Production, purification and identification of Glycine,N-(m-anisoyl)-methyl ester from Pseudomonas aeruginosa with antimicrobial and anticancer activities.

Sawsan Abd Ellatif; Nermine N. Abed; Amira Y. Mahfouz; Enayat M. Desouky; Dalia K. Abd El Hamid

Article Details

Production, purification and identification of Glycine,N-(m-anisoyl)-methyl ester from Pseudomonas aeruginosa with antimicrobial and anticancer activities.

Authors:

Sawsan Abd Ellatif | Nermine N. Abed | Amira Y. Mahfouz | Enayat M. Desouky | Dalia K. Abd El Hamid

Publisher:

Kuwait University

Journal:

Kuwait Journal of Science Vol.48 Issue 3, pp.0-0

Document Type:

Article

Publication Date:

6/2021

Document Language:

English

Document ID (Org No.):

2001943092

pISSN:

2307-4116

Keywords:

Abstract:

 The present research is a trial to extract antimicrobial and anticancer substance(s) from Pseudomonas aeruginosa. The bacterial isolate S1B20 exhibited the highest antimicrobial activity and is identified on the basis of 16S rRNA gene sequence as Pseudomonas aeruginosa MG429777. Extraction of various antimicrobial substance(s) (both proteinous and non-proteinous substances) were carried out. About applying the extract of antimicrobial substances from Pseudomonas aeruginosa MG 429777, chloroform established to be the strongest solvent for extraction of the antimicrobial substance, while 60% ammonium sulfate saturation stage was the brightest to obtain active bacteriocin fraction. The molecular weight of the refined bacteriocins estimated as 124 kDa. Quantitative results of GC-mass spectral analysis and IR spectroscopy of nonproteinaceous and proteinous antimicrobial agent delivered by Pseudomonas aeruginosa MG429777 was Oxime methoxy phenyl and Glycine, N-(m-anisoyl)-methyl ester. The research concern mainly on the application of Glycine, N-(m-anisoyl)-methyl ester that proved to have a distinct antibacterial and antitumor force against three carcinoma cell lines.

Article Details

Production, purification and identification of Glycine,N-(m-anisoyl)-methyl ester from Pseudomonas aeruginosa with antimicrobial and anticancer activities.

Authors:

Sawsan Abd Ellatif | Nermine N. Abed | Amira Y. Mahfouz | Enayat M. Desouky | Dalia K. Abd El Hamid

Publisher:

Kuwait University

Journal:

Kuwait Journal of Science Vol.48 Issue 3, pp.0-0

Document Type:

Article

Publication Date:

6/2021

Document Language:

English

Document ID (Org No.):

2001943092

pISSN:

2307-4116

Antibiotic resistance

Antimicrobial

Pseudomonas aeruginosa

Glycine

N-(m-anisoyl)-methyl ester

IR spectroscopy

16S rRNA gene

GC-mass

Issues

Article Details

Authors: Sawsan Abd Ellatif | Nermine N. Abed | Amira Y. Mahfouz | Enayat M. Desouky | Dalia K. Abd El Hamid

Publisher: Kuwait University

Journal: Kuwait Journal of Science Vol.48 Issue 3, pp.0-0

Document Type: Article

Publication Date: 6/2021

Document Language: English

Document ID (Org No.): 2001943092

pISSN: 2307-4116

Issues

Authors:

Sawsan Abd Ellatif | Nermine N. Abed | Amira Y. Mahfouz | Enayat M. Desouky | Dalia K. Abd El Hamid

Publisher:

Kuwait University

Journal:

Kuwait Journal of Science Vol.48 Issue 3, pp.0-0

Document Type:

Article

Publication Date:

6/2021

Document Language:

English

Document ID (Org No.):

2001943092

pISSN:

2307-4116