Evaluation of antibiotic susceptibility in enterococci isolated from blood culture samples

Özlem Koca; Halil Er; Yeşim Çekin

Article Details

Evaluation of antibiotic susceptibility in enterococci isolated from blood culture samples

Authors:

Özlem Koca | Halil Er | Yeşim Çekin

Publisher:

MediHealth Academy Yayıncılık

Main Topics:

Tıbbi Bakteriyoloji

Journal:

Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.385-388

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001302567

pISSN:

2717-7505

Keywords:

Abstract:

 Aims: Increased vancomycin resistance in enterococci is an important cause of life-threatening bloodstream infections in hospitalized patients. The aim of this study is to determine the antibiotic susceptibility rates of Enterococcus strains isolated from blood cultures in hospitalized patients in Antalya Training and Research Hospital. Methods: The antibiotic resistance rates of Enterococcus strains isolated from blood cultures of patients hospitalized in the service and intensive care units ICU between 1 January 2018 and 30 December 2022 were examined retrospectively. Blood samples were studied with the BacTALERT 3D culture system Biomerieux, France. Bacterial identification was performed using conventional methods, Matrix Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometer MALDI-TOF MS and VITEK 2 Biomerieux, France systems. Antimicrobial susceptibility tests were performed with VITEK 2 Biomerieux, France systems. Ampicillin, vancomycin, teicoplanin, high-level gentamicin resistance HLGR and linezolid susceptibility of isolated strains were evaluated according to the European Committee on Antimicrobial Susceptibility Testing EUCAST criteria. Vancomycin minimal inhibitory concentration MIC values of vancomycin resistant strains were studied by microdilution gradient strip test Bioanalyse. Results: A total of 623 strains of enterococci were isolated from blood culture samples. Of the enterococci, 305 48.9 were identified as Enterococcus faecalis, 281 45.6 Enterococcus faecium, 12 1.9 Enterococcus avium, 11 1.8 Enterococcus gallinarum, 7 1.2 Enterococcus casseliflavus, 2 0.4 Enterococcus durans and 1 Enterococcus hirae 0.2. Ampicillin and HLGR resistance rates of isolated E. faecalis strains were 11 3.6 and 72 23.6, respectively, and all strains were found to be susceptible to vancomycin, teicoplanin and linezolid. The ampicillin, vancomycin, teicoplanin and HLGR resistance rates of E. faecium strains were determined as 229 81.5, 36 12.8, 30 10.7 and 142 50.5, respectively, and all strains were found to be susceptible to linezolid. Conclusion: In infections caused by enterococci, identification and determination of antibiotic susceptibility rates according to culture antibiogram results would be the right approach. Knowing the current susceptibility rates of enterococci isolated from blood culture samples in our hospital will contribute for clinicians planning of empirical treatment.

 Aims: Increased vancomycin resistance in enterococci is an important cause of life-threatening bloodstream infections in hospitalized patients. The aim of this study is to determine the antibiotic susceptibility rates of Enterococcus strains isolated from blood cultures in hospitalized patients in Antalya Training and Research Hospital. Methods: The antibiotic resistance rates of Enterococcus strains isolated from blood cultures of patients hospitalized in the service and intensive care units ICU between 1 January 2018 and 30 December 2022 were examined retrospectively. Blood samples were studied with the BacTALERT 3D culture system Biomerieux, France. Bacterial identification was performed using conventional methods, Matrix Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometer MALDI-TOF MS and VITEK 2 Biomerieux, France systems. Antimicrobial susceptibility tests were performed with VITEK 2 Biomerieux, France systems. Ampicillin, vancomycin, teicoplanin, high-level gentamicin resistance HLGR and linezolid susceptibility of isolated strains were evaluated according to the European Committee on Antimicrobial Susceptibility Testing EUCAST criteria. Vancomycin minimal inhibitory concentration MIC values of vancomycin resistant strains were studied by microdilution gradient strip test Bioanalyse. Results: A total of 623 strains of enterococci were isolated from blood culture samples. Of the enterococci, 305 48.9 were identified as Enterococcus faecalis, 281 45.6 Enterococcus faecium, 12 1.9 Enterococcus avium, 11 1.8 Enterococcus gallinarum, 7 1.2 Enterococcus casseliflavus, 2 0.4 Enterococcus durans and 1 Enterococcus hirae 0.2. Ampicillin and HLGR resistance rates of isolated E. faecalis strains were 11 3.6 and 72 23.6, respectively, and all strains were found to be susceptible to vancomycin, teicoplanin and linezolid. The ampicillin, vancomycin, teicoplanin and HLGR resistance rates of E. faecium strains were determined as 229 81.5, 36 12.8, 30 10.7 and 142 50.5, respectively, and all strains were found to be susceptible to linezolid. Conclusion: In infections caused by enterococci, identification and determination of antibiotic susceptibility rates according to culture antibiogram results would be the right approach. Knowing the current susceptibility rates of enterococci isolated from blood culture samples in our hospital will contribute for clinicians planning of empirical treatment.

