Determination of Antioxidant and Antimicrobial Activities, Phenolic Composition and Volatile Compounds of Methanol Extracts of Erodium cicutarium (L.) L'Hér. Consumed As a Vegetable in Kilis

Filiz Uçan Türkmen; Gülcan Koyuncu; Fatma Esen Sarıgüllü Önalan; Ümit Haydar Erol

Article Details

Determination of Antioxidant and Antimicrobial Activities, Phenolic Composition and Volatile Compounds of Methanol Extracts of Erodium cicutarium (L.) L'Hér. Consumed As a Vegetable in Kilis

Authors:

Filiz Uçan Türkmen | Gülcan Koyuncu | Fatma Esen Sarıgüllü Önalan | Ümit Haydar Erol

Publisher:

Giresun University

Main Topics:

Mühendislik

Journal:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 4, pp.1460-1475

Document Type:

Article

Publication Date:

12/2023

Document Language:

Turkish

Document ID (Org No.):

2001305308

pISSN:

2564-7377

Keywords:

Abstract:

 The aim of this study is to investigate the antioxidant and antibacterial activities, phenolic profile and volatile compounds of methanol extract of Erodium cicutarium L. LHér, which is consumed as a vegetable in Kilis. The total phenolic substance content, total flavonoid substance amount, DPPH 1,1-diphenyl-2-picryl hydrazyl radical removal and ascorbic acid content values of the extract are respectively; 1.93 mg GAEg extract, 0.066 mg REg extract, 73.19 and 339.87 mgL. Antioxidant activity, metal chelating activity, reducing capacity and CUPRAC values by phosphomolybdenum method, respectively; 2.41 µgTE g, 86.13, 2.868 abs., 160.78 mg trolox equivalentg sample. Total antioxidant activity was calculated as  inhibition and trolox equivalent, these values are 51.40 and 9.56 µM trolox equivalent10g, respectively. According to the phenolic component results, the amount of naringin, the phenolic component that was detected in the highest amount, was 1143.8993 mgkg; the lowest detected component is t-ferulic acid and its amount is 5.08139 mgkg. Also, in the study, the two volatile compounds detected at the highest rate were phytol with 18.88 and 2-butoxy ethanol with 13.61; followed by p-xylene, undecane, neophytadiene and phthalic acid, butyl tetradecyl ester, respectively. In the determination of antibacterial activity, while no inhibitory effect was observed in extract against E. coli ATCC 25922 and Proteus spp. at a concentration of 100 mgmL; an inhibitory effect against Pseudomonas aeruginosa ATCC 9027 was observed, with a zone diameter of 6 mm. The results suggested that methanol extracts of E. cicutarium may be useful for food and medical applications.

 The aim of this study is to investigate the antioxidant and antibacterial activities, phenolic profile and volatile compounds of methanol extract of Erodium cicutarium L. LHér, which is consumed as a vegetable in Kilis. The total phenolic substance content, total flavonoid substance amount, DPPH 1,1-diphenyl-2-picryl hydrazyl radical removal and ascorbic acid content values of the extract are respectively; 1.93 mg GAEg extract, 0.066 mg REg extract, 73.19 and 339.87 mgL. Antioxidant activity, metal chelating activity, reducing capacity and CUPRAC values by phosphomolybdenum method, respectively; 2.41 µgTE g, 86.13, 2.868 abs., 160.78 mg trolox equivalentg sample. Total antioxidant activity was calculated as  inhibition and trolox equivalent, these values are 51.40 and 9.56 µM trolox equivalent10g, respectively. According to the phenolic component results, the amount of naringin, the phenolic component that was detected in the highest amount, was 1143.8993 mgkg; the lowest detected component is t-ferulic acid and its amount is 5.08139 mgkg. Also, in the study, the two volatile compounds detected at the highest rate were phytol with 18.88 and 2-butoxy ethanol with 13.61; followed by p-xylene, undecane, neophytadiene and phthalic acid, butyl tetradecyl ester, respectively. In the determination of antibacterial activity, while no inhibitory effect was observed in extract against E. coli ATCC 25922 and Proteus spp. at a concentration of 100 mgmL; an inhibitory effect against Pseudomonas aeruginosa ATCC 9027 was observed, with a zone diameter of 6 mm. The results suggested that methanol extracts of E. cicutarium may be useful for food and medical applications.

