A Simple and Rapid HPLC-UV Method for the Determination of Umckalin , as an Herbal Marker , in the Cough Syrup of Pelargonium Extract

Hudaib , M.; Aldahasi , W. B.; Khdair , A.

Article Details

A Simple and Rapid HPLC-UV Method for the Determination of Umckalin , as an Herbal Marker , in the Cough Syrup of Pelargonium Extract

Authors:

Hudaib , M. | Aldahasi , W. B. | Khdair , A.

Publisher:

University of Jordan : Deanship of Academic Research

Main Topics:

Pharmacology and therapeutics

Journal:

Jordan Journal of Pharmaceutical Sciences 2010 vol.3 Issue 2, pp.109-116

Document Type:

Article

Publication Date:

7/2010

Document Language:

English

Document ID (Org No.):

20825

Frequency:

Bi-Annual

pISSN:

19957157

Descriptors:

Keywords:

Abstract:

 A rapid and simple high performance liquid chromatography (HPLC) method with UV detection for thequantitation of umckalin, as an herbal marker, in Pelargonium extract cough syrup has been developed andvalidated. Chromatographic separation was achieved on a reverse phase Phenomenex®-C18 column (5 μm, 25 cm× 0.5 mm i.d.) using a mixture of acetonitrile and phosphoric acid (pH 2.5), in 25:75 (v/v) ratio, as a mobilephase at a flow rate of 1 mL/min under ambient conditions and with UV detection at 310 nm. The method,applied for umckalin quantitation, showed good linearity over the concentration range of 0.334 –1.667 μg/mL,with a correlation coefficient (r2) of 0.9996. The limit of detection (LOD) and limit of quantitation (LOQ) ofumckalin were found to be 0.0344 and 0.1031 μg/mL, respectively. In addition, the developed HPLC methodshowed acceptable values of repeatability and intermediate precision and indicated high levels of methodaccuracy. Simplicity and validity of the method make it highly reliable and especially suitable for routinequality control analysis.

Article Details

A Simple and Rapid HPLC-UV Method for the Determination of Umckalin , as an Herbal Marker , in the Cough Syrup of Pelargonium Extract

Authors:

Hudaib , M. | Aldahasi , W. B. | Khdair , A.

Publisher:

University of Jordan : Deanship of Academic Research

Main Topics:

Pharmacology and therapeutics

Journal:

Jordan Journal of Pharmaceutical Sciences 2010 vol.3 Issue 2, pp.109-116

Document Type:

Article

Publication Date:

7/2010

Document Language:

English

Document ID (Org No.):

20825

Frequency:

Bi-Annual

pISSN:

19957157

Descriptors:

PHARMACEUTICALS

MEDICINAL PLANTS

CHROMATOGRAPHY

PELARGONIUM

ULTRAVIOLET RADIATION

COUGH

RESPIRATORY SYSTEM AGENTS

JORDAN

Umckalin

Pelargonium sidoides

HPLC analysis

Cough syrup

Herbal product

Herbal marker.

Issues

Article Details

Authors: Hudaib , M. | Aldahasi , W. B. | Khdair , A.

Publisher: University of Jordan : Deanship of Academic Research

Main Topics: Pharmacology and therapeutics

Journal: Jordan Journal of Pharmaceutical Sciences 2010 vol.3 Issue 2, pp.109-116

Document Type: Article

Publication Date: 7/2010

Document Language: English

Document ID (Org No.): 20825

Frequency: Bi-Annual

pISSN: 19957157

Descriptors:

Issues

Authors:

Hudaib , M. | Aldahasi , W. B. | Khdair , A.

Publisher:

University of Jordan : Deanship of Academic Research

Main Topics:

Pharmacology and therapeutics

Journal:

Jordan Journal of Pharmaceutical Sciences 2010 vol.3 Issue 2, pp.109-116

Document Type:

Article

Publication Date:

7/2010

Document Language:

English

Document ID (Org No.):

20825

Frequency:

Bi-Annual

pISSN:

19957157