Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model

Zeliha Aytekin; Ayşe Toraman; Kübra Karaçam

Article Details

Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model

Authors:

Zeliha Aytekin | Ayşe Toraman | Kübra Karaçam

Publisher:

Selcuk University

Main Topics:

Dentistry

Journal:

Selcuk Dental Journal Vol.10 Issue 2, pp.371-376

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001294036

pISSN:

2148-7529

Keywords:

Abstract:

 Background: The present study aims to evaluate the effect of local vitamin C application on inflammatory response and periodontal tissue destruction in rats with experimental periodontitis. Methods: A total of 21 animals, 7 rats in each group, were used in the study: 1- Non-ligation NL, 2-experimental periodontitis EP, and 3- local vitamin C VtC groups. Experimental periodontitis was induced in EP and VtC group rats. After 11 days, the ligature was removed. 50 mL of saline solution and vitamin C were injected locally into the vestibule sulcus of the EP and VtC group rats, respectively. After the rats were sacrificed, blood and gingival tissue samples were taken. TNF-α levels were analyzed biochemically in serum and 8-OHdG and MMP-8 were analyzed immunohistochemically in gingival tissue. The mandibular specimens were histologically evaluated for bone destruction and attachment loss. Results: The numeric density of 8-OHdG and MMP-8 immunopositive cells were found to be statistically significantly lower in the Vtc group than in the EP group. Vitamin C administration significantly reduced attachment loss and bone loss in the VtC group as compared to the EP group. However, there was no significant difference in serum TNF-α level in the VtC group compared to the EP group. Conclusions: Local application of vitamin C may reduce inflammation-related bone destruction by supporting the inflammatory response with the antioxidant activity of vitamin C and contributing to the resolution of inflammation with its potential immunomodulatory effect. Vitamin C may be a therapeutic agent that can be used in the treatment of periodontitis.

 Background: The present study aims to evaluate the effect of local vitamin C application on inflammatory response and periodontal tissue destruction in rats with experimental periodontitis. Methods: A total of 21 animals, 7 rats in each group, were used in the study: 1- Non-ligation NL, 2-experimental periodontitis EP, and 3- local vitamin C VtC groups. Experimental periodontitis was induced in EP and VtC group rats. After 11 days, the ligature was removed. 50 mL of saline solution and vitamin C were injected locally into the vestibule sulcus of the EP and VtC group rats, respectively. After the rats were sacrificed, blood and gingival tissue samples were taken. TNF-α levels were analyzed biochemically in serum and 8-OHdG and MMP-8 were analyzed immunohistochemically in gingival tissue. The mandibular specimens were histologically evaluated for bone destruction and attachment loss. Results: The numeric density of 8-OHdG and MMP-8 immunopositive cells were found to be statistically significantly lower in the Vtc group than in the EP group. Vitamin C administration significantly reduced attachment loss and bone loss in the VtC group as compared to the EP group. However, there was no significant difference in serum TNF-α level in the VtC group compared to the EP group. Conclusions: Local application of vitamin C may reduce inflammation-related bone destruction by supporting the inflammatory response with the antioxidant activity of vitamin C and contributing to the resolution of inflammation with its potential immunomodulatory effect. Vitamin C may be a therapeutic agent that can be used in the treatment of periodontitis.

 Background: The present study aims to evaluate the effect of local vitamin C application on inflammatory response and periodontal tissue destruction in rats with experimental periodontitis. Methods: A total of 21 animals, 7 rats in each group, were used in the study: 1- Non-ligation NL, 2-experimental periodontitis EP, and 3- local vitamin C VtC groups. Experimental periodontitis was induced in EP and VtC group rats. After 11 days, the ligature was removed. 50 mL of saline solution and vitamin C were injected locally into the vestibule sulcus of the EP and VtC group rats, respectively. After the rats were sacrificed, blood and gingival tissue samples were taken. TNF-α levels were analyzed biochemically in serum and 8-OHdG and MMP-8 were analyzed immunohistochemically in gingival tissue. The mandibular specimens were histologically evaluated for bone destruction and attachment loss. Results: The numeric density of 8-OHdG and MMP-8 immunopositive cells were found to be statistically significantly lower in the Vtc group than in the EP group. Vitamin C administration significantly reduced attachment loss and bone loss in the VtC group as compared to the EP group. However, there was no significant difference in serum TNF-α level in the VtC group compared to the EP group. Conclusions: Local application of vitamin C may reduce inflammation-related bone destruction by supporting the inflammatory response with the antioxidant activity of vitamin C and contributing to the resolution of inflammation with its potential immunomodulatory effect. Vitamin C may be a therapeutic agent that can be used in the treatment of periodontitis.

