Effectiveness of Native Entomopathogenic Fungus Isolate Adapted to Low Humidity Conditions on Tenebrio molitor (Coleoptera:Tenebrionidae)

Mehmet Sedat Sevinç; Nuran Karatağ; Mesut Altındal

Article Details

Effectiveness of Native Entomopathogenic Fungus Isolate Adapted to Low Humidity Conditions on Tenebrio molitor (Coleoptera:Tenebrionidae)

Authors:

Mehmet Sedat Sevinç | Nuran Karatağ | Mesut Altındal

Publisher:

Harran University

Main Topics:

Tarımda Entomoloji

Journal:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 4, pp.593-603

Document Type:

Article

Publication Date:

12/2024

Document Language:

Turkish

Document ID (Org No.):

2001333307

pISSN:

2587-1358

Keywords:

Abstract:

 Especially for pest management in low humidity environments such as storage conditions, it is of great importance to obtain entomopathogenic fungal isolates that can maintain their virulence despite low humidity conditions. In this study; It was aimed to obtain enthomopathogenic fungus isolate that maintains its virulence at low soil moisture conditions using Tenebrio molitor from soil samples that have undergone standard soil analysis. In this context, fungal growth was observed in 4 soil samples without increasing the soil moisture at low relative humidity in 16 soil samples examined. As a result of morphological identification; It was observed that the isolates, which were found to be Beauveria bassiana, Cordyceps fumosorosea and two Metarhizium anisopliae species, caused 100 mortality on Tenebrio molitor larvae in petri dishes with bioassay studies. Beauveria bassiana isolate, on which mycelial development was observed on cadavers, was tested at 5 different spore densities in sterile soil conditions in plastic containers; On the 9th day; 108, 107, 106, 105, 104 conidia ml-1 doses were found to be 60, 23.34, 19.34, 12.15 and 14.17, respectively, while at the end of 18 days these rates were determined as 64, 43.34, 19.34, 15.72 and 14.17. The mortality rate in the control group was 0. It is thought that the use of the isolate obtained at 108 conidia ml-1 and higher densities has an important potential for other studies to be conducted in low humidity conditions such as food storage and that detailed studies are needed.

 Especially for pest management in low humidity environments such as storage conditions, it is of great importance to obtain entomopathogenic fungal isolates that can maintain their virulence despite low humidity conditions. In this study; It was aimed to obtain enthomopathogenic fungus isolate that maintains its virulence at low soil moisture conditions using Tenebrio molitor from soil samples that have undergone standard soil analysis. In this context, fungal growth was observed in 4 soil samples without increasing the soil moisture at low relative humidity in 16 soil samples examined. As a result of morphological identification; It was observed that the isolates, which were found to be Beauveria bassiana, Cordyceps fumosorosea and two Metarhizium anisopliae species, caused 100 mortality on Tenebrio molitor larvae in petri dishes with bioassay studies. Beauveria bassiana isolate, on which mycelial development was observed on cadavers, was tested at 5 different spore densities in sterile soil conditions in plastic containers; On the 9th day; 108, 107, 106, 105, 104 conidia ml-1 doses were found to be 60, 23.34, 19.34, 12.15 and 14.17, respectively, while at the end of 18 days these rates were determined as 64, 43.34, 19.34, 15.72 and 14.17. The mortality rate in the control group was 0. It is thought that the use of the isolate obtained at 108 conidia ml-1 and higher densities has an important potential for other studies to be conducted in low humidity conditions such as food storage and that detailed studies are needed.

