Evaluating the Production of Doubled Haploid Wheat Lines Using Various Methods of Wheat and Maize Crossing to Develop Heat-Tolerant Wheat Varieties

Tayebeh BAKHSHI; Reza BOZORGIPOUR; Farajollah SHAHRIARI-AHMADI

Article Details

Evaluating the Production of Doubled Haploid Wheat Lines Using Various Methods of Wheat and Maize Crossing to Develop Heat-Tolerant Wheat Varieties

Authors:

Tayebeh BAKHSHI | Reza BOZORGIPOUR | Farajollah SHAHRIARI-AHMADI

Publisher:

Sivas Cumhuriyet Üniversitesi

Journal:

Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 2017 Vol.38 Issue 1, pp.64-78

Document Type:

Article

Publication Date:

2/2017

Document Language:

Turkish

Document ID (Org No.):

2000921065

Frequency:

Quarterly

eISSN:

2587-246X

pISSN:

2587-2680

Abstract:

 


Abstract. In this study, chromosome elimination method was used to develop doubled haploid wheat lines via crosses with maize. The plant materials used included 11, F1 wheat genotypes and maize genotype BC572. In these crosses, the maize plant was used as the male parent.Three methods of haploid production in wheat comprising conventional (A), detached-tiller culture (B) and intermediate (C) techniques were used and compared. The traits such as the number of seeds set, the number of embryos obtained and the number of haploid seedlings produced were studied. Comparisons showed that among various methods of storing wheat spikes, method (C) was better than other techniques in terms of the percentage of seed production, embryo formation and haploid seedling production. Also, in all three methods, the percentage of seed production, the percentage of embryo formation and the percentage of haploid seedling production were respectively equal to 76.84, 25.22 and 51.89. Among the wheat genotypes in all three methods, genotype DH-133 with 87.28 percent seed set and genotype DH-132 with 32.71 percent embryo formation and 65.08 percent haploid seedling production were the best genotypes. A total of 92 doubled haploid lines were produced. In the field evaluations of 86 doubled haploid lines, traits such as growing season, plant height, lodging, kernel yield and 1000 kernel weight were examined. Finally, 3 lines were selected for adaptation and stability testing under heat stress conditions.

Keywords: Wheat, Doubled haploid, Chromosome elimination, Detached-tiller culture

 

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Article Details

Evaluating the Production of Doubled Haploid Wheat Lines Using Various Methods of Wheat and Maize Crossing to Develop Heat-Tolerant Wheat Varieties

Authors: Tayebeh BAKHSHI | Reza BOZORGIPOUR | Farajollah SHAHRIARI-AHMADI

Publisher: Sivas Cumhuriyet Üniversitesi

Journal: Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 2017 Vol.38 Issue 1, pp.64-78

Document Type: Article

Publication Date: 2/2017

Document Language: Turkish

Document ID (Org No.): 2000921065

Frequency: Quarterly

eISSN: 2587-246X

pISSN: 2587-2680

Issues

Authors:

Tayebeh BAKHSHI | Reza BOZORGIPOUR | Farajollah SHAHRIARI-AHMADI

Publisher:

Sivas Cumhuriyet Üniversitesi

Journal:

Cumhuriyet Üniversitesi Fen Edebiyat Fakültesi Fen Bilimleri Dergisi 2017 Vol.38 Issue 1, pp.64-78

Document Type:

Article

Publication Date:

2/2017

Document Language:

Turkish

Document ID (Org No.):

2000921065

Frequency:

Quarterly

eISSN:

2587-246X

pISSN:

2587-2680