The Effect of Hazelnut Oil on Intra-Abdominal Adhesion: An Experimental Study in a Rat Model

Çağrı Akalın; Özlem Özdemir; Nergis Ekmen; Mürüvvet Akçay Çelik

Article Details

The Effect of Hazelnut Oil on Intra-Abdominal Adhesion: An Experimental Study in a Rat Model

Authors:

Çağrı Akalın | Özlem Özdemir | Nergis Ekmen | Mürüvvet Akçay Çelik

Publisher:

Yozgat Bozok University

Main Topics:

Gastroenteroloji Cerrahisi | Genel Cerrahi

Journal:

Bozok Tıp Dergisi Vol.15 Issue 3, pp.331-336

Document Type:

Article

Publication Date:

9/2025

Document Language:

English

Document ID (Org No.):

2001358233

pISSN:

2146-4006

Keywords:

Abstract:

 Aim: Intra-abdominal adhesions are a common complication of abdominopelvic surgery, contributing to chronic pain, infertility, and intestinal obstruction. Oxidative stress and inflammation play central roles in their pathogenesis, underscoring the need for effective preventive agents with antioxidant and anti-inflammatory properties. This study aimed to evaluate the potential anti-adhesive effects of topically applied hazelnut oil compared to Seprafilm in a standardised rat model of peritoneal injury. Material and Methods: Twenty-eight Sprague-Dawley rats were randomly assigned to four groups: Sham hazelnut oil only, Adhesion injury without treatment, Adhesion  Hazelnut Oil, and Adhesion  Seprafilm. Adhesions were induced via standardised cecal abrasion. Hazelnut oil 5 mL or Seprafilm 30  20 mm was applied intraperitoneally. After 14 days, macroscopic adhesions were scored using the Evans classification, and histopathological evaluation was conducted using the Zühlke score. Results: Significant differences were observed in macroscopic Evans Score and histopathological Zühlke Score adhesion scores among groups p  0.001. The Sham group exhibited significantly lower scores than the Adhesion group p  0.001. The Hazelnut Oil and Seprafilm groups showed lower median scores compared to the Adhesion group, but these differences were not statistically significant p  0.05. The Hazelnut oil showed a trend toward reduced histopathological fibrosis, similar to Seprafilm, suggesting a potential protective effect. Conclusion: These findings suggest that hazelnut oil may have a role in reducing intra-abdominal adhesions, though the effect was not statistically significant in this small sample. Its natural composition and potential biochemical benefits warrant further investigation as a cost-effective anti-adhesive agent.

 Aim: Intra-abdominal adhesions are a common complication of abdominopelvic surgery, contributing to chronic pain, infertility, and intestinal obstruction. Oxidative stress and inflammation play central roles in their pathogenesis, underscoring the need for effective preventive agents with antioxidant and anti-inflammatory properties. This study aimed to evaluate the potential anti-adhesive effects of topically applied hazelnut oil compared to Seprafilm in a standardised rat model of peritoneal injury. Material and Methods: Twenty-eight Sprague-Dawley rats were randomly assigned to four groups: Sham hazelnut oil only, Adhesion injury without treatment, Adhesion  Hazelnut Oil, and Adhesion  Seprafilm. Adhesions were induced via standardised cecal abrasion. Hazelnut oil 5 mL or Seprafilm 30  20 mm was applied intraperitoneally. After 14 days, macroscopic adhesions were scored using the Evans classification, and histopathological evaluation was conducted using the Zühlke score. Results: Significant differences were observed in macroscopic Evans Score and histopathological Zühlke Score adhesion scores among groups p  0.001. The Sham group exhibited significantly lower scores than the Adhesion group p  0.001. The Hazelnut Oil and Seprafilm groups showed lower median scores compared to the Adhesion group, but these differences were not statistically significant p  0.05. The Hazelnut oil showed a trend toward reduced histopathological fibrosis, similar to Seprafilm, suggesting a potential protective effect. Conclusion: These findings suggest that hazelnut oil may have a role in reducing intra-abdominal adhesions, though the effect was not statistically significant in this small sample. Its natural composition and potential biochemical benefits warrant further investigation as a cost-effective anti-adhesive agent.

 Aim: Intra-abdominal adhesions are a common complication of abdominopelvic surgery, contributing to chronic pain, infertility, and intestinal obstruction. Oxidative stress and inflammation play central roles in their pathogenesis, underscoring the need for effective preventive agents with antioxidant and anti-inflammatory properties. This study aimed to evaluate the potential anti-adhesive effects of topically applied hazelnut oil compared to Seprafilm in a standardised rat model of peritoneal injury. Material and Methods: Twenty-eight Sprague-Dawley rats were randomly assigned to four groups: Sham hazelnut oil only, Adhesion injury without treatment, Adhesion  Hazelnut Oil, and Adhesion  Seprafilm. Adhesions were induced via standardised cecal abrasion. Hazelnut oil 5 mL or Seprafilm 30  20 mm was applied intraperitoneally. After 14 days, macroscopic adhesions were scored using the Evans classification, and histopathological evaluation was conducted using the Zühlke score. Results: Significant differences were observed in macroscopic Evans Score and histopathological Zühlke Score adhesion scores among groups p  0.001. The Sham group exhibited significantly lower scores than the Adhesion group p  0.001. The Hazelnut Oil and Seprafilm groups showed lower median scores compared to the Adhesion group, but these differences were not statistically significant p  0.05. The Hazelnut oil showed a trend toward reduced histopathological fibrosis, similar to Seprafilm, suggesting a potential protective effect. Conclusion: These findings suggest that hazelnut oil may have a role in reducing intra-abdominal adhesions, though the effect was not statistically significant in this small sample. Its natural composition and potential biochemical benefits warrant further investigation as a cost-effective anti-adhesive agent.

