Investigation of the Protective Effects of Chrysin Against Paclitaxel-Induced Oxidative Stress and Apoptosis in Human Neuronal SH-SY5Y Cells

Adnan Ayna; Sema Nur Varan

Article Details

Investigation of the Protective Effects of Chrysin Against Paclitaxel-Induced Oxidative Stress and Apoptosis in Human Neuronal SH-SY5Y Cells

Authors:

Adnan Ayna | Sema Nur Varan

Publisher:

Bingol University

Main Topics:

Hücre Gelişimi | Proliferasyon ve Ölümü

Journal:

Türk Doğa ve Fen Dergisi Vol.12 Issue 4, pp.107-113

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001307731

pISSN:

2149-6366

Keywords:

Abstract:

 In this study, the potential protective effects of chrysin, an important flavonoid, against paclitaxel-induced cell toxicity in the SH-SY5Y nerve cell line as an in vitro model, were investigated by cell viability analysis, lipid peroxidation analysis and quantitative simultaneous PCR methods. In the study, firstly, paclitaxel and chrysin were applied to the SH-SY5Y cell line at different concentrations in the range of 0-30 µM, and the results showed that 15 and 30 µM paclitaxel reduced cell viability, and 500 and 1000 µM chrysin application reduced these effects. In addition, chrysin application has been shown to significantly reduce malondialdehyde levels in paclitaxel-induced cells. The study also examined the effects of paclitaxel and chrysin application on apoptotic and antiapoptotic genes, mostly located in the intrinsic pathway, and showed that chrysin significantly reduced the levels of caspase 10, caspase 8, caspase 6, p53 and NFKB, and increased the Bcl-2 level compared to the paclitaxel-treated group. The results of this study suggest that chrysins suppression of oxidative stress and apoptotic cell death may be an effective strategy for the treatment of paclitaxel-induced SH-SY5Y cytotoxicity.

 In this study, the potential protective effects of chrysin, an important flavonoid, against paclitaxel-induced cell toxicity in the SH-SY5Y nerve cell line as an in vitro model, were investigated by cell viability analysis, lipid peroxidation analysis and quantitative simultaneous PCR methods. In the study, firstly, paclitaxel and chrysin were applied to the SH-SY5Y cell line at different concentrations in the range of 0-30 µM, and the results showed that 15 and 30 µM paclitaxel reduced cell viability, and 500 and 1000 µM chrysin application reduced these effects. In addition, chrysin application has been shown to significantly reduce malondialdehyde levels in paclitaxel-induced cells. The study also examined the effects of paclitaxel and chrysin application on apoptotic and antiapoptotic genes, mostly located in the intrinsic pathway, and showed that chrysin significantly reduced the levels of caspase 10, caspase 8, caspase 6, p53 and NFKB, and increased the Bcl-2 level compared to the paclitaxel-treated group. The results of this study suggest that chrysins suppression of oxidative stress and apoptotic cell death may be an effective strategy for the treatment of paclitaxel-induced SH-SY5Y cytotoxicity.

 In this study, the potential protective effects of chrysin, an important flavonoid, against paclitaxel-induced cell toxicity in the SH-SY5Y nerve cell line as an in vitro model, were investigated by cell viability analysis, lipid peroxidation analysis and quantitative simultaneous PCR methods. In the study, firstly, paclitaxel and chrysin were applied to the SH-SY5Y cell line at different concentrations in the range of 0-30 µM, and the results showed that 15 and 30 µM paclitaxel reduced cell viability, and 500 and 1000 µM chrysin application reduced these effects. In addition, chrysin application has been shown to significantly reduce malondialdehyde levels in paclitaxel-induced cells. The study also examined the effects of paclitaxel and chrysin application on apoptotic and antiapoptotic genes, mostly located in the intrinsic pathway, and showed that chrysin significantly reduced the levels of caspase 10, caspase 8, caspase 6, p53 and NFKB, and increased the Bcl-2 level compared to the paclitaxel-treated group. The results of this study suggest that chrysins suppression of oxidative stress and apoptotic cell death may be an effective strategy for the treatment of paclitaxel-induced SH-SY5Y cytotoxicity.

 In this study, the potential protective effects of chrysin, an important flavonoid, against paclitaxel-induced cell toxicity in the SH-SY5Y nerve cell line as an in vitro model, were investigated by cell viability analysis, lipid peroxidation analysis and quantitative simultaneous PCR methods. In the study, firstly, paclitaxel and chrysin were applied to the SH-SY5Y cell line at different concentrations in the range of 0-30 µM, and the results showed that 15 and 30 µM paclitaxel reduced cell viability, and 500 and 1000 µM chrysin application reduced these effects. In addition, chrysin application has been shown to significantly reduce malondialdehyde levels in paclitaxel-induced cells. The study also examined the effects of paclitaxel and chrysin application on apoptotic and antiapoptotic genes, mostly located in the intrinsic pathway, and showed that chrysin significantly reduced the levels of caspase 10, caspase 8, caspase 6, p53 and NFKB, and increased the Bcl-2 level compared to the paclitaxel-treated group. The results of this study suggest that chrysins suppression of oxidative stress and apoptotic cell death may be an effective strategy for the treatment of paclitaxel-induced SH-SY5Y cytotoxicity.

Article Details

Investigation of the Protective Effects of Chrysin Against Paclitaxel-Induced Oxidative Stress and Apoptosis in Human Neuronal SH-SY5Y Cells

Authors:

Adnan Ayna | Sema Nur Varan

Publisher:

Bingol University

Main Topics:

Hücre Gelişimi | Proliferasyon ve Ölümü

Journal:

Türk Doğa ve Fen Dergisi Vol.12 Issue 4, pp.107-113

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001307731

pISSN:

2149-6366

Paclitaxel

chrysin

apoptosis

Bcl-2

malondialdehyde levels

Issues

Article Details

Investigation of the Protective Effects of Chrysin Against Paclitaxel-Induced Oxidative Stress and Apoptosis in Human Neuronal SH-SY5Y Cells

Authors: Adnan Ayna | Sema Nur Varan

Publisher: Bingol University

Main Topics: Hücre Gelişimi | Proliferasyon ve Ölümü

Journal: Türk Doğa ve Fen Dergisi Vol.12 Issue 4, pp.107-113

Document Type: Article

Publication Date: 12/2023

Document Language: English

Document ID (Org No.): 2001307731

pISSN: 2149-6366

Paclitaxel

chrysin

apoptosis

Bcl-2

malondialdehyde levels

Issues

Authors:

Adnan Ayna | Sema Nur Varan

Publisher:

Bingol University

Main Topics:

Hücre Gelişimi | Proliferasyon ve Ölümü

Journal:

Türk Doğa ve Fen Dergisi Vol.12 Issue 4, pp.107-113

Document Type:

Article

Publication Date:

12/2023

Document Language:

English

Document ID (Org No.):

2001307731

pISSN:

2149-6366