 The aim of this study is to investigate the antioxidant and antibacterial activities, phenolic profile and volatile compounds of methanol extract of Erodium cicutarium L. LHér, which is consumed as a vegetable in Kilis. The total phenolic substance content, total flavonoid substance amount, DPPH 1,1-diphenyl-2-picryl hydrazyl radical removal and ascorbic acid content values of the extract are respectively; 1.93 mg GAEg extract, 0.066 mg REg extract, 73.19 and 339.87 mgL. Antioxidant activity, metal chelating activity, reducing capacity and CUPRAC values by phosphomolybdenum method, respectively; 2.41 µgTE g, 86.13, 2.868 abs., 160.78 mg trolox equivalentg sample. Total antioxidant activity was calculated as  inhibition and trolox equivalent, these values are 51.40 and 9.56 µM trolox equivalent10g, respectively. According to the phenolic component results, the amount of naringin, the phenolic component that was detected in the highest amount, was 1143.8993 mgkg; the lowest detected component is t-ferulic acid and its amount is 5.08139 mgkg. Also, in the study, the two volatile compounds detected at the highest rate were phytol with 18.88 and 2-butoxy ethanol with 13.61; followed by p-xylene, undecane, neophytadiene and phthalic acid, butyl tetradecyl ester, respectively. In the determination of antibacterial activity, while no inhibitory effect was observed in extract against E. coli ATCC 25922 and Proteus spp. at a concentration of 100 mgmL; an inhibitory effect against Pseudomonas aeruginosa ATCC 9027 was observed, with a zone diameter of 6 mm. The results suggested that methanol extracts of E. cicutarium may be useful for food and medical applications.

 Bu çalışmanın amacı, Kiliste sebze olarak tüketilmekte olan Erodium cicutarium L. LHér ün metanol ekstraktının antioksidan ve antibakteriyal aktivitelerini, fenolik profilini ve aroma bileşimini araştırmaktır. Ekstraktın toplam fenolik madde miktarı, toplam flavonoid madde miktarı, DPPH 1,1-diphenyl-2-picryl hydrazyl radikal giderimi ve askorbik asit miktarı değerleri sırasıyla; 1.93 mg GAEg ekstrakt, 0.066 mg REg ekstrakt,  73.19 ve 339.87 mgLdir. Fosfomolibdenyum yöntemi ile antioksidan aktivitesi, metal şelatlama aktivitesi, indirgeme kapasitesi ve CUPRAC değerleri sırasıyla; 2.41 µgTE g, 86.13, 2.868 abs., 160.78 mg troloks eşdeğerig örnekdir. Toplam antioksidan aktivite ise  inhibisyon ve troloks eşdeğeri olarak hesaplanmış olup, bu değerler sırasıyla 51.40 ve 9.56 µM troloks eşdeğeri10gdır. Fenolik bileşen sonuçlarına göre, en yüksek miktarda tespit edilmiş olan fenolik bileşen naringinin miktarı 1143.8993 mgkg iken; en düşük tespit edilen bileşen ise t-ferulik asit olup miktarı 5.08139 mgkgdır. Çalışmada en yüksek oranda tespit edilen iki aroma bileşiği 18.88 ile fitol ve 13.61 ile 2-bütoksi etanol olurken; bunları sırası ile p-ksilen, undekan, neofitadien ve fitalik asit, bütil tetradesil ester takip etmiştir. Antibakteriyal aktivite tayininde, 100 mgmL konsantrasyonda E. coli ATCC 25922 ve Proteus spp.ye karşı özütlerde herhangi bir inhibitör etkiye rastlanmazken; Pseudomonas aeruginosa ATCC 9027ye karşı inhibitör etki gözlenmiş ve zon çapı 6 mm olarak ölçülmüştür. Sonuçlar, E. cicutariumun metanol ekstraktının gıda ve tıbbi uygulamalar için yararlı olabileceğini düşündürmüştür.

Article Details

Determination of Antioxidant and Antimicrobial Activities, Phenolic Composition and Volatile Compounds of Methanol Extracts of Erodium cicutarium (L.) L'Hér. Consumed As a Vegetable in Kilis

Authors:

Filiz Uçan Türkmen | Gülcan Koyuncu | Fatma Esen Sarıgüllü Önalan | Ümit Haydar Erol

Publisher:

Giresun University

Main Topics:

Mühendislik

Journal:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 4, pp.1460-1475

Document Type:

Article

Publication Date:

12/2023

Document Language:

Turkish

Document ID (Org No.):

2001305308

pISSN:

2564-7377

E.cicutarium

antioxidant

antibacterial

phenolic profile

volatile compoundsE.cicutarium

antioksidan

antibakteriyal

fenolik profili

aroma bileşikleri

Issues

Article Details

Authors: Filiz Uçan Türkmen | Gülcan Koyuncu | Fatma Esen Sarıgüllü Önalan | Ümit Haydar Erol

Publisher: Giresun University

Main Topics: Mühendislik

Journal: Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 4, pp.1460-1475

Document Type: Article

Publication Date: 12/2023

Document Language: Turkish

Document ID (Org No.): 2001305308

pISSN: 2564-7377

Issues

Authors:

Filiz Uçan Türkmen | Gülcan Koyuncu | Fatma Esen Sarıgüllü Önalan | Ümit Haydar Erol

Publisher:

Giresun University

Main Topics:

Mühendislik

Journal:

Karadeniz Fen Bilimleri Dergisi Vol.13 Issue 4, pp.1460-1475

Document Type:

Article

Publication Date:

12/2023

Document Language:

Turkish

Document ID (Org No.):

2001305308

pISSN:

2564-7377