Biodiversity of Actinobacteria Isolated from Marmara and Avşa Islands in Türkiye

Ahmet Rıdvan Topkara; Kamil Işık

Article Details

Biodiversity of Actinobacteria Isolated from Marmara and Avşa Islands in Türkiye

Authors:

Ahmet Rıdvan Topkara | Kamil Işık

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Bakteriyoloji

Journal:

Black Sea Journal of Engineering and Science Vol.6 Issue 4, pp.502-521

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001299854

pISSN:

2619-8991

Keywords:

Abstract:

 The main purpose of this study is to investigate the microbial diversity of Actinobacteria, living in soils in the Marmara and Avşa Islands and to identify species at genus level based on 16S rRNA gene sequences. These islands are located in the southwest of the Sea of Marmara and in the literature review, no Actinobacteria biodiversity studies related to Marmara and Avşa Islands were found. Such unexplored ecological habitats are potentially rich source for discovery sources of novel species and bioactive molecule. Actinobacteria play an important role in many natural phenomena such as nitrogen fixation, roles of these prokaryotic microorganisms. In this article, we focused on the presence and the diversity of Actinobacteria on the Islands by examining multiple sampling sites and using different selective isolation media. A total of 400 culturable Actinobacteria were isolated using ten different isolation media by dilution-plating method. Among the 400 isolates, 112 isolates were selected according to their morphology in different culture media. The isolates were characterized on the basis of 16S ribosomal RNA gene sequencing and phylogenetic analysis. The results showed a high level of actinobacterial diversity with 16 different genera. These genera obtained as a result of phylogenetic analyzes are Streptomyces, Nonomuraea, Nocardia, Actinomadura, Micromonospora, Kribbella, Mycolicibacterium, Microbispora, Saccharopolyspora, Jiangella, Rhodococcus, Actinopolymorpha, Geodermatophilus, Dactylosporangium, Pseudonocardia and Nocardioides. Many isolates are identified as new species by our current research. Findings from this study showed that the soil of Marmara and Avşa Islands can be a good source of isolation for Actinobacteria.

 The main purpose of this study is to investigate the microbial diversity of Actinobacteria, living in soils in the Marmara and Avşa Islands and to identify species at genus level based on 16S rRNA gene sequences. These islands are located in the southwest of the Sea of Marmara and in the literature review, no Actinobacteria biodiversity studies related to Marmara and Avşa Islands were found. Such unexplored ecological habitats are potentially rich source for discovery sources of novel species and bioactive molecule. Actinobacteria play an important role in many natural phenomena such as nitrogen fixation, roles of these prokaryotic microorganisms. In this article, we focused on the presence and the diversity of Actinobacteria on the Islands by examining multiple sampling sites and using different selective isolation media. A total of 400 culturable Actinobacteria were isolated using ten different isolation media by dilution-plating method. Among the 400 isolates, 112 isolates were selected according to their morphology in different culture media. The isolates were characterized on the basis of 16S ribosomal RNA gene sequencing and phylogenetic analysis. The results showed a high level of actinobacterial diversity with 16 different genera. These genera obtained as a result of phylogenetic analyzes are Streptomyces, Nonomuraea, Nocardia, Actinomadura, Micromonospora, Kribbella, Mycolicibacterium, Microbispora, Saccharopolyspora, Jiangella, Rhodococcus, Actinopolymorpha, Geodermatophilus, Dactylosporangium, Pseudonocardia and Nocardioides. Many isolates are identified as new species by our current research. Findings from this study showed that the soil of Marmara and Avşa Islands can be a good source of isolation for Actinobacteria.