 Aims: Increased vancomycin resistance in enterococci is an important cause of life-threatening bloodstream infections in hospitalized patients. The aim of this study is to determine the antibiotic susceptibility rates of Enterococcus strains isolated from blood cultures in hospitalized patients in Antalya Training and Research Hospital. Methods: The antibiotic resistance rates of Enterococcus strains isolated from blood cultures of patients hospitalized in the service and intensive care units ICU between 1 January 2018 and 30 December 2022 were examined retrospectively. Blood samples were studied with the BacTALERT 3D culture system Biomerieux, France. Bacterial identification was performed using conventional methods, Matrix Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometer MALDI-TOF MS and VITEK 2 Biomerieux, France systems. Antimicrobial susceptibility tests were performed with VITEK 2 Biomerieux, France systems. Ampicillin, vancomycin, teicoplanin, high-level gentamicin resistance HLGR and linezolid susceptibility of isolated strains were evaluated according to the European Committee on Antimicrobial Susceptibility Testing EUCAST criteria. Vancomycin minimal inhibitory concentration MIC values of vancomycin resistant strains were studied by microdilution gradient strip test Bioanalyse. Results: A total of 623 strains of enterococci were isolated from blood culture samples. Of the enterococci, 305 48.9 were identified as Enterococcus faecalis, 281 45.6 Enterococcus faecium, 12 1.9 Enterococcus avium, 11 1.8 Enterococcus gallinarum, 7 1.2 Enterococcus casseliflavus, 2 0.4 Enterococcus durans and 1 Enterococcus hirae 0.2. Ampicillin and HLGR resistance rates of isolated E. faecalis strains were 11 3.6 and 72 23.6, respectively, and all strains were found to be susceptible to vancomycin, teicoplanin and linezolid. The ampicillin, vancomycin, teicoplanin and HLGR resistance rates of E. faecium strains were determined as 229 81.5, 36 12.8, 30 10.7 and 142 50.5, respectively, and all strains were found to be susceptible to linezolid. Conclusion: In infections caused by enterococci, identification and determination of antibiotic susceptibility rates according to culture antibiogram results would be the right approach. Knowing the current susceptibility rates of enterococci isolated from blood culture samples in our hospital will contribute for clinicians planning of empirical treatment.

