Walnut Seed Coat (Juglans regia L.), a Plant Effective in Human Health: Antioxidant Activity and in Rats Nephroprotective Effect

Esra Palabıyık; Handan Uğuz; Hakan Aşkın; Seda Aşkın; Hülya Akıncıoğlu

Article Details

Walnut Seed Coat (Juglans regia L.), a Plant Effective in Human Health: Antioxidant Activity and in Rats Nephroprotective Effect

Authors:

Esra Palabıyık | Handan Uğuz | Hakan Aşkın | Seda Aşkın | Hülya Akıncıoğlu

Publisher:

Ataturk University

Main Topics:

Biyosistem

Journal:

Research in Agricultural Sciences Vol.55 Issue 2, pp.89-104

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001317981

pISSN:

2979-9686

Keywords:

Abstract:

 In the study, the seed coat WSC of Posof ArdahanTürkiye walnuts was extracted to determine their phytochemical components and antioxidant capacities. The effects of bioactive components in the ethanol extract of WSC E-WSC on acetylcholinesterase AChE and butyrylcholinesterase BChE inhibitors were investigated. Additionally, antioxidant enzyme activity parameters were measured in the kidney tissues of Triton WR-1339-induced hyperlipidemic rats. Bioactive compounds in WSC were identified by GC-MS system. The antioxidant properties of WSC were measured using Fe3, Cu2 and Fe3-2,4,6-tripyridyl-s-triazine TPTZ reducing agent, 1,1-diphenyl-2-picrylhydrazyl DPPH and 2,2- azino-bis3-ethylbenzothiazoline-6-sulfonic acid ABTS scavenging activities. In this analysis, using 30 male Wistar rats 300  30 g randomly divided into five groups were treated as follows; K1: Healthy control group, K2: E-WSC 150 mg o.d., K3: E-WSC 300 mg o.d., K4: Hyperlipidemic group i.p., K5: Hyperlipidemic group i.p.  E-WSC 300 mg o.d. Superoxide dismutase SOD, catalase CAT and malondialdehyde MDA analyzes were performed in kidney tissues. Based on these results, it was clearly determined that E-WSC has significant antioxidant activity due to its bioactive components, has an inhibitory effect on AChE and BChE enzymes, and has a protective effect against oxidative stress by improving hyperlipidemia-related kidney damage.

 In the study, the seed coat WSC of Posof ArdahanTürkiye walnuts was extracted to determine their phytochemical components and antioxidant capacities. The effects of bioactive components in the ethanol extract of WSC E-WSC on acetylcholinesterase AChE and butyrylcholinesterase BChE inhibitors were investigated. Additionally, antioxidant enzyme activity parameters were measured in the kidney tissues of Triton WR-1339-induced hyperlipidemic rats. Bioactive compounds in WSC were identified by GC-MS system. The antioxidant properties of WSC were measured using Fe3, Cu2 and Fe3-2,4,6-tripyridyl-s-triazine TPTZ reducing agent, 1,1-diphenyl-2-picrylhydrazyl DPPH and 2,2- azino-bis3-ethylbenzothiazoline-6-sulfonic acid ABTS scavenging activities. In this analysis, using 30 male Wistar rats 300  30 g randomly divided into five groups were treated as follows; K1: Healthy control group, K2: E-WSC 150 mg o.d., K3: E-WSC 300 mg o.d., K4: Hyperlipidemic group i.p., K5: Hyperlipidemic group i.p.  E-WSC 300 mg o.d. Superoxide dismutase SOD, catalase CAT and malondialdehyde MDA analyzes were performed in kidney tissues. Based on these results, it was clearly determined that E-WSC has significant antioxidant activity due to its bioactive components, has an inhibitory effect on AChE and BChE enzymes, and has a protective effect against oxidative stress by improving hyperlipidemia-related kidney damage.

