In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa)

Mehmet Burak TURGUT; Muhammet Çağrı OĞUZ; Burak ÖNOL; Cengiz SANCAK

Article Details

In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa)

Authors:

Mehmet Burak TURGUT | Muhammet Çağrı OĞUZ | Burak ÖNOL | Cengiz SANCAK

Publisher:

Bitki Islahçıları Alt Birliği

Main Topics:

Agriculture

Journal:

Ekin Journal of Crop Breeding and Genetics 2022 Vol.8 Issue 2, pp.101-107

Document Type:

Article

Publication Date:

7/2022

Document Language:

English

Document ID (Org No.):

2000403665

eISSN:

2459-069X

pISSN:

2149-1275

Abstract:

 

Local populations and ecotypes are important genetic resources with genetic diversity. It is possible to protect threatened
genotypes and increase biodiversity with in vitro tissue culture techniques. Explant type is of great importance among the
factors affecting the success of in vitro regeneration and callus induction. In this study, Sainfoin seeds belonging to the Gözlü
ecotype were used as experiment material. MS media were prepared with TDZ and combinations of IBA-NAA. Callus
induction and shoot regeneration rates of hypocotyl, leaf and cotyledon explants were determined in the sainfoin ecotype.
According to the results of the study, the best callus formation and shoot regeneration were obtained from the hypocotyl
explant in an MS medium containing 2 mg/L TDZ. In the present study, important results were obtained that will contribute
to the regeneration studies of local ecotypes and wild species, genetic transformation and increasing genetic diversity.

Article Details

In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa)

Authors: Mehmet Burak TURGUT | Muhammet Çağrı OĞUZ | Burak ÖNOL | Cengiz SANCAK

Publisher: Bitki Islahçıları Alt Birliği

Main Topics: Agriculture

Journal: Ekin Journal of Crop Breeding and Genetics 2022 Vol.8 Issue 2, pp.101-107

Document Type: Article

Publication Date: 7/2022

Document Language: English

Document ID (Org No.): 2000403665

eISSN: 2459-069X

pISSN: 2149-1275

Issues

Authors:

Mehmet Burak TURGUT | Muhammet Çağrı OĞUZ | Burak ÖNOL | Cengiz SANCAK

Publisher:

Bitki Islahçıları Alt Birliği

Main Topics:

Agriculture

Journal:

Ekin Journal of Crop Breeding and Genetics 2022 Vol.8 Issue 2, pp.101-107

Document Type:

Article

Publication Date:

7/2022

Document Language:

English

Document ID (Org No.):

2000403665

eISSN:

2459-069X

pISSN:

2149-1275