Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

Hasan Hüseyin HADİMLİ*; Osman ERGANİŞ; Zafer SAYIN; Zeki ARAS

Article Details

Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

Authors:

Hasan Hüseyin HADİMLİ* | Osman ERGANİŞ | Zafer SAYIN | Zeki ARAS

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 4, pp.409-415

Document Type:

Article

Publication Date:

3/2012

Document Language:

Turkish

Document ID (Org No.):

2000766866

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (a, b, e, and i) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 (3.125%) was C. perfringens type B, 9 (28.125%) were C. perfringens type C, and 9 (28.125%) were C. perfringens type D. C. perfringens was isolated from 13 (8.66%) out of 150 intestinal samples. A total of 13 C. perfringens isolates from lambs were genotyped by PCR. Of the isolates, 10 (76.92%) were type A, 2 (%15.38) were type D, and 1 (7.69%) was type C. Type B and type E were not identified. The results showed that C. perfringens type A was the most common type by both ELISA and PCR in lambs with enterotoxemia.

 

Clostridium perfringens is a gram-positive, anaerobic bacterium that causes a wide range of diseases in humans and animals. The purposes of this study were to determine, using the enzyme-linked immunosorbent assay (ELISA), the clostridial toxins in intestinal samples of lambs with suspected enterotoxemia; to conduct molecular typing of C. perfringens isolates; and to investigate the presence of the genes of 4 major toxins (a, b, e, and i) in the isolates by polymerase chain reaction (PCR). According to the ELISA results, clostridial toxins were determined in 32 intestinal samples. Of the samples, 13 (40.62%) were C. perfringens type A, 1 (3.125%) was C. perfringens type B, 9 (28.125%) were C. perfringens type C, and 9 (28.125%) were C. perfringens type D. C. perfringens was isolated from 13 (8.66%) out of 150 intestinal samples. A total of 13 C. perfringens isolates from lambs were genotyped by PCR. Of the isolates, 10 (76.92%) were type A, 2 (%15.38) were type D, and 1 (7.69%) was type C. Type B and type E were not identified. The results showed that C. perfringens type A was the most common type by both ELISA and PCR in lambs with enterotoxemia.

Article Details

Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

Authors:

Hasan Hüseyin HADİMLİ* | Osman ERGANİŞ | Zafer SAYIN | Zeki ARAS

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 4, pp.409-415

Document Type:

Article

Publication Date:

3/2012

Document Language:

Turkish

Document ID (Org No.):

2000766866

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Key words: Clostridium perfringens

lamb

enterotoxemia

ELISA

PCR

Issues

Article Details

Toxinotyping of Clostridium perfringens isolates by ELISA and PCR from lambs suspected of enterotoxemia

Authors: Hasan Hüseyin HADİMLİ* | Osman ERGANİŞ | Zafer SAYIN | Zeki ARAS

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 4, pp.409-415

Document Type: Article

Publication Date: 3/2012

Document Language: Turkish

Document ID (Org No.): 2000766866

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Key words: Clostridium perfringens

lamb

enterotoxemia

ELISA

PCR

Issues

Authors:

Hasan Hüseyin HADİMLİ* | Osman ERGANİŞ | Zafer SAYIN | Zeki ARAS

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2012 Vol.36 Issue 4, pp.409-415

Document Type:

Article

Publication Date:

3/2012

Document Language:

Turkish

Document ID (Org No.):

2000766866

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128