 Especially for pest management in low humidity environments such as storage conditions, it is of great importance to obtain entomopathogenic fungal isolates that can maintain their virulence despite low humidity conditions. In this study; It was aimed to obtain enthomopathogenic fungus isolate that maintains its virulence at low soil moisture conditions using Tenebrio molitor from soil samples that have undergone standard soil analysis. In this context, fungal growth was observed in 4 soil samples without increasing the soil moisture at low relative humidity in 16 soil samples examined. As a result of morphological identification; It was observed that the isolates, which were found to be Beauveria bassiana, Cordyceps fumosorosea and two Metarhizium anisopliae species, caused 100 mortality on Tenebrio molitor larvae in petri dishes with bioassay studies. Beauveria bassiana isolate, on which mycelial development was observed on cadavers, was tested at 5 different spore densities in sterile soil conditions in plastic containers; On the 9th day; 108, 107, 106, 105, 104 conidia ml-1 doses were found to be 60, 23.34, 19.34, 12.15 and 14.17, respectively, while at the end of 18 days these rates were determined as 64, 43.34, 19.34, 15.72 and 14.17. The mortality rate in the control group was 0. It is thought that the use of the isolate obtained at 108 conidia ml-1 and higher densities has an important potential for other studies to be conducted in low humidity conditions such as food storage and that detailed studies are needed.

 Düşük nemli ortamlarda özellikle depolama koşullarında yapılacak olan mücadele için düşük nemde virülensliğini sürdüren fungus izolatları elde etmek önemlidir. Bu çalışmada; standart toprak analizi yapılmış toprak örneklerinin, Tenebrio molitor kullanılarak düşük toprak nemi koşullarında virülensliğini sürdüren entomopatojen fungus izolatı elde etmek amaçlanmıştır. Bu kapsamda incelenen 16 toprak örneğinde, düşük bağıl nemde toprak nemi artırılmadan 4 toprak örneğinde fungal gelişim görülmüştür. Morfolojik tanılama sonucu; Beauveria bassiana, Cordyceps fumosorosea ve iki adetinin de Metarhizium anisopliae türü olduğu görülen izolatların, Tenebrio molitor larvaları üzerinde petri kaplarında biyoassay çalışmaları ile 100 ölüme sebep olduğu görülmüştür. Kadavra üzerinde misel gelişimi görülen Beauveria bassiana izolatı, plastik kaplarda steril toprak koşullarında 5 farklı spor yoğunluğunda denenmiş; 108 , 107, 106, 105, 104 konidi ml-1 dozları 9. günde sırasıyla; 60, 23.34, 19.34, 12.15 ve 14.17 bulunurken, 18 gün sonunda ise bu oranlar; 64; 43.34; 19.34; 15.72 ve 14.17 olarak tespit edilmiştir. Kontrol grubunda ölüm oranı; 0 dır. Elde edilen izolatın 108 konidi ml-1 ve daha yüksek yoğunlukta kullanımının gıda depolama gibi düşük nem koşullarında yapılacak diğer çalışmalar için önemli bir potansiyeli olduğu ve detaylı çalışmalara ihtiyaç duyulduğu düşünülmektedir.

Article Details

Effectiveness of Native Entomopathogenic Fungus Isolate Adapted to Low Humidity Conditions on Tenebrio molitor (Coleoptera:Tenebrionidae)

Authors:

Mehmet Sedat Sevinç | Nuran Karatağ | Mesut Altındal

Publisher:

Harran University

Main Topics:

Tarımda Entomoloji

Journal:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 4, pp.593-603

Document Type:

Article

Publication Date:

12/2024

Document Language:

Turkish

Document ID (Org No.):

2001333307

pISSN:

2587-1358

Cordyceps

Beauveria

Metarhizium

Lethal effects

VirulenceCordyceps

Beauveria

Metarhizium

Ölümcül etkiler

Virülenslik

Issues

Article Details

Authors: Mehmet Sedat Sevinç | Nuran Karatağ | Mesut Altındal

Publisher: Harran University

Main Topics: Tarımda Entomoloji

Journal: Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 4, pp.593-603

Document Type: Article

Publication Date: 12/2024

Document Language: Turkish

Document ID (Org No.): 2001333307

pISSN: 2587-1358

Issues

Authors:

Mehmet Sedat Sevinç | Nuran Karatağ | Mesut Altındal

Publisher:

Harran University

Main Topics:

Tarımda Entomoloji

Journal:

Harran Tarım ve Gıda Bilimleri Dergisi Vol.28 Issue 4, pp.593-603

Document Type:

Article

Publication Date:

12/2024

Document Language:

Turkish

Document ID (Org No.):

2001333307

pISSN:

2587-1358