The Isolation and Characterization of Glucoamylase Enzyme of an  Aspergillus niger Natural Isolate

Gökhan CORAL

Article Details

The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

Authors:

Gökhan CORAL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.601-610

Document Type:

Article

Publication Date:

2/2000

Document Language:

Turkish

Document ID (Org No.):

2000814025

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural  isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source.  The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS  Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and  it was determined that the thermal stability of this enzyme is quite high.

 

An extracellular glucoamylase enzyme was isolated from Aspergillus niger Z10 natural  isolate in a liquid Czapek Dox medium which contained soluble starch (2%) as the sole carbon source.  The molecular weight of the enzyme was found to be about 100.000 Daltons through Starch-SDS  Polyacrylamide Gel Electrophoresis. The optimal pH range of the enzyme was found to be 4.5-5.5 and  it was determined that the thermal stability of this enzyme is quite high.

Article Details

The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

Authors:

Gökhan CORAL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.601-610

Document Type:

Article

Publication Date:

2/2000

Document Language:

Turkish

Document ID (Org No.):

2000814025

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Aspergillus niger

Glucoamylase.

Issues

Article Details

The Isolation and Characterization of Glucoamylase Enzyme of an Aspergillus niger Natural Isolate

Authors: Gökhan CORAL

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.601-610

Document Type: Article

Publication Date: 2/2000

Document Language: Turkish

Document ID (Org No.): 2000814025

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Aspergillus niger

Glucoamylase.

Issues

Authors:

Gökhan CORAL

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.601-610

Document Type:

Article

Publication Date:

2/2000

Document Language:

Turkish

Document ID (Org No.):

2000814025

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152