ANTITUMOUR   ACTIVITY OF Ganoderma mbrekobenum ON MONOSODIUM GLUTAMATE INDUCED UTERINE TUMOUR IN WISTAR RATS (Rattus norvegicus)

Lauretta Ofodile; Viola Nicholas-okpara

Article Details

ANTITUMOUR ACTIVITY OF Ganoderma mbrekobenum ON MONOSODIUM GLUTAMATE INDUCED UTERINE TUMOUR IN WISTAR RATS (Rattus norvegicus)

Authors:

Lauretta Ofodile | Viola Nicholas-okpara

Publisher:

Yozgat Bozok University

Main Topics:

Analitik Biyokimya

Journal:

Bozok Journal of Science Vol.3 Issue 1, pp.20-33

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001349270

pISSN:

2980-3314

Keywords:

Abstract:

 Ganoderma species is widely reported to be anticancer and antitumour but there seems to be no report on indigenous species against uterine tumours. Monosodium glutamate MSG has been shown to induce uterine tumours in high concentrations. Antitumour effect of the water extract of a Ganoderma species on Wistar rats given MSG was conducted. Wistar rats were fed with monosodium glutamate MSG to induce uterine tumour in the animals and were treated with water extract of the Ganoderma mbrekobenum. Heamatological, biochemical, histopathological examinations and acute toxicity test LD50 were done on the animals. The heart and liver in groups fed with MSG had significant increase in weight but the groups treated with G. mbrekobenum reduced in weight. The MSG-treated group showed indications of anaemia and toxicity in the animals. Renal functions and lipid profiles of the rats were fairly altered while the hepatic functions were significantly p 0.05 altered. Also, the amount of estrogen in the rats was elevated. Animals exposed to G. mbrekobenum showed signs of recovery from the effects of MSG in the rats. The acute toxicity test showed no fatalities among the animals and the extract of the G. mbrekobenum was notably lowered the levels of estrogen in the rats. The histological experiment revealed the presence of growthscysts in the uterine tissues of rats fed with MSG alone, whereas the rats treated with G. mbrekobenum 800mgkgbody weight exhibited no histopathological alterations. These findings indicate that the G. mbrekobenum could prevent and control uterine tumour in Wistar rats.

 Ganoderma species is widely reported to be anticancer and antitumour but there seems to be no report on indigenous species against uterine tumours. Monosodium glutamate MSG has been shown to induce uterine tumours in high concentrations. Antitumour effect of the water extract of a Ganoderma species on Wistar rats given MSG was conducted. Wistar rats were fed with monosodium glutamate MSG to induce uterine tumour in the animals and were treated with water extract of the Ganoderma mbrekobenum. Heamatological, biochemical, histopathological examinations and acute toxicity test LD50 were done on the animals. The heart and liver in groups fed with MSG had significant increase in weight but the groups treated with G. mbrekobenum reduced in weight. The MSG-treated group showed indications of anaemia and toxicity in the animals. Renal functions and lipid profiles of the rats were fairly altered while the hepatic functions were significantly p 0.05 altered. Also, the amount of estrogen in the rats was elevated. Animals exposed to G. mbrekobenum showed signs of recovery from the effects of MSG in the rats. The acute toxicity test showed no fatalities among the animals and the extract of the G. mbrekobenum was notably lowered the levels of estrogen in the rats. The histological experiment revealed the presence of growthscysts in the uterine tissues of rats fed with MSG alone, whereas the rats treated with G. mbrekobenum 800mgkgbody weight exhibited no histopathological alterations. These findings indicate that the G. mbrekobenum could prevent and control uterine tumour in Wistar rats.

 Ganoderma species is widely reported to be anticancer and antitumour but there seems to be no report on indigenous species against uterine tumours. Monosodium glutamate MSG has been shown to induce uterine tumours in high concentrations. Antitumour effect of the water extract of a Ganoderma species on Wistar rats given MSG was conducted. Wistar rats were fed with monosodium glutamate MSG to induce uterine tumour in the animals and were treated with water extract of the Ganoderma mbrekobenum. Heamatological, biochemical, histopathological examinations and acute toxicity test LD50 were done on the animals. The heart and liver in groups fed with MSG had significant increase in weight but the groups treated with G. mbrekobenum reduced in weight. The MSG-treated group showed indications of anaemia and toxicity in the animals. Renal functions and lipid profiles of the rats were fairly altered while the hepatic functions were significantly p 0.05 altered. Also, the amount of estrogen in the rats was elevated. Animals exposed to G. mbrekobenum showed signs of recovery from the effects of MSG in the rats. The acute toxicity test showed no fatalities among the animals and the extract of the G. mbrekobenum was notably lowered the levels of estrogen in the rats. The histological experiment revealed the presence of growthscysts in the uterine tissues of rats fed with MSG alone, whereas the rats treated with G. mbrekobenum 800mgkgbody weight exhibited no histopathological alterations. These findings indicate that the G. mbrekobenum could prevent and control uterine tumour in Wistar rats.

