Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells

Hakan ÇİFTÇİ; Mustafa TÜRK; Uğur TAMER; Siyami KARAHAN; Yusuf MENEMEN

doi:https://doi.org/10.3906/biy-1302-21

Article Details

Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells

Authors:

Hakan ÇİFTÇİ | Mustafa TÜRK | Uğur TAMER | Siyami KARAHAN | Yusuf MENEMEN

Publisher:

TUBITAK

Main Topics:

Life sciences

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.573-581

Document Type:

Article

Publication Date:

4/2013

Document Language:

English

Document ID (Org No.):

2000812364

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1302-21

Keywords:

Abstract:

 

The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-V-FLUOS immunostaining and double staining of Hoechst dye with propidium iodide. In AFM and TEM analyses, the sizes of AgNPs varied from 16 nm to 20 nm. AgNPs were 80 nm in hydrodynamic diameter with a zeta potential of 38.2 mV. The WST-1 assay resulted in an IC50 value of 40 µg/mL. AgNPs caused apoptotic and necrotic effects in a dose-dependent manner. The apoptotic effect of AgNPs was marked up to a concentration of 80 µg/mL AgNPs. At higher concentrations, the apoptotic effect decreased while the necrotic effect became prominent. The results indicate that AgNPs with a zeta potential of 38.2 mV and hydrodynamic diameter of 80 nm can be used in vitro at concentrations of up to 40 µg/mL.

 

The present study was conducted to examine cytotoxic, apoptotic, and necrotic effects of silver nanoparticles (AgNPs) on MCF-7 cells. Colloidal AgNPs were fabricated in an alkaline pH environment via reduction of silver nitrate with hydroxylamine hydrochloride. The size of AgNPs was measured by atomic force microscopy (AFM), transmission electron microscopy (TEM), and dynamic light scattering. Zeta potential of AgNPs was determined by laser Doppler microelectrophoresis. After exposing MCF-7 cells to AgNPs for 24 h, cytotoxicity was measured by WST-1 assay. Apoptosis and necrosis in MCF-7 cells were detected by Annexin-V-FLUOS immunostaining and double staining of Hoechst dye with propidium iodide. In AFM and TEM analyses, the sizes of AgNPs varied from 16 nm to 20 nm. AgNPs were 80 nm in hydrodynamic diameter with a zeta potential of 38.2 mV. The WST-1 assay resulted in an IC50 value of 40 µg/mL. AgNPs caused apoptotic and necrotic effects in a dose-dependent manner. The apoptotic effect of AgNPs was marked up to a concentration of 80 µg/mL AgNPs. At higher concentrations, the apoptotic effect decreased while the necrotic effect became prominent. The results indicate that AgNPs with a zeta potential of 38.2 mV and hydrodynamic diameter of 80 nm can be used in vitro at concentrations of up to 40 µg/mL.

Article Details

Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells

Authors:

Hakan ÇİFTÇİ | Mustafa TÜRK | Uğur TAMER | Siyami KARAHAN | Yusuf MENEMEN

Publisher:

TUBITAK

Main Topics:

Life sciences

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.573-581

Document Type:

Article

Publication Date:

4/2013

Document Language:

English

Document ID (Org No.):

2000812364

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1302-21

Key words: Silver

nanoparticle

MCF-7

cancer

apoptosis

necrosis

cytotoxicity

Issues

Article Details

Authors: Hakan ÇİFTÇİ | Mustafa TÜRK | Uğur TAMER | Siyami KARAHAN | Yusuf MENEMEN

Publisher: TUBITAK

Main Topics: Life sciences

Journal: Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.573-581

Document Type: Article

Publication Date: 4/2013

Document Language: English

Document ID (Org No.): 2000812364

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-1302-21

Issues

Authors:

Hakan ÇİFTÇİ | Mustafa TÜRK | Uğur TAMER | Siyami KARAHAN | Yusuf MENEMEN

Publisher:

TUBITAK

Main Topics:

Life sciences

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 5, pp.573-581

Document Type:

Article

Publication Date:

4/2013

Document Language:

English

Document ID (Org No.):

2000812364

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1302-21