Involvement of AMP-activated protein kinase in the protective effectof melatonin against renal ischemia reperfusion injury

KAOUTHER HADJ AYED TKA; ASMA MAHFOUDH BOUSSAID; KAOUTHER KESSABI; RYM KAMMOUN; IMED MESSAOUDI; SONIA GHOUL MAZGAR; JOAN ROSELLO CATAFAU; HASSEN BEN ABDENNEBI

Article Details

Involvement of AMP-activated protein kinase in the protective effectof melatonin against renal ischemia reperfusion injury

Authors:

KAOUTHER HADJ AYED TKA | ASMA MAHFOUDH BOUSSAID | KAOUTHER KESSABI | RYM KAMMOUN | IMED MESSAOUDI | SONIA GHOUL MAZGAR | JOAN ROSELLO CATAFAU | HASSEN BEN ABDENNEBI

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.837-844

Document Type:

Article

Publication Date:

7/2016

Document Language:

Turkish

Document ID (Org No.):

2000811621

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Abstract:

 

We investigated the implication of AMP-activated protein kinase (AMPK) in melatonin-induced protection in a renal ischemia/reperfusion (I/R) model. Animals were divided into four groups: rats of the sham group were not subjected to renal I/R. Rats in the I/R group received the vehicle 30 min before renal ischemia for 1 h followed by reperfusion for 6 h. Rats in the Mel+I/R group received melatonin (10 mg/kg) 30 min before the onset of I/R. Rats in the Mel+I/R+araA group received araA (AMPK inhibitor, 100 μg/kg per min for 10 min) just before melatonin administration. The results showed that melatonin treatment induced a significant improvement in renal function, morphology, and antioxidant status, as well as Akt pathway activation. It also decreased cytolysis and endoplasmic reticulum stress. Inhibition of AMPK by araA administration completely abolished the effects of melatonin in regard to the above parameters except for Akt and some of its downstream target molecules. These results demonstrate that the effects of melatonin on renal I/R are, for the most part, linked to AMPK activation, with the exception of the Akt pathway, which seems to be independent of AMPK.

Article Details

Involvement of AMP-activated protein kinase in the protective effectof melatonin against renal ischemia reperfusion injury

Authors: KAOUTHER HADJ AYED TKA | ASMA MAHFOUDH BOUSSAID | KAOUTHER KESSABI | RYM KAMMOUN | IMED MESSAOUDI | SONIA GHOUL MAZGAR | JOAN ROSELLO CATAFAU | HASSEN BEN ABDENNEBI

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.837-844

Document Type: Article

Publication Date: 7/2016

Document Language: Turkish

Document ID (Org No.): 2000811621

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Issues

Authors:

KAOUTHER HADJ AYED TKA | ASMA MAHFOUDH BOUSSAID | KAOUTHER KESSABI | RYM KAMMOUN | IMED MESSAOUDI | SONIA GHOUL MAZGAR | JOAN ROSELLO CATAFAU | HASSEN BEN ABDENNEBI

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.837-844

Document Type:

Article

Publication Date:

7/2016

Document Language:

Turkish

Document ID (Org No.):

2000811621

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152