Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Azra Zahrah Nadhirah Ikhwani; Idris Idris; Rizki Rabeca Elfirta; Pamungkas Rizki Ferdian

Article Details

Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Authors:

Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Publisher:

Turkish Chemical Society

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001309563

pISSN:

2149-0120

Keywords:

Abstract:

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

 Lipases are widely used in a variety of industries, both to develop products and to improve process efficiency. The need for lipase increased along with the wider application of this enzyme. Therefore, studies related to the search for potential lipase-producing microbes that answer the needs of the industry are required to be carried out continuously. Enzymes produced by microbes are preferred because they can be produced quickly compared to other sources. Bacillus flexus InaCC-B486 was used to produce lipase in this study with olive oil as substrate. This research aimed to observe the production of lipase from B. flexus InaCC-B486 and characterize its activity. The result shows that the production of B. flexus InaCC-B486 lipase was optimal at day-4 which was 11.983  0.101 UmL. The activity of B. flexus InaCC-B486 lipase was optimal at an incubation time of 15 minutes 2.810 UmL, pH of 8.0 3.173 UmL, and a temperature of 35 oC 3.173 UmL. These findings can be used for further applications, both in research and industry, that use B. flexus InaCC-B486 as a resource for lipase production or any related applications.

Article Details

Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Authors:

Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Publisher:

Turkish Chemical Society

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001309563

pISSN:

2149-0120

Bacillus flexus InaCC-B486

lipase enzyme

production optimization.

Issues

Article Details

Production and Activity Characterization of Lipase from Bacillus flexus InaCC-B486

Authors: Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Publisher: Turkish Chemical Society

Main Topics: Biochemistry and Cell Biology (Other)

Journal: Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Document Type: Article

Publication Date: 5/2024

Document Language: English

Document ID (Org No.): 2001309563

pISSN: 2149-0120

Bacillus flexus InaCC-B486

lipase enzyme

production optimization.

Issues

Authors:

Azra Zahrah Nadhirah Ikhwani | Idris Idris | Rizki Rabeca Elfirta | Pamungkas Rizki Ferdian

Publisher:

Turkish Chemical Society

Main Topics:

Biochemistry and Cell Biology (Other)

Journal:

Journal of the Turkish Chemical Society Section A: Chemistry Vol.11 Issue 2, pp.397-404

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001309563

pISSN:

2149-0120