 Amaç: Enterokoklarda artan antibiyotik direnci hastanede yatan hastalarda hayatı tehdit eden kan dolaşımı infeksiyonlarının önemli bir nedenidir. Bu çalışmanın amacı, Antalya Eğitim ve Araştırma Hastanesinde yatan hastalarda, kan kültürlerinden izole edilen Enterokok suşlarında antibiyotik duyarlılık oranlarının belirlenmesidir. Yöntem: Hastanemiz servis ve yoğun bakım ünitelerinde YBÜ yatan hastaların bilgi işlem sistemi LIS üzerinden, 1 Ocak 2018- 30 Aralık 2022 tarihlerinde, kan kültürü örneklerinden izole edilen Enterococcus suşlarının antibiyotik direnç oranları retrospektif olarak incelenmiştir. Kan örnekleri, BacTALERT 3D kültürü sisteminde Biomerieux, France inkübe edilmiştir. Bakteri identifikasyonu konvansiyonel yöntemler, Matriks Destekli Lazer Dezorpsiyon-İyonizasyon Uçuş Süresi Kütle Spektrometresi MALDI-TOF MS ve VITEK 2 Biomerieux, France sistemleri kullanılarak yapılmıştır. Antimikrobiyal duyarlılık testleri VITEK 2 Biomerieux, France sistemleri ile çalışılmıştır. İzole edilen suşların ampisilin, vankomisin, teikoplanin, yüksek düzey gentamisin direnci YDGR ve linezolid duyarlılıkları European Committee on Antimicrobial Susceptibility Testing EUCAST kriterlerine göre değerlendirilmiştir. Vankomisine dirençli suşların, mikrodilüsyon gradient strip test Bioanalyse ile vankomisin minimal inhibitör konsantrasyon MİK değerleri çalışılmıştır. Bulgular: Kan kültürü örneklerinden toplam 623 enterokok suşu izole edilmiştir. Enterokokların 305i 48.9 Enterococcus faecalis, 281i 45.6 Enterococcus faecium, 12si 1.9 Enterococcus avium, 11i 1.8 Enterococcus gallinarum, 7si 1.2 Enterococcus casseliflavus, 2si 0.4 Enterococcus durans ve 1i 0.2 Enterococcus hirae olarak tanımlanmıştır. İzole edilen E. faecalis suşlarının ampisilin ve YDGR direnç oranları sırayla 11 3.6 ve 72 23.6 olarak saptanmış ve bütün suşlar vankomisin, teikoplanin ve linezolide duyarlı bulunmuştur. E. faecium suşlarının ampisilin, vankomisin, teikoplanin ve YDGR direnç oranları sırayla 229 81.5, 36 12.8, 30 10.7 ve 142 50.5 olarak saptanmıştır ve bütün suşlar linezolide duyarlı olarak bulunmuştur. Sonuç: Enterokoklarla oluşan enfeksiyonlarda, identifikasyon ve antibiyotik duyarlılık oranlarının kültür antibiyogram sonuçlarına göre belirlenmesi doğru yaklaşım olacaktır. Kan kültürü örneklerinden izole edilen enterokokların, hastanemize ait güncel duyarlılık oranlarının bilinmesi, klinisyenlerin ampirik tedavi planlanmasına katkı sağlayacaktır.

Article Details

Evaluation of antibiotic susceptibility in enterococci isolated from blood culture samples

Authors:

Özlem Koca | Halil Er | Yeşim Çekin

Publisher:

MediHealth Academy Yayıncılık

Main Topics:

Tıbbi Bakteriyoloji

Journal:

Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.385-388

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001302567

pISSN:

2717-7505

Enterococci

blood culture

antibiotic susceptibilitiesEnterokoklar

kan kültürü

antibiyotik duyarlılıkları

Issues

Article Details

Evaluation of antibiotic susceptibility in enterococci isolated from blood culture samples

Authors: Özlem Koca | Halil Er | Yeşim Çekin

Publisher: MediHealth Academy Yayıncılık

Main Topics: Tıbbi Bakteriyoloji

Journal: Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.385-388

Document Type: Article

Publication Date: 10/2023

Document Language: English

Document ID (Org No.): 2001302567

pISSN: 2717-7505

Enterococci

blood culture

antibiotic susceptibilitiesEnterokoklar

kan kültürü

antibiyotik duyarlılıkları

Issues

Authors:

Özlem Koca | Halil Er | Yeşim Çekin

Publisher:

MediHealth Academy Yayıncılık

Main Topics:

Tıbbi Bakteriyoloji

Journal:

Journal of Medicine and Palliative Care Vol.4 Issue 5, pp.385-388

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001302567

pISSN:

2717-7505