Antioxidant Activities of the Extracts and Components of Teucrium orientale L. var.  orientale

Ahmet ÇAKIR; Ahmet MAVİ; Cavit KAZAZ; Ali YILDIRIM

Article Details

Antioxidant Activities of the Extracts and Components of Teucrium orientale L. var. orientale

Authors:

Ahmet ÇAKIR | Ahmet MAVİ | Cavit KAZAZ | Ali YILDIRIM

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2006 Vol.30 Issue 4, pp.483-494

Document Type:

Article

Publication Date:

3/2006

Document Language:

Turkish

Document ID (Org No.):

2000728871

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527

Abstract:

 

The effects of the developing stages of Teucrium orientale  L. var.  orientale on the antioxidant and DPPH radical scavenging  activities of the extracts obtained with different organic solvents  were investigated.  The aerial parts of the plant samples were  collected at the budding, flowering and vegetative stages and then the  plant samples were extracted separately with petroleum ether,  chloroform, acetone and methanol.  The antioxidant activities of the  extracts were evaluated using 2 different tests: the thiocyanate  method and scavenging of the 1,1-diphenyl-2-picrylhydrazyl (DPPH)  radical.  The acetone and methanol extracts of all of the harvesting  stages and chloroform extracts of the budding and flowering stages had  antioxidant activities.  Nevertheless, the acetone extracts of the  budding and flowering stages showed the highest antioxidant activity.   Higher DPPH radical scavenging activities were found in only acetone  and methanol extracts in all of the harvesting stages.  The flavonoid  contents of all of the extracts were also determined.  Similar to  antioxidant and DPPH radical scavenging activities, the amounts of  total flavonoids were higher in acetone and methanol extracts of the  flowering and budding stages.  The acetone extract of the flowering  stage, which contained the highest amount of total flavonoids, was  subjected to chromatographic methods for the isolation of the active  compounds.  Thus, a new iridoid along with 4 known flavonoids and 1  known iridoid were isolated.  The structures of isolated compounds  were characterized by UV, IR, 1H, 13C NMR, and 2D-NMR  spectroscopic methods as cirsilineol (1), luteolin-7-O-rutinoside (2),  luteolin-7-O-glucoside (3), hesperetin-7-O-rutinoside (4), 8-O-acetyl  harpagide (5) and 8-O-methyl harpagide (6).  These isolated compounds  were also tested for their antioxidant and DPPH radical scavenging  activities.  Flavonoids showed potent antioxidant and DPPH radical  scavenging activities, but iridoids did not.  The highest antioxidant  activity was shown by luteolin-7-O-glucoside.

 

The effects of the developing stages of Teucrium orientale  L. var.  orientale on the antioxidant and DPPH radical scavenging  activities of the extracts obtained with different organic solvents  were investigated.  The aerial parts of the plant samples were  collected at the budding, flowering and vegetative stages and then the  plant samples were extracted separately with petroleum ether,  chloroform, acetone and methanol.  The antioxidant activities of the  extracts were evaluated using 2 different tests: the thiocyanate  method and scavenging of the 1,1-diphenyl-2-picrylhydrazyl (DPPH)  radical.  The acetone and methanol extracts of all of the harvesting  stages and chloroform extracts of the budding and flowering stages had  antioxidant activities.  Nevertheless, the acetone extracts of the  budding and flowering stages showed the highest antioxidant activity.   Higher DPPH radical scavenging activities were found in only acetone  and methanol extracts in all of the harvesting stages.  The flavonoid  contents of all of the extracts were also determined.  Similar to  antioxidant and DPPH radical scavenging activities, the amounts of  total flavonoids were higher in acetone and methanol extracts of the  flowering and budding stages.  The acetone extract of the flowering  stage, which contained the highest amount of total flavonoids, was  subjected to chromatographic methods for the isolation of the active  compounds.  Thus, a new iridoid along with 4 known flavonoids and 1  known iridoid were isolated.  The structures of isolated compounds  were characterized by UV, IR, 1H, 13C NMR, and 2D-NMR  spectroscopic methods as cirsilineol (1), luteolin-7-O-rutinoside (2),  luteolin-7-O-glucoside (3), hesperetin-7-O-rutinoside (4), 8-O-acetyl  harpagide (5) and 8-O-methyl harpagide (6).  These isolated compounds  were also tested for their antioxidant and DPPH radical scavenging  activities.  Flavonoids showed potent antioxidant and DPPH radical  scavenging activities, but iridoids did not.  The highest antioxidant  activity was shown by luteolin-7-O-glucoside.

Article Details

Antioxidant Activities of the Extracts and Components of Teucrium orientale L. var. orientale

Authors: Ahmet ÇAKIR | Ahmet MAVİ | Cavit KAZAZ | Ali YILDIRIM

Publisher: TUBITAK

Journal: Turkish Journal of Chemistry 2006 Vol.30 Issue 4, pp.483-494

Document Type: Article

Publication Date: 3/2006

Document Language: Turkish

Document ID (Org No.): 2000728871

Frequency: Bimonthly

eISSN: 1303-6130

pISSN: 1300-0527

Issues

Authors:

Ahmet ÇAKIR | Ahmet MAVİ | Cavit KAZAZ | Ali YILDIRIM

Publisher:

TUBITAK

Journal:

Turkish Journal of Chemistry 2006 Vol.30 Issue 4, pp.483-494

Document Type:

Article

Publication Date:

3/2006

Document Language:

Turkish

Document ID (Org No.):

2000728871

Frequency:

Bimonthly

eISSN:

1303-6130

pISSN:

1300-0527