Biodegradation potential of petroleum hydrocarbons by bacteria and mixed bacterial consortium isolated from contaminated sites

Ajeet PRAKASH; Sandeep BISHT; Jagvijay SINGH; Priyanku TEOTIA; Ritu KELA; Vivek KUMAR

Article Details

Biodegradation potential of petroleum hydrocarbons by bacteria and mixed bacterial consortium isolated from contaminated sites

Authors:

Ajeet PRAKASH | Sandeep BISHT | Jagvijay SINGH | Priyanku TEOTIA | Ritu KELA | Vivek KUMAR

Publisher:

TUBITAK

Journal:

Turkish Journal of Engineering and Environmental Sciences 2014 Vol.38 Issue 1, pp.41-50

Document Type:

Article

Publication Date:

12/2013

Document Language:

Turkish

Document ID (Org No.):

2000744532

Frequency:

Tri-annual

eISSN:

1303-6157

pISSN:

1300-0160

Abstract:

 

The aim of this study was to isolate, characterize, and evaluate the  potential of petroleum hydrocarbon (PHC)-degrading bacterial strains from  oil-contaminated soil in the Meerut region. Among 59 oil-degrading bacterial  cultures isolated from the oil-contaminated soil samples, 1 Bacillus species, 2  species of Pseudomonas, and 1 species of Micrococcus, identified on the basis of biochemical and  16s rDNA sequencing, were found to have the ability to utilize PHCs such as  benzene, diesel, toluene, anthracene, and naphthalene. These strains were  selected for further study to measure the quantitative determination of PHC  metabolization. Along with these selected strains, a mixed bacterial  consortium was formulated and used for PHC degradation. Among the individual  strains, Pseudomonas sp. APHP9 performed better than the other bacterial isolates.  Maximum biodegradation of benzene and toluene was done by the bacterial  consortium. The mean growth rate constant (K) of soil isolates also  increased with a successive increase in PHC concentration. Moreover,  Bacillus sp. APHP6, Pseudomonas sp. APHP9, Pseudomonas sp. APBP1, Micrococcus sp. APIO4, and the consortium resulted  in a 54.8%, 60.2%, 40.9%, 32.5%, and 66.2% decrease in  benzene concentration and a 61.2%, 68.4%, 53.7%, 39.3%, and  75.4% decrease in diesel concentration, respectively, after 6 days of  incubation as estimated by HPLC analysis.

 

The aim of this study was to isolate, characterize, and evaluate the  potential of petroleum hydrocarbon (PHC)-degrading bacterial strains from  oil-contaminated soil in the Meerut region. Among 59 oil-degrading bacterial  cultures isolated from the oil-contaminated soil samples, 1 Bacillus species, 2  species of Pseudomonas, and 1 species of Micrococcus, identified on the basis of biochemical and  16s rDNA sequencing, were found to have the ability to utilize PHCs such as  benzene, diesel, toluene, anthracene, and naphthalene. These strains were  selected for further study to measure the quantitative determination of PHC  metabolization. Along with these selected strains, a mixed bacterial  consortium was formulated and used for PHC degradation. Among the individual  strains, Pseudomonas sp. APHP9 performed better than the other bacterial isolates.  Maximum biodegradation of benzene and toluene was done by the bacterial  consortium. The mean growth rate constant (K) of soil isolates also  increased with a successive increase in PHC concentration. Moreover,  Bacillus sp. APHP6, Pseudomonas sp. APHP9, Pseudomonas sp. APBP1, Micrococcus sp. APIO4, and the consortium resulted  in a 54.8%, 60.2%, 40.9%, 32.5%, and 66.2% decrease in  benzene concentration and a 61.2%, 68.4%, 53.7%, 39.3%, and  75.4% decrease in diesel concentration, respectively, after 6 days of  incubation as estimated by HPLC analysis.

Article Details

Biodegradation potential of petroleum hydrocarbons by bacteria and mixed bacterial consortium isolated from contaminated sites

Authors: Ajeet PRAKASH | Sandeep BISHT | Jagvijay SINGH | Priyanku TEOTIA | Ritu KELA | Vivek KUMAR

Publisher: TUBITAK

Journal: Turkish Journal of Engineering and Environmental Sciences 2014 Vol.38 Issue 1, pp.41-50

Document Type: Article

Publication Date: 12/2013

Document Language: Turkish

Document ID (Org No.): 2000744532

Frequency: Tri-annual

eISSN: 1303-6157

pISSN: 1300-0160

Issues

Authors:

Ajeet PRAKASH | Sandeep BISHT | Jagvijay SINGH | Priyanku TEOTIA | Ritu KELA | Vivek KUMAR

Publisher:

TUBITAK

Journal:

Turkish Journal of Engineering and Environmental Sciences 2014 Vol.38 Issue 1, pp.41-50

Document Type:

Article

Publication Date:

12/2013

Document Language:

Turkish

Document ID (Org No.):

2000744532

Frequency:

Tri-annual

eISSN:

1303-6157

pISSN:

1300-0160