Production of Monoclonal Antibodies specific for  Progesterone

Fatıma YÜCEL

Article Details

Production of Monoclonal Antibodies specific for Progesterone

Authors:

Fatıma YÜCEL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 1999 Vol.23 Issue 4, pp.393-400

Document Type:

Article

Publication Date:

3/1999

Document Language:

Turkish

Document ID (Org No.):

2000814184

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

Progesterone levels in milk and serum are indicators of pregnancy in cattle. The  progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies  specific to progesterone could be used for the immunodetection of milk and serum progesterone  levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for  progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against  progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized  BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were  characterized for use in the development of diagnostic kits based on the enzyme-linked  immunosorbent assay (ELISA) test system. ELISA tests showed that antibodies produced by 2 hybrids  from 375 wells reacted specifically with progesterone. Monoclonal antibodies did not show any cross  reaction with other steroid hormones.

 

Progesterone levels in milk and serum are indicators of pregnancy in cattle. The  progesterone level reaches a peak on the 21 st and 22 nd days of pregnancy. Monoclonal antibodies  specific to progesterone could be used for the immunodetection of milk and serum progesterone  levels. We report here the development of hybrid cells prdoducing monoclonal antibodies specific for  progesterone using hybridoma technology. Hybridoma cells secreting monoclonal antibodies against  progesterone (MAM 2H11, MAM 6G6) were developed by fusion of the spleen cells of an immunized  BALB/c mouse with FO (ATCC CRL 1646) mouse myeloma cells. These monoclonal antibodies were  characterized for use in the development of diagnostic kits based on the enzyme-linked  immunosorbent assay (ELISA) test system. ELISA tests showed that antibodies produced by 2 hybrids  from 375 wells reacted specifically with progesterone. Monoclonal antibodies did not show any cross  reaction with other steroid hormones.

Article Details

Production of Monoclonal Antibodies specific for Progesterone

Authors:

Fatıma YÜCEL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 1999 Vol.23 Issue 4, pp.393-400

Document Type:

Article

Publication Date:

3/1999

Document Language:

Turkish

Document ID (Org No.):

2000814184

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Immunodiagnosis

monoclonal antibody

progesterone

ELISA

Issues

Article Details

Production of Monoclonal Antibodies specific for Progesterone

Authors: Fatıma YÜCEL

Publisher: TUBITAK

Journal: Turkish Journal of Biology 1999 Vol.23 Issue 4, pp.393-400

Document Type: Article

Publication Date: 3/1999

Document Language: Turkish

Document ID (Org No.): 2000814184

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Immunodiagnosis

monoclonal antibody

progesterone

ELISA

Issues

Authors:

Fatıma YÜCEL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 1999 Vol.23 Issue 4, pp.393-400

Document Type:

Article

Publication Date:

3/1999

Document Language:

Turkish

Document ID (Org No.):

2000814184

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152