Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro

Serap ÇELİKLER KASIMOĞULLARI; Seyhan ORAN; Ferda ARI; Engin ULUKAYA

doi:https://doi.org/10.3906/biy-1405-23

Article Details

Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro

Authors:

Serap ÇELİKLER KASIMOĞULLARI | Seyhan ORAN | Ferda ARI | Engin ULUKAYA

Publisher:

TUBITAK

Main Topics:

Life sciences

Journal:

Turkish Journal of Biology 2014 Vol.38 Issue 6, pp.940-947

Document Type:

Article

Publication Date:

5/2014

Document Language:

English

Document ID (Org No.):

2000812098

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1405-23

Keywords:

Abstract:

 

Lichens are recently receiving great attention because they have potential anticancer activity. Therefore, in this study, genotoxic and antigrowth properties of lichen species Usnea filipendula Stirt. were tested against human breast cancer cell lines (MCF-7 and MDA-MB-231). Antigrowth effect was assayed by the MTT and ATP viability assays. Cell death modes (apoptosis/necrosis) were evaluated morphologically (fluorescence staining) and biochemically (caspase-cleaved cytokeratin 18, caspase-3 activity, and poly-(ADP-ribose) polymerase (PARP) cleavage). Genotoxic activity of U. filipendula was determined by using micronucleus, chromosomal aberration, and comet assays in human lymphocyte culture. U. filipendula inhibited growth in a dose-dependent manner and induced apoptosis by cleavage of PARP and induction of active caspase-3. It also showed genotoxic activity in doses (125 and 250 µg/mL) higher than that required for apoptosis. These results suggest that U. filipendula may induce apoptotic cell death at lower doses, while it may be genotoxic at higher doses.

 

Lichens are recently receiving great attention because they have potential anticancer activity. Therefore, in this study, genotoxic and antigrowth properties of lichen species Usnea filipendula Stirt. were tested against human breast cancer cell lines (MCF-7 and MDA-MB-231). Antigrowth effect was assayed by the MTT and ATP viability assays. Cell death modes (apoptosis/necrosis) were evaluated morphologically (fluorescence staining) and biochemically (caspase-cleaved cytokeratin 18, caspase-3 activity, and poly-(ADP-ribose) polymerase (PARP) cleavage). Genotoxic activity of U. filipendula was determined by using micronucleus, chromosomal aberration, and comet assays in human lymphocyte culture. U. filipendula inhibited growth in a dose-dependent manner and induced apoptosis by cleavage of PARP and induction of active caspase-3. It also showed genotoxic activity in doses (125 and 250 µg/mL) higher than that required for apoptosis. These results suggest that U. filipendula may induce apoptotic cell death at lower doses, while it may be genotoxic at higher doses.

Article Details

Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro

Authors:

Serap ÇELİKLER KASIMOĞULLARI | Seyhan ORAN | Ferda ARI | Engin ULUKAYA

Publisher:

TUBITAK

Main Topics:

Life sciences

Journal:

Turkish Journal of Biology 2014 Vol.38 Issue 6, pp.940-947

Document Type:

Article

Publication Date:

5/2014

Document Language:

English

Document ID (Org No.):

2000812098

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1405-23

Usnea filipendula

breast cancer

cell death

apoptosis

DNA damage

Issues

Article Details

Genotoxic, cytotoxic, and apoptotic effects of crude extract of Usnea filipendula Stirt. in vitro

Authors: Serap ÇELİKLER KASIMOĞULLARI | Seyhan ORAN | Ferda ARI | Engin ULUKAYA

Publisher: TUBITAK

Main Topics: Life sciences

Journal: Turkish Journal of Biology 2014 Vol.38 Issue 6, pp.940-947

Document Type: Article

Publication Date: 5/2014

Document Language: English

Document ID (Org No.): 2000812098

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-1405-23

Usnea filipendula

breast cancer

cell death

apoptosis

DNA damage

Issues

Authors:

Serap ÇELİKLER KASIMOĞULLARI | Seyhan ORAN | Ferda ARI | Engin ULUKAYA

Publisher:

TUBITAK

Main Topics:

Life sciences

Journal:

Turkish Journal of Biology 2014 Vol.38 Issue 6, pp.940-947

Document Type:

Article

Publication Date:

5/2014

Document Language:

English

Document ID (Org No.):

2000812098

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1405-23