Optimization of a Specific Real-Time PCR Assay for Fusarium avenaceum Using  Species-specific Couples of Primer

Şerife Evrim ARICI; Peter KARLOVSKY

Article Details

Optimization of a Specific Real-Time PCR Assay for Fusarium avenaceum Using Species-specific Couples of Primer

Authors:

Şerife Evrim ARICI | Peter KARLOVSKY

Publisher:

Türkiye Fitopatoloji Derneği

Journal:

The Journal of Turkish Phytopathology 2013 Vol.42 Issue 1-2-3, pp.1-34

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000802200

Frequency:

Tri-annual

pISSN:

0378-8024

Abstract:

 


Fusarium head blight (FHB)
is an important cereal disease and may result in the accumulation of toxins in
grains. Although F.
graminearum is globally the most prevalent FHB causing species, F. avenaceum is found in contaminated samples. Real-time PCR (qPCR) is the the standard analytical method for
species-specific, quantitative estimation of fungal biomass in the tissue of
host organisms. qPCR is useful for quantifying fungal colonization of crops
while distinguishing among species. Recently,
species-specific PCR primers have been developed for most Fusarium species that cause head blight. In some cases, the species-specific primers
synthesized with other Fusarium
species and can give the wrong results. In this study, two species-specific primer
pairs for F. avenaceum were tested by
using with different fungi DNA. JIA primer pairs were amplified only F. avenaceum, whereas MGA primer pairs
were amplified with F. equiseti and F. tricinctum. Results indicate that primer pair
JIA is effective in determination of F. avenaceum.

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Article Details

Optimization of a Specific Real-Time PCR Assay for Fusarium avenaceum Using Species-specific Couples of Primer

Authors: Şerife Evrim ARICI | Peter KARLOVSKY

Publisher: Türkiye Fitopatoloji Derneği

Journal: The Journal of Turkish Phytopathology 2013 Vol.42 Issue 1-2-3, pp.1-34

Document Type: Article

Publication Date: 5/2014

Document Language: Turkish

Document ID (Org No.): 2000802200

Frequency: Tri-annual

pISSN: 0378-8024

Issues

Authors:

Şerife Evrim ARICI | Peter KARLOVSKY

Publisher:

Türkiye Fitopatoloji Derneği

Journal:

The Journal of Turkish Phytopathology 2013 Vol.42 Issue 1-2-3, pp.1-34

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000802200

Frequency:

Tri-annual

pISSN:

0378-8024