 Amaç: İntraabdominal adezyonlar, abdominopelvik cerrahilerin sık görülen komplikasyonlarından biridir ve kronik ağrı, infertilite ve intestinal obstrüksiyon gibi ciddi klinik sonuçlara yol açabilir. Oksidatif stres ve inflamasyon bu sürecin patogenezinde merkezi bir rol oynamaktadır. Bu nedenle, antioksidan ve anti-inflamatuar özelliklere sahip etkili önleyici ajanlara ihtiyaç duyulmaktadır. Bu çalışmada, peritoneal yaralanma modeli oluşturulan sıçanlarda, topikal olarak uygulanan fındık yağının anti-adezif potansiyeli Seprafilm ile karşılaştırmalı olarak değerlendirilmiştir. Gereç ve Yöntem: Yirmi sekiz Sprague-Dawley cinsi sıçan rastgele dört gruba ayrılmıştır: Sham yalnızca fındık yağı uygulanan, Adezyon yaralanma ancak tedavi uygulanmayan, Adezyon  Fındık Yağı ve Adezyon  Seprafilm grupları. Adezyonlar, standartize edilmiş çekal abrazyon yöntemi ile indüklenmiştir. Fındık yağı 5 mL ya da Seprafilm 30  20 mm intraperitoneal olarak uygulanmıştır. On dört gün sonra makroskopik adezyonlar Evans sınıflandırması ile değerlendirilmiş, histopatolojik inceleme ise Zühlke skoru kullanılarak yapılmıştır. Bulgular: Makroskopik Evans skoru ve histopatolojik Zühlke skoru adezyon derecelerinde gruplar arasında anlamlı fark saptanmıştır p  0.001. Sham grubunun skorları, Adezyon grubuna kıyasla anlamlı olarak daha düşük bulunmuştur p  0.001. Fındık Yağı ve Seprafilm gruplarının medyan skorları Adezyon grubuna göre daha düşük olmasına rağmen, bu farklar istatistiksel olarak anlamlı bulunmamıştır p  0.05. Fındık yağı, Seprafilm ile benzer şekilde histopatolojik fibrozisi azaltma eğilimi göstermiştir. Sonuç: Bu bulgular, fındık yağının intraabdominal adezyonların azaltılmasında potansiyel bir role sahip olabileceğini göstermektedir. Her ne kadar bu çalışma istatistiksel anlamlılık ortaya koymasa da, fındık yağının doğal içeriği ve biyokimyasal faydaları göz önüne alındığında, maliyet etkin bir anti-adezif ajan olarak daha kapsamlı araştırmalara konu edilmesi gerektiği düşünülmektedir.

Article Details

The Effect of Hazelnut Oil on Intra-Abdominal Adhesion: An Experimental Study in a Rat Model

Authors:

Çağrı Akalın | Özlem Özdemir | Nergis Ekmen | Mürüvvet Akçay Çelik

Publisher:

Yozgat Bozok University

Main Topics:

Gastroenteroloji Cerrahisi | Genel Cerrahi

Journal:

Bozok Tıp Dergisi Vol.15 Issue 3, pp.331-336

Document Type:

Article

Publication Date:

9/2025

Document Language:

English

Document ID (Org No.):

2001358233

pISSN:

2146-4006

Intra-abdominal adhesions

Hazelnut oil

Seprafilm

Antioxidants

Peritoneal injury

Rat modelİntraabdominal adezyonlar

Fındık yağı

Seprafilm

Antioksidanlar

Peritoneal yaralanma

Rat modeli

Issues

Article Details

Authors: Çağrı Akalın | Özlem Özdemir | Nergis Ekmen | Mürüvvet Akçay Çelik

Publisher: Yozgat Bozok University

Main Topics: Gastroenteroloji Cerrahisi | Genel Cerrahi

Journal: Bozok Tıp Dergisi Vol.15 Issue 3, pp.331-336

Document Type: Article

Publication Date: 9/2025

Document Language: English

Document ID (Org No.): 2001358233

pISSN: 2146-4006

Issues

Authors:

Çağrı Akalın | Özlem Özdemir | Nergis Ekmen | Mürüvvet Akçay Çelik

Publisher:

Yozgat Bozok University

Main Topics:

Gastroenteroloji Cerrahisi | Genel Cerrahi

Journal:

Bozok Tıp Dergisi Vol.15 Issue 3, pp.331-336

Document Type:

Article

Publication Date:

9/2025

Document Language:

English

Document ID (Org No.):

2001358233

pISSN:

2146-4006