 The main purpose of this study is to investigate the microbial diversity of Actinobacteria, living in soils in the Marmara and Avşa Islands and to identify species at genus level based on 16S rRNA gene sequences. These islands are located in the southwest of the Sea of Marmara and in the literature review, no Actinobacteria biodiversity studies related to Marmara and Avşa Islands were found. Such unexplored ecological habitats are potentially rich source for discovery sources of novel species and bioactive molecule. Actinobacteria play an important role in many natural phenomena such as nitrogen fixation, roles of these prokaryotic microorganisms. In this article, we focused on the presence and the diversity of Actinobacteria on the Islands by examining multiple sampling sites and using different selective isolation media. A total of 400 culturable Actinobacteria were isolated using ten different isolation media by dilution-plating method. Among the 400 isolates, 112 isolates were selected according to their morphology in different culture media. The isolates were characterized on the basis of 16S ribosomal RNA gene sequencing and phylogenetic analysis. The results showed a high level of actinobacterial diversity with 16 different genera. These genera obtained as a result of phylogenetic analyzes are Streptomyces, Nonomuraea, Nocardia, Actinomadura, Micromonospora, Kribbella, Mycolicibacterium, Microbispora, Saccharopolyspora, Jiangella, Rhodococcus, Actinopolymorpha, Geodermatophilus, Dactylosporangium, Pseudonocardia and Nocardioides. Many isolates are identified as new species by our current research. Findings from this study showed that the soil of Marmara and Avşa Islands can be a good source of isolation for Actinobacteria.

 The main purpose of this study is to investigate the microbial diversity of Actinobacteria, living in soils in the Marmara and Avşa Islands and to identify species at genus level based on 16S rRNA gene sequences. These islands are located in the southwest of the Sea of Marmara and in the literature review, no Actinobacteria biodiversity studies related to Marmara and Avşa Islands were found. Such unexplored ecological habitats are potentially rich source for discovery sources of novel species and bioactive molecule. Actinobacteria play an important role in many natural phenomena such as nitrogen fixation, roles of these prokaryotic microorganisms. In this article, we focused on the presence and the diversity of Actinobacteria on the Islands by examining multiple sampling sites and using different selective isolation media. A total of 400 culturable Actinobacteria were isolated using ten different isolation media by dilution-plating method. Among the 400 isolates, 112 isolates were selected according to their morphology in different culture media. The isolates were characterized on the basis of 16S ribosomal RNA gene sequencing and phylogenetic analysis. The results showed a high level of actinobacterial diversity with 16 different genera. These genera obtained as a result of phylogenetic analyzes are Streptomyces, Nonomuraea, Nocardia, Actinomadura, Micromonospora, Kribbella, Mycolicibacterium, Microbispora, Saccharopolyspora, Jiangella, Rhodococcus, Actinopolymorpha, Geodermatophilus, Dactylosporangium, Pseudonocardia and Nocardioides. Many isolates are identified as new species by our current research. Findings from this study showed that the soil of Marmara and Avşa Islands can be a good source of isolation for Actinobacteria.

Article Details

Biodiversity of Actinobacteria Isolated from Marmara and Avşa Islands in Türkiye

Authors:

Ahmet Rıdvan Topkara | Kamil Işık

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Bakteriyoloji

Journal:

Black Sea Journal of Engineering and Science Vol.6 Issue 4, pp.502-521

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001299854

pISSN:

2619-8991

Actinobacteria

Isolation

16S rRNA gene

Island soil

BiodiversityActinobacteria

Isolation

16S rRNA gene

Island soil

Biodiversity

Issues

Article Details

Authors: Ahmet Rıdvan Topkara | Kamil Işık

Publisher: Karyay Karadeniz Yayımcılık

Main Topics: Bakteriyoloji

Journal: Black Sea Journal of Engineering and Science Vol.6 Issue 4, pp.502-521

Document Type: Article

Publication Date: 10/2023

Document Language: English

Document ID (Org No.): 2001299854

pISSN: 2619-8991

Issues

Authors:

Ahmet Rıdvan Topkara | Kamil Işık

Publisher:

Karyay Karadeniz Yayımcılık

Main Topics:

Bakteriyoloji

Journal:

Black Sea Journal of Engineering and Science Vol.6 Issue 4, pp.502-521

Document Type:

Article

Publication Date:

10/2023

Document Language:

English

Document ID (Org No.):

2001299854

pISSN:

2619-8991