 Amaç: Bu çalışmanın amacı deneysel periodontitisli ratlarda lokal C vitamini uygulamasının inflamatuar yanıt ve periodontal doku yıkımı üzerine olan etkisini değerlendirmektir. Gereç ve Yöntemler: Çalışmada her grupta 7 sıçan olmak üzere toplam 21 hayvan kullanıldı: 1-Ligatür bağlanmayan NL 2-deneysel periodontitis EP ve 3-lokal vitamin C VtC grupları. EP ve VtC grubu sıçanlarda deneysel periodontitis oluşturuldu. Ligatürler 11 gün sonra ligatür çıkarıldı. EP ve VtC grubu sıçanların vestibül sulkusuna sırasıyla 50 µL salin solüsyonu ve C vitamini lokal olarak enjekte edildi. Ratlar sakrifiye edildikten sonra kan ve dişeti dokusu örnekleri alındı. TNF-α seviyesi serumda biyokimyasal olarak, 8-OHdG ve MMP-8 düzeyleri ise dişeti dokusunda immünohistokimyasal olarak analiz edildi. Mandibula örnekleri kemik yıkımı ve ataşman kaybı açısından histolojik olarak analiz edildi. Bulgular: 8-OHdG ve MMP-8 immünopozitif hücre yoğunluğunun Vtc grubunda EP grubuna göre istatistiksel olarak anlamlı derecede daha düşük olduğu bulundu. C vitamini uygulaması, EP grubuna kıyasla VtC grubunda ataşman kaybını ve kemik kaybını önemli ölçüde azalttı. Ancak EP grubuna göre VtC grubunda serum TNF-α düzeyinde anlamlı bir fark yoktu. Sonuç: Lokal C vitamini uygulaması, C vitamininin antioksidan aktivitesi ile inflamatuar yanıtı destekleyerek ve potansiyel immünomodülatör etkisi ile inflamasyonun çözülmesine katkıda bulunarak inflamasyona bağlı kemik yıkımını azaltabilir. C vitamini periodontitis tedavisinde kullanılabilecek terapötik bir ajan olabilir.

Article Details

Investigation of Effects of Local Vitamin C Application on Inflammatory Response and Periodontal Tissue Destruction in Rat Periodontitis Model

Authors:

Zeliha Aytekin | Ayşe Toraman | Kübra Karaçam

Publisher:

Selcuk University

Main Topics:

Dentistry

Journal:

Selcuk Dental Journal Vol.10 Issue 2, pp.371-376

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001294036

pISSN:

2148-7529

antioxidants

Experimental periodontitis

oxidative stress

vitamin Cantioksidanlar

Deneysel periodontitis

oksidatif stres

Vitamin C

Issues

Article Details

Authors: Zeliha Aytekin | Ayşe Toraman | Kübra Karaçam

Publisher: Selcuk University

Main Topics: Dentistry

Journal: Selcuk Dental Journal Vol.10 Issue 2, pp.371-376

Document Type: Article

Publication Date: 8/2023

Document Language: English

Document ID (Org No.): 2001294036

pISSN: 2148-7529

Issues

Authors:

Zeliha Aytekin | Ayşe Toraman | Kübra Karaçam

Publisher:

Selcuk University

Main Topics:

Dentistry

Journal:

Selcuk Dental Journal Vol.10 Issue 2, pp.371-376

Document Type:

Article

Publication Date:

8/2023

Document Language:

English

Document ID (Org No.):

2001294036

pISSN:

2148-7529