Hepatitis G virus and its prevalence and genotypes in patients with hepatitis B and C in Ahvaz, southwestern Iran

Javanmard DAVOD; Makvandi MANOOCHEHR; Hajiani ESKANDAR

Article Details

Hepatitis G virus and its prevalence and genotypes in patients with hepatitis B and C in Ahvaz, southwestern Iran

Authors:

Javanmard DAVOD | Makvandi MANOOCHEHR | Hajiani ESKANDAR

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.474-478

Document Type:

Article

Publication Date:

2/2013

Document Language:

Turkish

Document ID (Org No.):

2000753757

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

The aim of this study was to determine the prevalence and genotype distribution of hepatitis G virus (HGV, or GB virus C) in patients with hepatitis B and C in Ahvaz, southeastern Iran.  Materials and methods: A total of 100 patients were selected; 50 were positive for hepatitis C virus (HCV) and 50 for hepatitis B virus (HBV). HGV status was examined among the HCV- and HBV-positive patients. An enzyme-linked immunosorbent assay (ELISA) kit was used to detect anti-E2 antibody. Nested reverse transcription polymerase chain reaction (RT-PCR) was used for RNA detection from serum samples of chronic hepatitis patients. Sequencing was done to determine genotypes  Results: Using the ELISA method, co-infection of HGV with HCV and HBV was determined at 6% and 8%, respectively. RT-PCR showed that co-infection of HGV with HCV and HBV was 22% and 10%, respectively. Based on nucleotide sequencing of PCR products, the predominant genotype of HGV among the samples was 2a.   Conclusion: Our study showed that the co-infection rate of HGV in patients with hepatitis B and C was somewhat high and was higher in HCV- than in HBV-infected patients. As our findings and other reports from Iran and neighboring countries indicate, genotype 2 of HGV may be the most common genotype of HGV in Middle Eastern countries.

 

The aim of this study was to determine the prevalence and genotype distribution of hepatitis G virus (HGV, or GB virus C) in patients with hepatitis B and C in Ahvaz, southeastern Iran.  Materials and methods: A total of 100 patients were selected; 50 were positive for hepatitis C virus (HCV) and 50 for hepatitis B virus (HBV). HGV status was examined among the HCV- and HBV-positive patients. An enzyme-linked immunosorbent assay (ELISA) kit was used to detect anti-E2 antibody. Nested reverse transcription polymerase chain reaction (RT-PCR) was used for RNA detection from serum samples of chronic hepatitis patients. Sequencing was done to determine genotypes  Results: Using the ELISA method, co-infection of HGV with HCV and HBV was determined at 6% and 8%, respectively. RT-PCR showed that co-infection of HGV with HCV and HBV was 22% and 10%, respectively. Based on nucleotide sequencing of PCR products, the predominant genotype of HGV among the samples was 2a.   Conclusion: Our study showed that the co-infection rate of HGV in patients with hepatitis B and C was somewhat high and was higher in HCV- than in HBV-infected patients. As our findings and other reports from Iran and neighboring countries indicate, genotype 2 of HGV may be the most common genotype of HGV in Middle Eastern countries.

Article Details

Hepatitis G virus and its prevalence and genotypes in patients with hepatitis B and C in Ahvaz, southwestern Iran

Authors:

Javanmard DAVOD | Makvandi MANOOCHEHR | Hajiani ESKANDAR

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.474-478

Document Type:

Article

Publication Date:

2/2013

Document Language:

Turkish

Document ID (Org No.):

2000753757

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Hepatitis G virus

hepatitis B virus

hepatitis C virus

prevalence

genotype

reverse transcription polymerase chain reaction

Issues

Article Details

Hepatitis G virus and its prevalence and genotypes in patients with hepatitis B and C in Ahvaz, southwestern Iran

Authors: Javanmard DAVOD | Makvandi MANOOCHEHR | Hajiani ESKANDAR

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.474-478

Document Type: Article

Publication Date: 2/2013

Document Language: Turkish

Document ID (Org No.): 2000753757

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Hepatitis G virus

hepatitis B virus

hepatitis C virus

prevalence

genotype

reverse transcription polymerase chain reaction

Issues

Authors:

Javanmard DAVOD | Makvandi MANOOCHEHR | Hajiani ESKANDAR

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 3, pp.474-478

Document Type:

Article

Publication Date:

2/2013

Document Language:

Turkish

Document ID (Org No.):

2000753757

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144