The Use of HPLC-DAD Method in Determining the Quality of Honeys: 5-Hydroxymethylfurfural (HMF) Analysis

Merve Özbay; Fatma Nur Arslan; Gazi Görür

Article Details

The Use of HPLC-DAD Method in Determining the Quality of Honeys: 5-Hydroxymethylfurfural (HMF) Analysis

Authors:

Merve Özbay | Fatma Nur Arslan | Gazi Görür

Publisher:

Karamanoglu Mehmetbey University

Main Topics:

Analitik Kimya (Diğer) | Gıda Mühendisliği

Journal:

Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 2, pp.41-45

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001325032

pISSN:

2687-5071

Keywords:

Abstract:

 The recognition of 5-hydroxymethylfurfural HMF content in honeys has been the subject of many scientific studies as it is a significant indicator of the toxicity, its processing method, storage situations and age. In this research, the amount of HMF in 95 different flower and honeydew honey samples obtained from markets, factory sales offices and local beekeepers in Turkiye was determined. High performance liquid chromatography HPLC method was employed to recognize HMF contents. Before sample analysis, the standard HMF solutions were prepared at different concentrations and method validation studies were carried out. The HMF content limit value of flower and honeydew honey in the Honey Communiqué TFCCommuniqué No:20207 is stated as 40 mg.kg-1. According to the obtained results, the HMF contents in honey samples were found as 0.00-16.65 mg.kg-1. It was determined that the amounts of HMF were at the highest level in monofloral milk vetch honey MH_MV samples coded MH_MV3 and MH_MV4. Therefore, it was concluded that all of the honey samples were compatible with the Honey Communiqué in terms of HMF and had appropriate honey quality values.

 The recognition of 5-hydroxymethylfurfural HMF content in honeys has been the subject of many scientific studies as it is a significant indicator of the toxicity, its processing method, storage situations and age. In this research, the amount of HMF in 95 different flower and honeydew honey samples obtained from markets, factory sales offices and local beekeepers in Turkiye was determined. High performance liquid chromatography HPLC method was employed to recognize HMF contents. Before sample analysis, the standard HMF solutions were prepared at different concentrations and method validation studies were carried out. The HMF content limit value of flower and honeydew honey in the Honey Communiqué TFCCommuniqué No:20207 is stated as 40 mg.kg-1. According to the obtained results, the HMF contents in honey samples were found as 0.00-16.65 mg.kg-1. It was determined that the amounts of HMF were at the highest level in monofloral milk vetch honey MH_MV samples coded MH_MV3 and MH_MV4. Therefore, it was concluded that all of the honey samples were compatible with the Honey Communiqué in terms of HMF and had appropriate honey quality values.

 The recognition of 5-hydroxymethylfurfural HMF content in honeys has been the subject of many scientific studies as it is a significant indicator of the toxicity, its processing method, storage situations and age. In this research, the amount of HMF in 95 different flower and honeydew honey samples obtained from markets, factory sales offices and local beekeepers in Turkiye was determined. High performance liquid chromatography HPLC method was employed to recognize HMF contents. Before sample analysis, the standard HMF solutions were prepared at different concentrations and method validation studies were carried out. The HMF content limit value of flower and honeydew honey in the Honey Communiqué TFCCommuniqué No:20207 is stated as 40 mg.kg-1. According to the obtained results, the HMF contents in honey samples were found as 0.00-16.65 mg.kg-1. It was determined that the amounts of HMF were at the highest level in monofloral milk vetch honey MH_MV samples coded MH_MV3 and MH_MV4. Therefore, it was concluded that all of the honey samples were compatible with the Honey Communiqué in terms of HMF and had appropriate honey quality values.

 Baldaki 5-hidroksimetilfurfural HMF içeriğinin tespiti balın toksititesinin, işlenme şeklinin, saklama koşullarının ve yaşının önemli bir göstergesi olduğu için birçok bilimsel araştırmanın konusu olmuştur. Bu çalışmada Türkiyedeki marketlerden, fabrika satış ofislerinden ve yerel bal üreticilerinden temin edilen 95 farklı çiçek ve salgı balı numunesinin HMF içeriği belirlenmiştir. HMF içeriklerinin tespitinde yüksek performanslı sıvı kromatografi HPLC yöntemi uygulanmıştır. Numune analizleri öncesinde farklı konsantrasyonlarda standart HMF çözeltileri hazırlanmış ve yönteme ait metot validasyon çalışmaları gerçekleştirilmiştir. Bal Tebliğinde TGKTebliğ No:20207 çiçek ve salgı ballarında HMF içeriği sınır değeri 40 mg.kg-1 şeklinde belirtilmektedir. Gerçekleştirdiğimiz analizlerden elde edilen sonuçlara göre numunelerdeki HMF değerleri 0.00-16.65 mg.kg-1şeklindedir. MH_MV3 ve MH_MV4 kodlu monofloral geven balı numunelerinde HMF değerinin en yüksek düzeyde olduğu belirlenmiştir. Sonuç olarak incelenen bal numunelerinin tümünün HMF açısından tebliğ ile uyumlu olduğu ve uygun bal kalite değerlerine sahip oldukları kanaatine varılmıştır.

Article Details

The Use of HPLC-DAD Method in Determining the Quality of Honeys: 5-Hydroxymethylfurfural (HMF) Analysis

Authors:

Merve Özbay | Fatma Nur Arslan | Gazi Görür

Publisher:

Karamanoglu Mehmetbey University

Main Topics:

Analitik Kimya (Diğer) | Gıda Mühendisliği

Journal:

Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 2, pp.41-45

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001325032

pISSN:

2687-5071

Floral honey

Honeydew honey

5-hydroxymethylfurfural

HPLCÇiçek balı

Salgı balı

5-hidroksimetilfurfural

HPLC

Issues

Article Details

Authors: Merve Özbay | Fatma Nur Arslan | Gazi Görür

Publisher: Karamanoglu Mehmetbey University

Main Topics: Analitik Kimya (Diğer) | Gıda Mühendisliği

Journal: Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 2, pp.41-45

Document Type: Article

Publication Date: 12/2024

Document Language: English

Document ID (Org No.): 2001325032

pISSN: 2687-5071

Issues

Authors:

Merve Özbay | Fatma Nur Arslan | Gazi Görür

Publisher:

Karamanoglu Mehmetbey University

Main Topics:

Analitik Kimya (Diğer) | Gıda Mühendisliği

Journal:

Karamanoğlu Mehmetbey Üniversitesi Mühendislik ve Doğa Bilimleri Dergisi Vol.6 Issue 2, pp.41-45

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001325032

pISSN:

2687-5071