 Mantar, Ganoderma türünün yaygın olarak antikanser ve antitümör olduğu bildirilmektedir, ancak rahim tümörleri hakkında yerli türler hakkında bir rapor yok gibi görünmektedir. Monosodyum glutamatın MSG yüksek konsantrasyonlarda rahim tümörlerini indüklediği gösterilmiştir. Wistar cinsi sıçanlarda bir Ganoderma türünün sulu ekstraktının MSG verilen antitümör sonuçları elde edildi. Wistar sıçanları, hayvanlarda uterus tümörünü indüklemek için monosodyum glutamat MSG ile beslendi ve Ganoderma mbrekobenumun su özütü ile muamele edildi. Sıçanlara hematolojik, biyokimyasal, histopatolojik incelemeler yapıldı ve akut toksisite testi LD50 yapıldı. Sonuçlar, tedavi edilen tüm gruplardaki sıçanlarda kilo alımının gözlendiğini göstermiştir. Böbrek ve uterus, ağırlığı artan grup 4 ve 6 dışındaki tüm gruplarda kilo almadı. MSG ile beslenen gruplarda kalp ve karaciğer ağırlığında anlamlı bir artış vardı, ancak G. mbrekobenum ile tedavi edilen grupların ağırlığı azaldı. Monosodyum glutamat ile tedavi edilen grup, hayvanlarda anemi ve toksisite belirtileri gösterdi. Sıçanların böbrek fonksiyonları ve lipid profilleri oldukça değişirken, karaciğer fonksiyonları önemli ölçüde p 0.05 değişti. Ayrıca, sıçanlarda östrojen seviyesi yükseldi. G. mbrekobenum ile tedavi edilen hayvanlar, sıçanlarda MSGnin etkilerinden iyileşme belirtileri gösterdi. Akut toksisite testi, hayvanların ölümünü kaydetmedi ve G. mbrekobenum özütünün, hem MSG hem de G. mbrekobenum özütü ile beslendikten sonra sıçanların östrojen seviyelerini kontrole kıyasla önemli ölçüde düşürdüğü bulundu. Histolojik incelemede sadece MSG ile beslenen sıçanların uterus dokularında büyümekist varlığı görülürken, G. mbrekobenum ve monosodyum glutamat 800mgkgvücut ağırlığı ile beslenen sıçanlarda histopatolojik değişiklik belirtisi görülmedi. Yukarıdaki sonuçlar, G. mbrekobenumun Wistar sıçanlarında uterus tümörünü önleyebileceğini ve kontrol edebileceğini göstermektedir.

Article Details

ANTITUMOUR ACTIVITY OF Ganoderma mbrekobenum ON MONOSODIUM GLUTAMATE INDUCED UTERINE TUMOUR IN WISTAR RATS (Rattus norvegicus)

Authors:

Lauretta Ofodile | Viola Nicholas-okpara

Publisher:

Yozgat Bozok University

Main Topics:

Analitik Biyokimya

Journal:

Bozok Journal of Science Vol.3 Issue 1, pp.20-33

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001349270

pISSN:

2980-3314

Antitumour

Ganoderma

Monosodium glutamate

and Wistar ratsAnahtar Kelimeler Anahtar Kelime: Tümör, Monosodyum glutamat, Ganoderma türleri, Toksisite ve Hematoloji

Issues

Article Details

ANTITUMOUR ACTIVITY OF Ganoderma mbrekobenum ON MONOSODIUM GLUTAMATE INDUCED UTERINE TUMOUR IN WISTAR RATS (Rattus norvegicus)

Authors: Lauretta Ofodile | Viola Nicholas-okpara

Publisher: Yozgat Bozok University

Main Topics: Analitik Biyokimya

Journal: Bozok Journal of Science Vol.3 Issue 1, pp.20-33

Document Type: Article

Publication Date: 5/2025

Document Language: English

Document ID (Org No.): 2001349270

pISSN: 2980-3314

Antitumour

Ganoderma

Monosodium glutamate

and Wistar ratsAnahtar Kelimeler Anahtar Kelime: Tümör, Monosodyum glutamat, Ganoderma türleri, Toksisite ve Hematoloji

Issues

Authors:

Lauretta Ofodile | Viola Nicholas-okpara

Publisher:

Yozgat Bozok University

Main Topics:

Analitik Biyokimya

Journal:

Bozok Journal of Science Vol.3 Issue 1, pp.20-33

Document Type:

Article

Publication Date:

5/2025

Document Language:

English

Document ID (Org No.):

2001349270

pISSN:

2980-3314