Examination of Cognitive Functions and Hippocampal Synaptophysin Levels in an Experimental Schizophrenia Model

Erkan Ermiş; Cansu Koç; Hilmiye Şule Mergen; İbrahim Makinecioğlu; Ayşe Pınar Vural; Mehmet Cansev; Gökhan Göktalay; Şafak Eray Çamlı

Article Details

Examination of Cognitive Functions and Hippocampal Synaptophysin Levels in an Experimental Schizophrenia Model

Authors:

Erkan Ermiş | Cansu Koç | Hilmiye Şule Mergen | İbrahim Makinecioğlu | Ayşe Pınar Vural | Mehmet Cansev | Gökhan Göktalay | Şafak Eray Çamlı

Publisher:

Bursa Uludağ University

Main Topics:

Sinirbilim (Diğer)

Journal:

Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.49 Issue 3, pp.367-373

Document Type:

Article

Publication Date:

12/2023

Document Language:

Turkish

Document ID (Org No.):

2001305805

pISSN:

1300-414X

Keywords:

Abstract:

 Schizophrenia is a chronic brain disease and is clinically characterized by positive, negative and cognitive symptoms. Cognitive symptoms can be observed starting from the prodromal period of the disease. The aim of this study is to investigate the cognitive functions of rats and hippocampal levels of the presynaptic protein synaptophysin in an experimental schizophrenia model created by prepulse-mediated inhibition PPI of the startle reflex. In the study, 30 male Wistar type rats were subjected to basal PPI measurements and were ranked from low to high according to these values. After the first 10 rats were divided into the low and the last 10 rats into the high inhibition group, they were subjected to the Morris Water Maze MWM test for 5 days. At the end of the test, the rats were sacrificed, hippocampus regions were excised, and the presynaptic synaptophysin protein was analyzed by Western Blot method. According to the results, while there was no difference between the learning levels of both groups, the frequency of memory functions frequency to cross the platform area p 0.05 and time spent in the platform area p 0.05 parameters were found to be significantly lower in rats in the low PPI group. Synaptophysin levels were similarly found to be significantly p0.01 lower in rats in the low PPI group. The results of our study show that some cognitive functions and presynaptic protein synaptophysin levels are significantly lower in rats with low PPI values compared to those with high PPI values. These results also supported the importance of the PPI test, which has been used as a reliable method in schizophrenia research for a long time, by emphasizing its comparable outcomes in human and animal studies of schizophrenia.

 Schizophrenia is a chronic brain disease and is clinically characterized by positive, negative and cognitive symptoms. Cognitive symptoms can be observed starting from the prodromal period of the disease. The aim of this study is to investigate the cognitive functions of rats and hippocampal levels of the presynaptic protein synaptophysin in an experimental schizophrenia model created by prepulse-mediated inhibition PPI of the startle reflex. In the study, 30 male Wistar type rats were subjected to basal PPI measurements and were ranked from low to high according to these values. After the first 10 rats were divided into the low and the last 10 rats into the high inhibition group, they were subjected to the Morris Water Maze MWM test for 5 days. At the end of the test, the rats were sacrificed, hippocampus regions were excised, and the presynaptic synaptophysin protein was analyzed by Western Blot method. According to the results, while there was no difference between the learning levels of both groups, the frequency of memory functions frequency to cross the platform area p 0.05 and time spent in the platform area p 0.05 parameters were found to be significantly lower in rats in the low PPI group. Synaptophysin levels were similarly found to be significantly p0.01 lower in rats in the low PPI group. The results of our study show that some cognitive functions and presynaptic protein synaptophysin levels are significantly lower in rats with low PPI values compared to those with high PPI values. These results also supported the importance of the PPI test, which has been used as a reliable method in schizophrenia research for a long time, by emphasizing its comparable outcomes in human and animal studies of schizophrenia.

 Schizophrenia is a chronic brain disease and is clinically characterized by positive, negative and cognitive symptoms. Cognitive symptoms can be observed starting from the prodromal period of the disease. The aim of this study is to investigate the cognitive functions of rats and hippocampal levels of the presynaptic protein synaptophysin in an experimental schizophrenia model created by prepulse-mediated inhibition PPI of the startle reflex. In the study, 30 male Wistar type rats were subjected to basal PPI measurements and were ranked from low to high according to these values. After the first 10 rats were divided into the low and the last 10 rats into the high inhibition group, they were subjected to the Morris Water Maze MWM test for 5 days. At the end of the test, the rats were sacrificed, hippocampus regions were excised, and the presynaptic synaptophysin protein was analyzed by Western Blot method. According to the results, while there was no difference between the learning levels of both groups, the frequency of memory functions frequency to cross the platform area p 0.05 and time spent in the platform area p 0.05 parameters were found to be significantly lower in rats in the low PPI group. Synaptophysin levels were similarly found to be significantly p0.01 lower in rats in the low PPI group. The results of our study show that some cognitive functions and presynaptic protein synaptophysin levels are significantly lower in rats with low PPI values compared to those with high PPI values. These results also supported the importance of the PPI test, which has been used as a reliable method in schizophrenia research for a long time, by emphasizing its comparable outcomes in human and animal studies of schizophrenia.

