Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Ahmet ONAY

Article Details

Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Authors:

Ahmet ONAY

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Document Type:

Article

Publication Date:

3/2000

Document Language:

Turkish

Document ID (Org No.):

2000809778

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Keywords:

Abstract:

 

Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog  media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic mass-es  were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with 0.5-4 mg/l benzylaminop-urine.  After transfer of the embryogenic masses into the same medium, but after a few subcultures with benzylaminopurine, somat-ic  embryos appeared. Clusters of somatic embryos were transferred to the agar solidified MS medium for maturation. Matured  somatic embryos, germinated on the maturation medium without growth regulators, developed into plantlets.  Abbreviations: 2,4-D - 2,4-dichlorophenoxyacetic acid; BAP - benzylaminopurine (N 6 -benzyladenine); EMS -embryogenic mass; IAA  - Indole-3-acetic asid; MS - Murashige and Skoog medium; NAA - a- naphthalene acetic acid; PGR - plant growth regulator; SE(s) -somatic  embryo (s);.

 

Embryogenic mass was produced from kernels of mature fruits of Pistacia atlantica cultured in liquid Murashige and Skoog  media, supplemented with 100 mg/l casein hydrolysate, 100 mg/l l-ascorbic acid, and benzylaminopurine (BAP). Embryogenic mass-es  were differentiated directly from the kernel explants after culture for 3 weeks in liquid medium with 0.5-4 mg/l benzylaminop-urine.  After transfer of the embryogenic masses into the same medium, but after a few subcultures with benzylaminopurine, somat-ic  embryos appeared. Clusters of somatic embryos were transferred to the agar solidified MS medium for maturation. Matured  somatic embryos, germinated on the maturation medium without growth regulators, developed into plantlets.  Abbreviations: 2,4-D - 2,4-dichlorophenoxyacetic acid; BAP - benzylaminopurine (N 6 -benzyladenine); EMS -embryogenic mass; IAA  - Indole-3-acetic asid; MS - Murashige and Skoog medium; NAA - a- naphthalene acetic acid; PGR - plant growth regulator; SE(s) -somatic  embryo (s);.

Article Details

Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Authors:

Ahmet ONAY

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Document Type:

Article

Publication Date:

3/2000

Document Language:

Turkish

Document ID (Org No.):

2000809778

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Turk. J. Agric. For.

24

(2000)

465-474. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.24

iss.4.

Issues

Article Details

Somatic Embryogenesis From Mature Seed Cultures of Pistacia atlantica

Authors: Ahmet ONAY

Publisher: TUBITAK

Journal: Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Document Type: Article

Publication Date: 3/2000

Document Language: Turkish

Document ID (Org No.): 2000809778

Frequency: Bimonthly

eISSN: 1303-6173

pISSN: 1300-011X

Turk. J. Agric. For.

24

(2000)

465-474. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.24

iss.4.

Issues

Authors:

Ahmet ONAY

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2000 Vol.24 Issue 4, pp.465-474

Document Type:

Article

Publication Date:

3/2000

Document Language:

Turkish

Document ID (Org No.):

2000809778

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X