Investigation of the Interaction between Fat body and Ovary Development during Pupal Transformation in Silkworm, Bombyx mori

Esen POYRAZ; Ebru GÖNCÜ; Osman PARLAK

Article Details

Investigation of the Interaction between Fat body and Ovary Development during Pupal Transformation in Silkworm, Bombyx mori

Authors:

Esen POYRAZ | Ebru GÖNCÜ | Osman PARLAK

Publisher:

Istanbul University

Journal:

European Journal of Biology 2013 Vol.72 Issue 2, pp.1-13

Document Type:

Article

Publication Date:

5/2014

Document Language:

English

Document ID (Org No.):

2000424557

eISSN:

2618-6144

pISSN:

2602-2575

Abstract:

 


The insect fat body is the major biosynthetic and storage organ involved in lipid, carbohydrate, amino

acid and nitrogen metabolism and protein synthesis. It also actively participates in vitellogenesis and

ovary development, synthesizing the soluble precursor for yolk, i.e., vitellogenin in stage specific

manner. The continual substance exchange among fat body, hemolymph and ovary is controlled by

hormones, ecdysteroids and juvenile hormone, during metamorphosis of

Bombyx mori 

(L.). The aim

of the present study was to clarify the interactions between major proteins of fat body and hemolymph

 

and their effect on ovarian development under hormonal factors during pupal-adult transformation.

 

Detected fat body, hemolymph and ovary proteins are grouped as follows by using sodium dodecyl

 

sulfate polyacrylamide gel electrophoresis (SDS-PAGE): storage proteins (72 kDa and 76 kDa), 30

 

kDa proteins, ApoLp-I (230-250 kDa), egg specific protein (72 kDa and 64 kDa) and vitellogenins

 

(178 kDa). Our results suggest that changes in the well-defined and characterized protein fractions

 

quality and quantity of the fat body had a direct effect under hormonal factors on the ovary and egg

 

development during metamorphosis.

 

 

.

 

Böcek yağ dokusu, lipit, karbohidrat, aminoasit ve nitrojen metabolizmalarına katılan ve protein sentezinde rol alan en büyük biyosentetik depo organıdır. Bunun yanı sıra, vitellogenin gibi yumurta depo proteinlerinin öncülerini sentezleyerek aktif olarak vitellogenezde ve ovaryum gelişiminde rol alır. Bombyx mori’de (L.)’te metamorfoz süresince, yağ doku, hemolenf ve ovaryum arasında meydana gelen sürekli madde değişimi ekdisteroidler ve juvenile hormonun kontrolü altında gerçekleşir. Elektroforetik olarak belirlenen temel hemolenf proteinleri şu şekilde gruplandırılmıştır: depo proteinleri (72 kDa ve 76 kDa), 30K polipeptidleri, ApoLp-I (230- 250 kDa), yumurta spesifik proteini (/2 kDa ve 64 kDa) ve vitellogenin (178 kDa). Çalışmamızın sonucunda metamorfoz süresince yağ doku proteinlerinin nitelik ve niceliğindeki değişimlerin ovaryum ve yumurta gelişimi üzerinde doğrudan etkiye sahip olduğu sonucuna ulaşılmıştır.

Article Details

Investigation of the Interaction between Fat body and Ovary Development during Pupal Transformation in Silkworm, Bombyx mori

Authors: Esen POYRAZ | Ebru GÖNCÜ | Osman PARLAK

Publisher: Istanbul University

Journal: European Journal of Biology 2013 Vol.72 Issue 2, pp.1-13

Document Type: Article

Publication Date: 5/2014

Document Language: English

Document ID (Org No.): 2000424557

eISSN: 2618-6144

pISSN: 2602-2575

Issues

Authors:

Esen POYRAZ | Ebru GÖNCÜ | Osman PARLAK

Publisher:

Istanbul University

Journal:

European Journal of Biology 2013 Vol.72 Issue 2, pp.1-13

Document Type:

Article

Publication Date:

5/2014

Document Language:

English

Document ID (Org No.):

2000424557

eISSN:

2618-6144

pISSN:

2602-2575