Novel Metal Accumulator and Protease Secretor Microbes from East Calcutta Wetland

Sanhita Chowdhury; Madhusmita Mishra; V.K. Adarsh; Anindita Mukherjee; Ashoke Ranjan Thakur; Shaon Ray Chaudhuri

doi:10.3844/ajbbsp.2008.255.264

Article Details

Novel Metal Accumulator and Protease Secretor Microbes from East Calcutta Wetland

Authors:

Sanhita Chowdhury | Madhusmita Mishra | V.K. Adarsh | Anindita Mukherjee | Ashoke Ranjan Thakur | Shaon Ray Chaudhuri

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.4 Issue 3, pp.255-264

Document Type:

Article

Publication Date:

9/2008

Document Language:

English

Document ID (Org No.):

2001396972

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2008.255.264

Keywords:

Abstract:

 Nine bacterial isolates were screened from different sites of East Calcutta Wetland, an ecosystem located at the eastern fringes of Calcutta. Other than being a biodiversity rich area, the important feature of this system is that it is a natural sewage treatment plant for the city of Calcutta. In addition to daily sewage including solid and soluble wastes, a considerable load of toxic metals are released into the water bodies from industries, tanneries, agriculture, household as well as health sectors. Screening out microbes from such an environment was done keeping in mind their multi functional application. These bacterial isolates were found to produce extracellular protease which is known to have vast applications in the commercial market of enzymes. The key area of this study is exploring the role of these isolates in heavy metal remediation. These isolates were found to tolerate heavy metals like Ag, Al, Cu, Cr, Co, Ni and Pb. Energy Dispersive X Ray Fluorescence (EDXRF) analysis and Transmission Electron Microscopy of the metal treated cells confirm the intracellular accumulation. Moreover, a preliminary demonstration of the effect of metal induced stress on cell surface features was determined by Scanning Electron Microscopy.

Article Details

Novel Metal Accumulator and Protease Secretor Microbes from East Calcutta Wetland

Authors:

Sanhita Chowdhury | Madhusmita Mishra | V.K. Adarsh | Anindita Mukherjee | Ashoke Ranjan Thakur | Shaon Ray Chaudhuri

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.4 Issue 3, pp.255-264

Document Type:

Article

Publication Date:

9/2008

Document Language:

English

Document ID (Org No.):

2001396972

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2008.255.264

East Calcutta Wetland

EDXRF

TEM

SEM

nanoparticle

protease secreting bacteria

Issues

Article Details

Novel Metal Accumulator and Protease Secretor Microbes from East Calcutta Wetland

Authors: Sanhita Chowdhury | Madhusmita Mishra | V.K. Adarsh | Anindita Mukherjee | Ashoke Ranjan Thakur | Shaon Ray Chaudhuri

Publisher: Science Publications

Journal: American Journal of Biochemistry and Biotechnology Vol.4 Issue 3, pp.255-264

Document Type: Article

Publication Date: 9/2008

Document Language: English

Document ID (Org No.): 2001396972

pISSN: 1558-6332

DOI: 10.3844/ajbbsp.2008.255.264

East Calcutta Wetland

EDXRF

TEM

SEM

nanoparticle

protease secreting bacteria

Issues

Authors:

Sanhita Chowdhury | Madhusmita Mishra | V.K. Adarsh | Anindita Mukherjee | Ashoke Ranjan Thakur | Shaon Ray Chaudhuri

Publisher:

Science Publications

Journal:

American Journal of Biochemistry and Biotechnology Vol.4 Issue 3, pp.255-264

Document Type:

Article

Publication Date:

9/2008

Document Language:

English

Document ID (Org No.):

2001396972

pISSN:

1558-6332

DOI:

10.3844/ajbbsp.2008.255.264