In Vitro Mass Propagation of Cucumis sativus L. from Nodal Segments

Naseem AHMAD; Mohammad ANIS

Article Details

In Vitro Mass Propagation of Cucumis sativus L. from Nodal Segments

Authors:

Naseem AHMAD | Mohammad ANIS

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2005 Vol.29 Issue 3, pp.237-240

Document Type:

Article

Publication Date:

2/2005

Document Language:

Turkish

Document ID (Org No.):

2000724028

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X

Abstract:

 

An efficient reproducible protocol for the in vitro multiplication of cucumber (Cucumis sativus L.), an important vegetable crop, was developed from nodal explants. Addition of casein hydrolysate to the shoot induction medium (MS + BA) significantly enhanced the number of multiple shoots or growth of the regenerants. Optimum shoot regeneration was observed on Murashige and Skoog (MS) medium containing 1.0 µM 6-benzyladenine (BA) and (200 mg/l) casein hydrolysate. Rooting of isolated in vitro raised microshoots was readily achieved with (1.0 µM) a-naphthalene-acetic acid (NAA) in 1/2 MS. The plantlets thus obtained were successfully established in a greenhouse.

 

An efficient reproducible protocol for the in vitro multiplication of cucumber (Cucumis sativus L.), an important vegetable crop, was developed from nodal explants. Addition of casein hydrolysate to the shoot induction medium (MS + BA) significantly enhanced the number of multiple shoots or growth of the regenerants. Optimum shoot regeneration was observed on Murashige and Skoog (MS) medium containing 1.0 µM 6-benzyladenine (BA) and (200 mg/l) casein hydrolysate. Rooting of isolated in vitro raised microshoots was readily achieved with (1.0 µM) a-naphthalene-acetic acid (NAA) in 1/2 MS. The plantlets thus obtained were successfully established in a greenhouse.

Article Details

In Vitro Mass Propagation of Cucumis sativus L. from Nodal Segments

Authors: Naseem AHMAD | Mohammad ANIS

Publisher: TUBITAK

Journal: Turkish Journal of Botany 2005 Vol.29 Issue 3, pp.237-240

Document Type: Article

Publication Date: 2/2005

Document Language: Turkish

Document ID (Org No.): 2000724028

Frequency: Bimonthly

eISSN: 1303-6106

pISSN: 1300-008X

Issues

Authors:

Naseem AHMAD | Mohammad ANIS

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2005 Vol.29 Issue 3, pp.237-240

Document Type:

Article

Publication Date:

2/2005

Document Language:

Turkish

Document ID (Org No.):

2000724028

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X