Effect of phosphodiesterase-5 inhibition on joint and muscle damage in rats with  adjuvant arthritis

Faize Elif BAHADIR; Mustafa Kutay KÖROĞLU; Meral YÜKSEL; Feriha ERCAN; Yaşar İnci ALİCAN

Article Details

Effect of phosphodiesterase-5 inhibition on joint and muscle damage in rats with adjuvant arthritis

Authors:

Faize Elif BAHADIR | Mustafa Kutay KÖROĞLU | Meral YÜKSEL | Feriha ERCAN | Yaşar İnci ALİCAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 3, pp.635-643

Document Type:

Article

Publication Date:

5/2018

Document Language:

Turkish

Document ID (Org No.):

2000750751

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Abstract:

 

Background/aim: This study was designed to examine the effect of tadalafil, a phosphodiesterase (PDE)5 inhibitor, on the severity of  joint and muscle damage in rats with adjuvant-induced arthritis (AA).  Materials and methods: AA was induced by intradermal inoculation into right hind paw of male Sprague Dawley rats (300-450g) with complete Freund's adjuvant (CFA; 0.1 mL). AA rats were treated with either tadalafil (10 mg/kg; per oral) alone or along  with the soluble guanylyl cyclase inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ; 10 mg/kg; intraperitoneally). After  decapitation on day 16, trunk blood was collected for total oxidant status (TOS) and total antioxidant capacity (TAC) assays. The left  metatarsophalangeal joint and gastrocnemius muscle were excised for microscopic examination. Muscle samples were also evaluated in  terms of malondialdehyde (MDA), glutathione, and chemiluminescence (CL) levels.  Results: In tadalafil-treated AA rats, metatarsophalangeal joints revealed regular morphology of the cartilage with slight destruction  and less inflammatory cell infiltration and vascularization in comparison to the controls (microscopic score: 1.17 ± 0.31 vs. 4.17 ± 0.79;  P < 0.01). AA rats presented increased gastrocnemius muscle MDA, glutathione, and CL levels compared to the controls (P < 0.01, for  MDA; P < 0.05, for glutathione; P < 0.05 for CL). Tadalafil attenuated the increase in CL levels (P < 0.01, for luminol and P < 0.001, for  lucigenin). Serum TOS showed significant reductions by tadalafil.  Conclusion: The long-acting PDE5 inhibitor tadalafil provides partial protection in a rat model of CFA-induced arthritis possibly via  suppression of oxidant generation.

Article Details

Effect of phosphodiesterase-5 inhibition on joint and muscle damage in rats with adjuvant arthritis

Authors: Faize Elif BAHADIR | Mustafa Kutay KÖROĞLU | Meral YÜKSEL | Feriha ERCAN | Yaşar İnci ALİCAN

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2018 Vol.48 Issue 3, pp.635-643

Document Type: Article

Publication Date: 5/2018

Document Language: Turkish

Document ID (Org No.): 2000750751

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Issues

Authors:

Faize Elif BAHADIR | Mustafa Kutay KÖROĞLU | Meral YÜKSEL | Feriha ERCAN | Yaşar İnci ALİCAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2018 Vol.48 Issue 3, pp.635-643

Document Type:

Article

Publication Date:

5/2018

Document Language:

Turkish

Document ID (Org No.):

2000750751

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144