEF1-αHelvella

HSP90

RPB2

TEF1-α

yeni kayıt

Issues

Article Details

Authors: Şuheda Aldemir Terman | Mustafa Emre Akçay | Ayten Dizkırıcı

Publisher: Selcuk University

Main Topics: Bitki ve Mantar Sistematiği ve Taksonomi

Journal: Mantar Dergisi Vol.15 Issue Özel Sayı, pp.66-72

Document Type: Article

Publication Date: 12/2024

Document Language: English

Document ID (Org No.): 2001329522

pISSN: 2147-6845

Issues

Authors:

Şuheda Aldemir Terman | Mustafa Emre Akçay | Ayten Dizkırıcı

Publisher:

Selcuk University

Main Topics:

Bitki ve Mantar Sistematiği ve Taksonomi

Journal:

Mantar Dergisi Vol.15 Issue Özel Sayı, pp.66-72

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001329522

pISSN:

2147-6845