Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples

Ebrar Şeyma Özdemir; Sümeyye Koç; Demet Özçelik; Selman Aktaş; Mehmet Murat Yekrek; Esra Paydaş Hataysal; Mevlüt Sait Keleş; Fatih Gültekin; Eray Metin Güler

Article Details

Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples

Authors:

Ebrar Şeyma Özdemir | Sümeyye Koç | Demet Özçelik | Selman Aktaş | Mehmet Murat Yekrek | Esra Paydaş Hataysal | Mevlüt Sait Keleş | Fatih Gültekin | Eray Metin Güler

Publisher:

Istanbul University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

İstanbul Journal of Pharmacy Vol.54 Issue 3, pp.417-423

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001340659

pISSN:

2587-2087

Keywords:

Abstract:

 Background and Aims: In vitro and in vivo studies have shown that Zingiber officinale Z. officinale, Ginger may have an anticoagulant effect. Although there are studies on the anticoagulant effect, the results are inconclusive. Our study investigated the anticoagulant effect of Z. officinale on the whole blood sample ex vivo. Methods: The inner and shell parts of Z. officinale were extracted with methanol, ethanol, and water. 0.1 mgmL of different volumes 100, 150 and 200 μL of Z. officinale extracts were added to the blood of a healthy volunteer ex vivo. Prothrombin time PT and activated partial thromboplastin time aPTT were measured in the coagulation analyser. Measurements were performed twice, before and after the ginger treatment. International Normalised Ratio INR values were calculated using the following mathematical formula: INR  Patient PTControl PT. The IBM SPSS 25.0 software was used for statistical analyses. Results: A notable prolongation in PT, aPTT, and INR was detected after the addition of Z. officinale extract to blood samples p0.001. As the volume of Z. officinale extract added to the blood sample increased, coagulation parameters were observed to display a corresponding increase p0.001. Conclusion: Z. officinale was associated with prolonged PT, aPTT, and INR ex vivo. In vivo studies are needed to demonstrate the mechanism of the anticoagulant effect.

 Background and Aims: In vitro and in vivo studies have shown that Zingiber officinale Z. officinale, Ginger may have an anticoagulant effect. Although there are studies on the anticoagulant effect, the results are inconclusive. Our study investigated the anticoagulant effect of Z. officinale on the whole blood sample ex vivo. Methods: The inner and shell parts of Z. officinale were extracted with methanol, ethanol, and water. 0.1 mgmL of different volumes 100, 150 and 200 μL of Z. officinale extracts were added to the blood of a healthy volunteer ex vivo. Prothrombin time PT and activated partial thromboplastin time aPTT were measured in the coagulation analyser. Measurements were performed twice, before and after the ginger treatment. International Normalised Ratio INR values were calculated using the following mathematical formula: INR  Patient PTControl PT. The IBM SPSS 25.0 software was used for statistical analyses. Results: A notable prolongation in PT, aPTT, and INR was detected after the addition of Z. officinale extract to blood samples p0.001. As the volume of Z. officinale extract added to the blood sample increased, coagulation parameters were observed to display a corresponding increase p0.001. Conclusion: Z. officinale was associated with prolonged PT, aPTT, and INR ex vivo. In vivo studies are needed to demonstrate the mechanism of the anticoagulant effect.

 Background and Aims: In vitro and in vivo studies have shown that Zingiber officinale Z. officinale, Ginger may have an anticoagulant effect. Although there are studies on the anticoagulant effect, the results are inconclusive. Our study investigated the anticoagulant effect of Z. officinale on the whole blood sample ex vivo. Methods: The inner and shell parts of Z. officinale were extracted with methanol, ethanol, and water. 0.1 mgmL of different volumes 100, 150 and 200 μL of Z. officinale extracts were added to the blood of a healthy volunteer ex vivo. Prothrombin time PT and activated partial thromboplastin time aPTT were measured in the coagulation analyser. Measurements were performed twice, before and after the ginger treatment. International Normalised Ratio INR values were calculated using the following mathematical formula: INR  Patient PTControl PT. The IBM SPSS 25.0 software was used for statistical analyses. Results: A notable prolongation in PT, aPTT, and INR was detected after the addition of Z. officinale extract to blood samples p0.001. As the volume of Z. officinale extract added to the blood sample increased, coagulation parameters were observed to display a corresponding increase p0.001. Conclusion: Z. officinale was associated with prolonged PT, aPTT, and INR ex vivo. In vivo studies are needed to demonstrate the mechanism of the anticoagulant effect.

 Background and Aims: In vitro and in vivo studies have shown that Zingiber officinale Z. officinale, Ginger may have an anticoagulant effect. Although there are studies on the anticoagulant effect, the results are inconclusive. Our study investigated the anticoagulant effect of Z. officinale on the whole blood sample ex vivo. Methods: The inner and shell parts of Z. officinale were extracted with methanol, ethanol, and water. 0.1 mgmL of different volumes 100, 150 and 200 μL of Z. officinale extracts were added to the blood of a healthy volunteer ex vivo. Prothrombin time PT and activated partial thromboplastin time aPTT were measured in the coagulation analyser. Measurements were performed twice, before and after the ginger treatment. International Normalised Ratio INR values were calculated using the following mathematical formula: INR  Patient PTControl PT. The IBM SPSS 25.0 software was used for statistical analyses. Results: A notable prolongation in PT, aPTT, and INR was detected after the addition of Z. officinale extract to blood samples p0.001. As the volume of Z. officinale extract added to the blood sample increased, coagulation parameters were observed to display a corresponding increase p0.001. Conclusion: Z. officinale was associated with prolonged PT, aPTT, and INR ex vivo. In vivo studies are needed to demonstrate the mechanism of the anticoagulant effect.

Article Details

Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples

Authors:

Ebrar Şeyma Özdemir | Sümeyye Koç | Demet Özçelik | Selman Aktaş | Mehmet Murat Yekrek | Esra Paydaş Hataysal | Mevlüt Sait Keleş | Fatih Gültekin | Eray Metin Güler

Publisher:

Istanbul University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

İstanbul Journal of Pharmacy Vol.54 Issue 3, pp.417-423

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001340659

pISSN:

2587-2087

Activated partial thromboplastin time

Anticoagulant herbs

Ginger

Prothrombin time

Zingiber officinale

Issues

Article Details

Ex vivo anticoagulant effect of Zingiber officinale in whole blood samples

Authors: Ebrar Şeyma Özdemir | Sümeyye Koç | Demet Özçelik | Selman Aktaş | Mehmet Murat Yekrek | Esra Paydaş Hataysal | Mevlüt Sait Keleş | Fatih Gültekin | Eray Metin Güler

Publisher: Istanbul University

Main Topics: Pharmacology and Pharmaceutical Sciences (Other)

Journal: İstanbul Journal of Pharmacy Vol.54 Issue 3, pp.417-423

Document Type: Article

Publication Date: 12/2024

Document Language: English

Document ID (Org No.): 2001340659

pISSN: 2587-2087

Activated partial thromboplastin time

Anticoagulant herbs

Ginger

Prothrombin time

Zingiber officinale

Issues

Authors:

Ebrar Şeyma Özdemir | Sümeyye Koç | Demet Özçelik | Selman Aktaş | Mehmet Murat Yekrek | Esra Paydaş Hataysal | Mevlüt Sait Keleş | Fatih Gültekin | Eray Metin Güler

Publisher:

Istanbul University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

İstanbul Journal of Pharmacy Vol.54 Issue 3, pp.417-423

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001340659

pISSN:

2587-2087