Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model

Rana SIRMALI; Zeynep GİNİŞ; Mehmet SIRMALI; Okan SOLAK; Bengü ŞELİMAN; Yetkin AĞAÇKIRAN; Namık DELİBAŞ

Article Details

Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model

Authors:

Rana SIRMALI | Zeynep GİNİŞ | Mehmet SIRMALI | Okan SOLAK | Bengü ŞELİMAN | Yetkin AĞAÇKIRAN | Namık DELİBAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2014 Vol.44 Issue 6, pp.905-913

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000752939

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

To analyze the protective activity of vitamin C on the lungs by assessing biochemical and histopathological analysis after performing an experimental isolated lung contusion model.   Materials and methods: Fifty-four male Sprague-Dawley male rats were used. The rats were randomly separated into 4 groups. Vitamin C (200 mg/kg) was injected intraperitoneally 30 min after trauma. Blood samples were obtained for myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and malondialdehyde (MDA) levels. Blood gas analysis and bronchoalveolar lavage was performed. The lung tissue was also extracted for histopathological examination.   Results: The lung contusion enhanced MDA, SOD, CAT, and MPO and diminished GSH-Px. Vitamin C administration after the pulmonary contusion was found to diminish the level of MDA and the activities of SOD, CAT, and MPO and to enhance the level of GSH-Px (P < 0.05). Contusion-induced disrupted gas analysis and leukocyte infiltration were both resolved by the vitamin C.   Conclusion: The present results indicate that vitamin C administration attenuated the oxidative damage and morphological changes induced by pulmonary contusion in an experimental rat study.

 

To analyze the protective activity of vitamin C on the lungs by assessing biochemical and histopathological analysis after performing an experimental isolated lung contusion model.   Materials and methods: Fifty-four male Sprague-Dawley male rats were used. The rats were randomly separated into 4 groups. Vitamin C (200 mg/kg) was injected intraperitoneally 30 min after trauma. Blood samples were obtained for myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) activities and malondialdehyde (MDA) levels. Blood gas analysis and bronchoalveolar lavage was performed. The lung tissue was also extracted for histopathological examination.   Results: The lung contusion enhanced MDA, SOD, CAT, and MPO and diminished GSH-Px. Vitamin C administration after the pulmonary contusion was found to diminish the level of MDA and the activities of SOD, CAT, and MPO and to enhance the level of GSH-Px (P < 0.05). Contusion-induced disrupted gas analysis and leukocyte infiltration were both resolved by the vitamin C.   Conclusion: The present results indicate that vitamin C administration attenuated the oxidative damage and morphological changes induced by pulmonary contusion in an experimental rat study.

Article Details

Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model

Authors:

Rana SIRMALI | Zeynep GİNİŞ | Mehmet SIRMALI | Okan SOLAK | Bengü ŞELİMAN | Yetkin AĞAÇKIRAN | Namık DELİBAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2014 Vol.44 Issue 6, pp.905-913

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000752939

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Vitamin C

antioxidant

lung injury

pulmonary contusion

Issues

Article Details

Vitamin C as an antioxidant: evaluation of its role on pulmonary contusion experimental model

Authors: Rana SIRMALI | Zeynep GİNİŞ | Mehmet SIRMALI | Okan SOLAK | Bengü ŞELİMAN | Yetkin AĞAÇKIRAN | Namık DELİBAŞ

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2014 Vol.44 Issue 6, pp.905-913

Document Type: Article

Publication Date: 5/2014

Document Language: Turkish

Document ID (Org No.): 2000752939

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Vitamin C

antioxidant

lung injury

pulmonary contusion

Issues

Authors:

Rana SIRMALI | Zeynep GİNİŞ | Mehmet SIRMALI | Okan SOLAK | Bengü ŞELİMAN | Yetkin AĞAÇKIRAN | Namık DELİBAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2014 Vol.44 Issue 6, pp.905-913

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000752939

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144