 Şizofreni pozitif, negatif ve bilişsel belirtiler ile seyreden kronik bir beyin hastalığıdır. Bilişsel belirtiler hastalığın prodromal döneminden itibaren gözlenebilmektedir. Bu çalışmanın amacı irkilme refleksininin ön uyaran aracılı inhibisyonu ÖUAİ ile oluşturulan deneysel şizofreni modelinde sıçanların bilişsel fonksiyonlarını ve hipokampal presinaptik proteinlerden sinaptofizin düzeylerini araştırmaktır. Çalışmada 30 adet erkek Wistar türü sıçanlar bazal ÖUAİ ölçümüne tabi tutulmuş ve bu değerlere göre düşükten yükseğe sıralanmıştır. İlk 10 sıçan düşük ve son 10 sıçan yüksek inhibisyonlu grup olarak ayrıldıktan sonra 5 gün boyunca Morris Su Tankı MST testine tabi tutulmuştur. Testin bitiminde sıçanlar sakrifiye edilerek hipokampus bölgeleri eksize edilmiş ve hipokampal presinaptik proteinlerden sinaptofizin Western Blot yöntemiyle analiz edilmiştir. Sonuçlara göre her iki grubun öğrenme düzeyleri arasında fark bulunmaz iken ve hafıza fonksiyonlarının platform alanından geçme sıklığı p0,05 ve platform alanında geçirilen süre parametreleri p0,05 düşük ÖUAİ gruptaki sıçanlarda anlamlı olarak daha düşük bulunmuştur. Sinaptofizin düzeyleri de benzer şekilde düşük ÖUAİ grubundaki sıçanlarda anlamlı olarak p0,01 düşük tespit edilmiştir. Çalışmamızın sonuçları yüksek ÖUAİ değerine sahip sıçanlarla kıyaslandığında ÖUAİ değerleri düşük olan sıçanların bazı bilişsel fonksiyonlarının ve hipokampal presinaptik proteinlerden sinaptofizin düzeylerinin anlamlı olarak daha düşük olduğunu göstermektedir. Bu sonuçlar aynı zamanda uzun zamandır şizofreni çalışmalarında güvenilir bir yöntem olarak kullanılan ÖUAİ testinin insan ve hayvan çalışmalarındaki benzer sonuçlarına vurgu yaparak şizofreni araştırmalarındaki önemini desteklemiştir.

Article Details

Examination of Cognitive Functions and Hippocampal Synaptophysin Levels in an Experimental Schizophrenia Model

Authors:

Erkan Ermiş | Cansu Koç | Hilmiye Şule Mergen | İbrahim Makinecioğlu | Ayşe Pınar Vural | Mehmet Cansev | Gökhan Göktalay | Şafak Eray Çamlı

Publisher:

Bursa Uludağ University

Main Topics:

Sinirbilim (Diğer)

Journal:

Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.49 Issue 3, pp.367-373

Document Type:

Article

Publication Date:

12/2023

Document Language:

Turkish

Document ID (Org No.):

2001305805

pISSN:

1300-414X

Schizophrenia

Prepulse Inhibition

Endophenotype

Synaptophysin

MemoryŞizofreni

Ön Uyaran Aracılı İnhibisyon

Endofenotip

Sinaptofizin

Hafıza

Issues

Article Details

Authors: Erkan Ermiş | Cansu Koç | Hilmiye Şule Mergen | İbrahim Makinecioğlu | Ayşe Pınar Vural | Mehmet Cansev | Gökhan Göktalay | Şafak Eray Çamlı

Publisher: Bursa Uludağ University

Main Topics: Sinirbilim (Diğer)

Journal: Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.49 Issue 3, pp.367-373

Document Type: Article

Publication Date: 12/2023

Document Language: Turkish

Document ID (Org No.): 2001305805

pISSN: 1300-414X

Issues

Authors:

Erkan Ermiş | Cansu Koç | Hilmiye Şule Mergen | İbrahim Makinecioğlu | Ayşe Pınar Vural | Mehmet Cansev | Gökhan Göktalay | Şafak Eray Çamlı

Publisher:

Bursa Uludağ University

Main Topics:

Sinirbilim (Diğer)

Journal:

Uludağ Üniversitesi Tıp Fakültesi Dergisi Vol.49 Issue 3, pp.367-373

Document Type:

Article

Publication Date:

12/2023

Document Language:

Turkish

Document ID (Org No.):

2001305805

pISSN:

1300-414X