Investigation of Serotonin 2A Receptor Gene 5-HTR2A rs6311 Polymorphism in Trigeminal Neuralgia Disease

Firuze Çınar; Tolga Polat; Fatma Güngör; Beste Tacal Aslan; Gühan Dergin; Korkut Ulucan; Şehkar Oktay

Article Details

Investigation of Serotonin 2A Receptor Gene 5-HTR2A rs6311 Polymorphism in Trigeminal Neuralgia Disease

Authors:

Firuze Çınar | Tolga Polat | Fatma Güngör | Beste Tacal Aslan | Gühan Dergin | Korkut Ulucan | Şehkar Oktay

Publisher:

Marmara University

Main Topics:

Dentistry (Other)

Journal:

European Journal of Research in Dentistry Vol.8 Issue 1, pp.26-30

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001316551

pISSN:

2630-6247

Keywords:

Abstract:

 Objectives: Trigeminal neuralgia TN is a disease that occurs in the distribution region of the trigeminal nerve and is frequently recurrent pain. Serotonin 5-HT acts on neurotransmitters in the central nervous system. The study aims to examine the relationship between sensitivity and pain intensity of the serotonin receptor gene 5-HTR2A and rs6311 polymorphism in patients by examining TN genetically. Materials and Methods: The study consisted of 10 TN patients and 10 healthy individuals. Genotyping of 5-HTR2A rs6311 polymorphism using DNA isolated from blood, Real-Time PCR in StepOnePlus device, and Taqman SNP Genotyping Tests were performed by manufacturers protocols. Results: In 5HTR2A rs6311 analysis, 3 of 10 TN patients were found to have CC, 5 had CT and 2 had TT genotype. In the control group, CC genotype was detected in 4 individuals, CT genotype in 4 individuals, and TT genotype in 2 individuals. Conclusion: It was found that the TN genotype and allelic frequency differences between the patient and control groups were not significant. However, the CT genotype was found to be more common.

 Objectives: Trigeminal neuralgia TN is a disease that occurs in the distribution region of the trigeminal nerve and is frequently recurrent pain. Serotonin 5-HT acts on neurotransmitters in the central nervous system. The study aims to examine the relationship between sensitivity and pain intensity of the serotonin receptor gene 5-HTR2A and rs6311 polymorphism in patients by examining TN genetically. Materials and Methods: The study consisted of 10 TN patients and 10 healthy individuals. Genotyping of 5-HTR2A rs6311 polymorphism using DNA isolated from blood, Real-Time PCR in StepOnePlus device, and Taqman SNP Genotyping Tests were performed by manufacturers protocols. Results: In 5HTR2A rs6311 analysis, 3 of 10 TN patients were found to have CC, 5 had CT and 2 had TT genotype. In the control group, CC genotype was detected in 4 individuals, CT genotype in 4 individuals, and TT genotype in 2 individuals. Conclusion: It was found that the TN genotype and allelic frequency differences between the patient and control groups were not significant. However, the CT genotype was found to be more common.

 Objectives: Trigeminal neuralgia TN is a disease that occurs in the distribution region of the trigeminal nerve and is frequently recurrent pain. Serotonin 5-HT acts on neurotransmitters in the central nervous system. The study aims to examine the relationship between sensitivity and pain intensity of the serotonin receptor gene 5-HTR2A and rs6311 polymorphism in patients by examining TN genetically. Materials and Methods: The study consisted of 10 TN patients and 10 healthy individuals. Genotyping of 5-HTR2A rs6311 polymorphism using DNA isolated from blood, Real-Time PCR in StepOnePlus device, and Taqman SNP Genotyping Tests were performed by manufacturers protocols. Results: In 5HTR2A rs6311 analysis, 3 of 10 TN patients were found to have CC, 5 had CT and 2 had TT genotype. In the control group, CC genotype was detected in 4 individuals, CT genotype in 4 individuals, and TT genotype in 2 individuals. Conclusion: It was found that the TN genotype and allelic frequency differences between the patient and control groups were not significant. However, the CT genotype was found to be more common.

 Amaç: Trigeminal nevralji TN, trigeminal sinirin dağılım bölgesinde ortaya çıkan ve sıklıkla tekrarlayan ağrılarla karakterize bir hastalıktır. Serotonin 5-HT, merkezi sinir sistemindeki nörotransmitterlere etki eder. Çalışmada, TNyi genetik olarak inceleyerek hastalarda serotonin reseptör geni 5-HTR2A duyarlılığı ve ağrı şiddeti ile rs6311 polimorfizmi arasındaki ilişkiyi araştırmak amaçlanmıştır. Gereç ve Yöntem: Çalışmada 10 TN hastası ve 10 sağlıklı birey yer almıştır. Kanlardan izole edilen DNA, StepOnePlus cihazında Real-Time PCR ve Taqman SNP Genotipleme Testleri kullanılarak 5-HTR2A rs6311 polimorfizminin genotiplenmesi üretici protokollerine göre yapıldı. Bulgular: 5HTR2A rs6311 analizinde, 10 TN hastasından 3ünde CC, 5inde CT ve 2sinde TT genotipinin varlığı tespit edildi. Kontrol grubunda ise 4 kişide CC genotipi, 4 kişide CT genotipi ve 2 kişide TT genotipi tespit edildi. Sonuç: Hasta ve kontrol grupları arasındaki TN genotipi frekans farklılıklarının anlamlı olmadığı görüldü. Ancak CT genotipinin diğerlerine göre daha yaygın olduğu görüldü.

Article Details

Investigation of Serotonin 2A Receptor Gene 5-HTR2A rs6311 Polymorphism in Trigeminal Neuralgia Disease

Authors:

Firuze Çınar | Tolga Polat | Fatma Güngör | Beste Tacal Aslan | Gühan Dergin | Korkut Ulucan | Şehkar Oktay

Publisher:

Marmara University

Main Topics:

Dentistry (Other)

Journal:

European Journal of Research in Dentistry Vol.8 Issue 1, pp.26-30

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001316551

pISSN:

2630-6247

Trigeminal neuralgia

polymorphism

serotonin

serotonin receptorTrigeminal nevralji

polimorfizm

serotonin

serotonin reseptörü

Issues

Article Details

Authors: Firuze Çınar | Tolga Polat | Fatma Güngör | Beste Tacal Aslan | Gühan Dergin | Korkut Ulucan | Şehkar Oktay

Publisher: Marmara University

Main Topics: Dentistry (Other)

Journal: European Journal of Research in Dentistry Vol.8 Issue 1, pp.26-30

Document Type: Article

Publication Date: 4/2024

Document Language: English

Document ID (Org No.): 2001316551

pISSN: 2630-6247

Issues

Authors:

Firuze Çınar | Tolga Polat | Fatma Güngör | Beste Tacal Aslan | Gühan Dergin | Korkut Ulucan | Şehkar Oktay

Publisher:

Marmara University

Main Topics:

Dentistry (Other)

Journal:

European Journal of Research in Dentistry Vol.8 Issue 1, pp.26-30

Document Type:

Article

Publication Date:

4/2024

Document Language:

English

Document ID (Org No.):

2001316551

pISSN:

2630-6247