 In the study, the seed coat WSC of Posof ArdahanTürkiye walnuts was extracted to determine their phytochemical components and antioxidant capacities. The effects of bioactive components in the ethanol extract of WSC E-WSC on acetylcholinesterase AChE and butyrylcholinesterase BChE inhibitors were investigated. Additionally, antioxidant enzyme activity parameters were measured in the kidney tissues of Triton WR-1339-induced hyperlipidemic rats. Bioactive compounds in WSC were identified by GC-MS system. The antioxidant properties of WSC were measured using Fe3, Cu2 and Fe3-2,4,6-tripyridyl-s-triazine TPTZ reducing agent, 1,1-diphenyl-2-picrylhydrazyl DPPH and 2,2- azino-bis3-ethylbenzothiazoline-6-sulfonic acid ABTS scavenging activities. In this analysis, using 30 male Wistar rats 300  30 g randomly divided into five groups were treated as follows; K1: Healthy control group, K2: E-WSC 150 mg o.d., K3: E-WSC 300 mg o.d., K4: Hyperlipidemic group i.p., K5: Hyperlipidemic group i.p.  E-WSC 300 mg o.d. Superoxide dismutase SOD, catalase CAT and malondialdehyde MDA analyzes were performed in kidney tissues. Based on these results, it was clearly determined that E-WSC has significant antioxidant activity due to its bioactive components, has an inhibitory effect on AChE and BChE enzymes, and has a protective effect against oxidative stress by improving hyperlipidemia-related kidney damage.

 Çalışmada, Posof ArdahanTürkiye cevizlerinin tohum kabuğu WSC, fitokimyasal bileşenlerinin ve antioksidan kapasitelerinin belirlenmesi için ekstrakte edilmiştir. WSCnin E-WSC etanol ekstraktındaki biyoaktif bileşenlerin asetilkolinesteraz AChE ve butirilkolinesteraz BChE inhibitörleri üzerindeki etkileri araştırılmıştır. Ayrıca Triton WR-1339 ile indüklenen hiperlipidemik sıçanların böbrek dokularında antioksidan enzim aktivite parametreleri ölçülmüştür. WSCdeki biyoaktif bileşikler GC-MS sistemi ile tanımlanmıştır. WSCnin antioksidan özellikleri, Fe3, Cu2 ve Fe3-2,4,6-tripiridil-s-triazin TPTZ indirgeyici ajan, 1,1-difenil-2-pikrilhidrazil DPPH ve 2,2- azino-bis 3-etilbenzotiyazolin-6-sülfonik asit ABTS temizleme aktiviteleri kullanılarak ölçülmüştür. 30 adet erkek Wistar sıçanı 300  30 g kullanılarak yapılan bu analizde, rastgele beş gruba ayrılan sıçanlara aşağıdaki gibi uygulama yapılmıştır; K1: Sağlıklı kontrol grubu, K2: E-WSC 150 mg o.d., K3: E-WSC 300 mg o.d., K4: Hiperlipidemik grup i.p., K5: Hiperlipidemik grup i.p.  E-WSC 300 mg o.d. Böbrek dokularında Süperoksit dismutaz SOD, katalaz CAT ve malondialdehit MDA analizleri yapılmıştır. Bu sonuçlara göre E-WSCnin biyoaktif bileşenlerine bağlı olarak önemli antioksidan aktiviteye sahip olduğu, AChE ve BChE enzimleri üzerinde inhibitör etkiye sahip olduğu ve hiperlipidemiye bağlı böbrek hasarını iyileştirerek oksidatif strese karşı koruyucu etkiye sahip olduğu açıkça belirlenmiştir.

Article Details

Walnut Seed Coat (Juglans regia L.), a Plant Effective in Human Health: Antioxidant Activity and in Rats Nephroprotective Effect

Authors:

Esra Palabıyık | Handan Uğuz | Hakan Aşkın | Seda Aşkın | Hülya Akıncıoğlu

Publisher:

Ataturk University

Main Topics:

Biyosistem

Journal:

Research in Agricultural Sciences Vol.55 Issue 2, pp.89-104

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001317981

pISSN:

2979-9686

Antioxidant activity

kidney

oxidative stress

Triton WR-1339

walnut seed coatAntioksidan aktivite

böbrek

oksidatif stress

Triton WR-1339

ceviz tohumu kabuğu

Issues

Article Details

Authors: Esra Palabıyık | Handan Uğuz | Hakan Aşkın | Seda Aşkın | Hülya Akıncıoğlu

Publisher: Ataturk University

Main Topics: Biyosistem

Journal: Research in Agricultural Sciences Vol.55 Issue 2, pp.89-104

Document Type: Article

Publication Date: 5/2024

Document Language: English

Document ID (Org No.): 2001317981

pISSN: 2979-9686

Issues

Authors:

Esra Palabıyık | Handan Uğuz | Hakan Aşkın | Seda Aşkın | Hülya Akıncıoğlu

Publisher:

Ataturk University

Main Topics:

Biyosistem

Journal:

Research in Agricultural Sciences Vol.55 Issue 2, pp.89-104

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001317981

pISSN:

2979-9686